| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,821 | C→G | 100% | C104S (TGC→TCC) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,821 | 0 | C | G | 100.0% | 31.8 / NA | 13 | C104S (TGC→TCC) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (7/6); total (7/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACG > NC_000913/2661693‑2661957 | cgtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGCCGGTCGCTCAAATAACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGccc > 2:235860/1‑143 (MQ=255) tcgtGAGTATGCTGACGACCAGCCAAATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTg < 2:249148/143‑1 (MQ=255) tATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCACGGATTTATCGCCGCCCTGCCAgctg > 1:305512/1‑141 (MQ=255) ttCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGGCGGTAAGGGATGTATCGCCACCCTGGCAGCCGACTATACCCTGACAACGGGTGGCATCTa > 2:247129/1‑143 (MQ=255) tGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAACGCGTGGGTCAGGGATTTATCGCCGCCCTCGCAGCCGCCTTTACCCTGACAACGGATGGCATCtcaaga > 2:20997/1‑138 (MQ=255) gTAATGTTGTTGAGAAGACCGGGGAGACGGTCGCTGAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTc < 2:126444/143‑1 (MQ=255) tgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCCACAGATTCGTCACCGGGCCTAATTACTGCGCCAACgcag > 2:135633/1‑140 (MQ=255) gggaAATCCGGTGCGACGGTCGCTCCAAGCTCGCCACAGCGCGTGGGTCGGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGa < 2:333804/141‑1 (MQ=255) agaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCTGCTACAGATTCCTCAAGGGCGCTTATTACTTCGCCTACGGCgtt > 1:164612/1‑141 (MQ=255) cAAAGCACGCCACAGCGCGTGGGTCAGGGATTTAGCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACa < 1:73737/143‑1 (MQ=255) ggcacgGCGCGTGGGTCAGGGAGTTATCGCCGCCCTGGCAGCCGCCTTTGCCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAacca < 2:52508/141‑1 (MQ=255) cgcgTGGGTCAGTGCTTTATCGCCGCCCTGGCAGCCGCCTTTGCTCTACCAATGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTg < 2:237022/143‑1 (MQ=255) gTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACg > 2:239130/1‑143 (MQ=255) | CGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACG > NC_000913/2661693‑2661957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |