| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 143,534 | G→A | 12.9% | L252L (CTG→CTA) | yadG → | putative ABC transporter ATP‑binding protein YadG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 143,534 | 0 | G | A | 12.9% | 59.9 / 2.2 | 30 | L252L (CTG→CTA) | yadG | putative ABC transporter ATP‑binding protein YadG |
| Reads supporting (aligned to +/- strand): ref base G (15/11); new base A (2/2); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01 | |||||||||||
TCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTT > NC_000913/143395‑143670 | tCGGCATTATTCAACACGGTGAGCTGGTGGCAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGTAACCTTTATTCTCGATCTCGAACCGAAAAGCACGTTACAGAAGCACGATGGCTATCAGTATAGAATGGtg > 1:30536/1‑142 (MQ=255) ttCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTGTTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACCAGTCGATActgat < 2:341395/143‑5 (MQ=255) ttCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGACTATCAGTATCGACTGGTCGATACcgcg > 1:84729/1‑143 (MQ=255) ggTGAGCTGGTGGAAAATACCTAGATGAAGTCGCTGCTTGCGAAGCTGAAATAGGTATGCCGAACACACGATGTAGCGCCGAAAACCCCGTTACCGCAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGACACGCTGGa > 2:75406/1‑143 (MQ=255) cgggtggAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGAGCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGa < 1:151757/141‑1 (MQ=255) tggtggAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGGCGATACCCCGAAGCAGGAAGATGaa > 2:222468/1‑143 (MQ=255) cTCGCTGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCACGCTGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGCAGtgctgccagacc > 2:10030/1‑137 (MQ=255) aTGAAGGCGCTGCTGGCGATGCTGTATTCTTAATCTTTTATTCTCGATCTCGCACCGAAAAGCCCGGTACCGAAGGGCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAggg < 1:42939/143‑1 (MQ=255) ggaGGCGCGGCTGGCGGTGCGGAAATCGGACACCTTTATTTGCGAGTTCGCCCCGAAAAGCCCGTTACCGTGGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGa < 2:130840/141‑1 (MQ=255) gatggCGCTGCTGCCCAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGa < 2:84729/140‑1 (MQ=255) aaGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTAGAAGTGCTGCGTGAGCAGGGGAt > 2:300753/1‑143 (MQ=255) agcggctgctGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAACGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGCCTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATc < 2:119461/139‑1 (MQ=255) gttgctgGCAAATCCAAAATCGAAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACa < 1:202354/141‑1 (MQ=255) gctgctGGCGAAGCTGAAATCGGAATCCTTTATTCTTGATCTCGCACAGAAAAGCCCGTTACCGAAGCTCGATGGCTAGCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACa < 1:202256/143‑1 (MQ=255) tgctgGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGCAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCAACGCAGGAGGGTGAAGTGATGCGTGAGCAGGCGATCAACaac > 2:20914/1‑141 (MQ=255) gctgGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCg < 1:257832/143‑1 (MQ=255) aGCTGAAATCGGGAACGTTTAGTCCCGATCCCGCACCGAAAAGCCCGTTAGCGAAGCTCGAGGGGTATCAGTATCGACTGGTGGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACg < 2:331650/143‑1 (MQ=255) tGAAATCGGAAACCTTTATTCTCGATCTCGCAAGGAAAAGCCCGTTACCGAAACTCGATGGCTATCAGTATCGACTGGTCGATACTGCGACGCTAGAAGTTGAACAGCTACGAGAGGAGGGAAGCAACAGCGTAGTAACGAAg > 2:224858/1‑143 (MQ=255) gAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGCAGTTGAAGTGCTGCGTGAGCAGGGGATCAACCGCGTATTTACGCCGTTAAGTGa > 1:209482/1‑143 (MQ=255) tccTTTATTTTCGTTCTCGTACTGTATTGCCTGTTAGCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCa < 1:310605/142‑1 (MQ=255) aactctcttCACGAGCTCGCACCGAAAATCCCGTTACAGAAGCTCGATGTCTATCAGTATCGACTAAACGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGAGATCAACAGCGTATTTACGCAGTTAAGTGAGCa < 1:75406/136‑1 (MQ=255) tATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGAGCAACAGCGTATTTACGCAGGTAAGTGAGCAGGGCa > 1:157449/1‑143 (MQ=255) tctcGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCCATCAGTATCGCCTGGTCGATACCGCCACGCTGGAAATTGAAGTGCTGCGTGCGCAGGGGATCAACAGCCTCTTGACGCAGTTAAGGGAGCTGGGCAtgc > 2:225914/1‑141 (MQ=255) gCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCACGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGAAGGGCATTCAGGTATTAAg > 2:224152/1‑143 (MQ=255) cccGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGTGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACa > 1:181648/1‑143 (MQ=255) ccGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGCCGATACCGCGACGCTGGAAGAAGAAGTGCTGCGAGAACAGAGGATCAACAGCATATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACaa > 1:218823/1‑143 (MQ=255) tATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCACTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCt > 1:182104/1‑143 (MQ=255) gcggTATCGACCAGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCtgt > 1:289891/4‑143 (MQ=255) gcggTATCGACCAGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGAGAAATAAGATATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCtgt > 1:119949/4‑143 (MQ=255) tCGACTGGTGGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTgtttgttt < 2:96234/143‑1 (MQ=255) | TCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTT > NC_000913/143395‑143670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |