| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,634 | A→C | 100% | T108P (ACC→CCC) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,634 | 0 | A | C | 99.4% | 74.8 / ‑7.0 | 27 | T108P (ACC→CCC) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (15/11); minor base G (1/0); total (16/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTA > NC_000913/3041498‑3041768 | ttGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGCGTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAACAATATGCGGTATTCTCTACCGTGCCCGTcg > 1:50728/1‑143 (MQ=255) cGACGCCAAAGGTAAAATGTGTAGCAATTTACGTCTGTTCCGCGACGGCGTTTGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCgcgc < 1:171770/143‑1 (MQ=255) aCGCCAAATGTAAAATGTGGAGCGATTTACGGCTGTTTCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCa < 1:182868/143‑1 (MQ=255) aCGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATAGCCTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGAAGATGAGGAAAGTGAGAAAATATGCGGTATTATCTAAAGTGGCCATCGCGCCa > 2:142160/1‑143 (MQ=255) ccGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTGTCTAAAGTGCCCATCGCGCCAGACGACGAGCGGTGGCTGCTTGGGGTTGCCGCGTTTc > 1:16821/1‑143 (MQ=255) ggCTTTGCATGGATGGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGATTGTTTTTGCCGGTTTTCAGGCGcgcgccgc < 2:220089/143‑1 (MQ=255) tttGCCTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCTCCAGACGACGAGCGTGTGCTGCGTGGTGTGGCCGGTCTGCCGGCGcgcgcagcgcg > 2:308368/1‑142 (MQ=255) ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGTCTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg < 1:254345/143‑1 (MQ=255) agtgggcggcggAGCGTGCGTGAACCGCAGCTGACTGACCTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGCGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct < 1:124740/137‑1 (MQ=255) ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAGAtctc > 1:43546/1‑143 (MQ=255) ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTATTCAGGCGCGCGCCGCGCTGGCAAAtctc > 1:218158/1‑143 (MQ=255) tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGCTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct > 1:57606/1‑143 (MQ=255) cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGCCGCGCGCCGCGCGGGCAAATCTCtttcg > 2:64823/1‑141 (MQ=255) cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGCCAAATCTCTTTACCGAACTCCATTc > 2:154984/1‑143 (MQ=255) tgaaAAAATATGCGGTATTCTCTAAATTTCCTTTTTCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCAGTTTTAAAGATCTCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa < 1:50643/143‑1 (MQ=255) tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt > 2:175121/1‑101 (MQ=255) tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt < 1:175121/101‑1 (MQ=255) tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa > 1:298050/1‑143 (MQ=255) aaaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCAGGGGGTCAAACGCGCAAAGATGCGGAAGGTCTTTTTGGCGAACTGCCTTCGAAAGAAAAACAGGACGTCAacga > 2:75370/1‑143 (MQ=255) aaaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGCTTTTGAGGCGCGCGCCGCCCTGGCAAATATCTTTAGCGAACTGCCTTCGAACGAAAAACAAGTAGTCAaaga > 1:43896/1‑143 (MQ=255) agaagtatGCGGTATGCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa < 1:276600/138‑1 (MQ=255) caacttcgcgcTATTCTCTAGATGGCCCGTCGCGCCGGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCACGCGCGCGCCGCGCTCGCAAATCTCTTTAGCGAACTTCCTTCGAAATAAAAACAGGTAGTCAaagaag < 2:271623/136‑1 (MQ=255) aaTATGCGGTATTCTCTAAAGTGCCCAGCGCGCCAGACGACGACCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTGAGCGAACTACCTTCGAAAAAAACACAAGTAGTCAAAGAGGgc > 2:195272/1‑143 (MQ=255) tATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGGTTTTGTCCCCAACAAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACt > 1:264327/1‑143 (MQ=255) tctgcaaaGTGCCCCGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg < 2:277703/138‑1 (MQ=255) ctAATGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa < 2:213531/143‑1 (MQ=255) ctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGACAGAAACACAGGTAGTCAAAGCAGGCGCGACCACTTTGCTa > 1:216005/1‑143 (MQ=255) | TTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTA > NC_000913/3041498‑3041768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |