| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,121,334 | G→A | 20.0% | intergenic (‑55/+300) | glcA ← / ← glcB | glycolate/lactate:H(+) symporter GlcA/malate synthase G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,121,334 | 0 | G | A | 20.0% | 31.5 / 3.8 | 20 | intergenic (‑55/+300) | glcA/glcB | glycolate/lactate:H(+) symporter GlcA/malate synthase G |
| Reads supporting (aligned to +/- strand): ref base G (9/7); new base A (2/2); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
GGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGTTAA > NC_000913/3121217‑3121474 | ggCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGATCTATAAAata > 1:308869/1‑143 (MQ=255) agCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTAACTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGCCGTta > 2:122027/1‑143 (MQ=255) gCCCCAGTCCTTCCTTCGGCATTTACTTTTGTGTCCAGTTAACCATATTGTTCAGTCTCTTATTATCTTTAGATGCTTTATATACTTAAGGTTGTAATAAGCAAAAGAGGACTTAACTGTAAAATATAGGCGTTATACTTTAc < 2:200294/143‑1 (MQ=255) tcctccCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACa > 2:212386/1‑143 (MQ=255) ccttCGGCATATACATTTGGGTCCAGGTAACCATATTGTTGAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTAcg < 1:73145/140‑1 (MQ=255) ataCATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAACAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGc > 2:275511/1‑143 (MQ=255) ataCATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAACAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGc > 2:273485/1‑143 (MQ=255) taCATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCa > 2:77187/1‑143 (MQ=255) gTAACCATATTGTTCAGTCTCTTATTAGCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGc < 1:234284/143‑1 (MQ=255) tctTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTa > 2:221463/1‑143 (MQ=255) atCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGttt < 2:169909/143‑1 (MQ=255) cTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAACAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCAAACGCAATTGCTCCCTCTGGCATAACAAGTATATCGGGTCAggccctct > 1:239765/1‑137 (MQ=255) taCTTAAGGTTGTAATAAACAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacgcaga < 1:275511/143‑1 (MQ=255) taCTTAAGGTTGTAATAAACAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacgcaga < 1:273485/143‑1 (MQ=255) cTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAATATATAGGCGTTATACTTTACAGCAACAGTACTCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACacgcagacg < 1:276088/143‑1 (MQ=255) ttAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAGTATAGGCGTTATACTTTACAGCCACCGTACGCCGCTAACGCAATAGATACATATAGCATAAAAAATATATCGGGTAAGGGTTTCTGTTGCGCACaagcagagga > 1:212702/1‑140 (MQ=255) ggTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGACGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCCAACGCagag > 2:344537/1‑143 (MQ=255) ataaGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACATCATCAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGtt > 1:346670/1‑143 (MQ=255) aaGCGAAAGAGGACTGAGATGTAAGATATAGGCGGGATGCGTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGTCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGTTaa < 1:243927/143‑1 (MQ=255) aaGCAAAAGCGGACTGAACGGGAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCGAACGCAATTGCTACCTCTGGCATAACAAGTATAGCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGTTaa < 2:285596/143‑1 (MQ=255) | GGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGGTAACCATATTGTTCAGTCTCTTATTATCTTTATATGCTTGATATACTTAAGGTTGTAATAAGCAAAAGAGGACTGAACTGTAAAATATAGGCGTTATACTTTACAGCAACAGTACGCCGCTAACGCAATTGCTACCTCTGGCATAACAAGTATATCGGGTAAGGGTTTCTGTTCCGCACACGCAGACGCAGAGTATCGTTAA > NC_000913/3121217‑3121474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |