| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,991,432 | C→T | 23.6% | P94S (CCA→TCA) | cyaA → | adenylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,991,432 | 0 | C | T | 23.6% | 19.8 / 3.6 | 17 | P94S (CCA→TCA) | cyaA | adenylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (3/10); new base T (2/2); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01 | |||||||||||
CTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGA > NC_000913/3991294‑3991574 | ctgaTGCCGTGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAAt < 2:249458/140‑1 (MQ=255) aTGCCGGGGTACCTTGATGGGAACGTACCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCGGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTAc < 2:9995/143‑1 (MQ=255) ccTTGATGTTAACTTTTGCAAAGGTATTTGTTTTTTCATGCTTGATTAAACTCAACGTCATTTCCTGATTGAGCTTGAACTGTATCGTGGAATGTAAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATaca < 2:191331/143‑1 (MQ=255) cTTCATGGTAACGTTCCCAAAGGCATTTGCCTGTACACGCCTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTCTCGTGGAACGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATacac < 1:47963/143‑1 (MQ=255) tGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGTAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATgg > 2:177531/1‑143 (MQ=255) ggTAACGTTCCCAAATGCATTTGCCTTTACACGCCTGATGAAATTCAACGCCACTACCTTAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCagtaa < 1:334571/143‑5 (MQ=255) tCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCt < 1:207021/143‑1 (MQ=255) aaaGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTGAACGAGATTGAACGGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTc > 1:363328/1‑143 (MQ=255) gaAACTCAACGCCACTACCTAAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCTTCCGTAGGGCAAAGTTGTTCCTCTGACCt < 2:119506/143‑1 (MQ=255) aaCGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTTCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGTGAAGTTGTCCCGCTTACATgaaaaaa > 1:148066/1‑137 (MQ=255) aaCGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTTCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATc > 1:64880/1‑143 (MQ=255) aCGTCACTGCCTGAACGAGCTTGGACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCt < 2:240709/143‑1 (MQ=255) aCGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGACGAAAGGAGAGCTTCCAATTACTGGTGTATACACCATGCACAGCACCTCGTCCGTAAGGCAACGCTGTTCCTCTGACCTGGAAATCt > 2:46595/1‑143 (MQ=255) acaccccggACTGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTAGACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATa < 1:162699/136‑1 (MQ=255) cgAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCGCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTaaa < 2:81874/143‑1 (MQ=255) tggggAGCTTTGAATTACTGGTGTATACACCATTGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTa < 2:148066/139‑1 (MQ=255) aGGTGAGCTTTCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTa < 2:64880/143‑1 (MQ=255) ccAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGa < 1:246259/143‑1 (MQ=255) | CTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGA > NC_000913/3991294‑3991574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |