| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,155,549 | C→A | 34.0% | T183T (ACC→ACA) | argC → | N‑acetylglutamylphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,155,549 | 0 | C | A | 34.0% | ‑0.3 / 4.8 | 12 | T183T (ACC→ACA) | argC | N‑acetylglutamylphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base C (2/6); new base A (2/2); total (4/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.64e-01 | |||||||||||
GGAGTGGTGCGGTAATAAATTAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATCGCCATCAACCAGAGATCGCCACACACCTCGGTGCTGACG > NC_000913/4155408‑4155676 | ggAGTGGTGCGGTAATAAATTAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGCTCTTCTTGACGTCAATCAGTCGACGGTGATCAACGccacca > 1:212366/1‑143 (MQ=255) aGTGGTGCGGTAATAAATTAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTAGCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAgc < 1:139335/143‑1 (MQ=255) aaggagCGAATGTGATGGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACTTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGt < 2:78975/143‑1 (MQ=255) cGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCCACGCCACAAGCGGCGTGAGCGGTGCTGGGCGTCAAGCg > 1:82021/1‑143 (MQ=255) cGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACAAGCGGCGTGAGCGGTGCAGGGCGTAAAGCg > 1:364344/1‑143 (MQ=255) acgacgGCACAGCTGGCGCTGAAACCATTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGTAAAACGACCAATTCAAACAGCTGTTGTGAAGTTAGCCt > 2:93140/1‑143 (MQ=255) gTTGATTTATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATGGGGGGCGCCACCCACCAGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATc < 1:361906/143‑1 (MQ=255) ttgattgaTGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATCg > 1:68727/1‑143 (MQ=255) tcaCCCCAACCAGCAGCCGGTGATCAACCCCACCAGCGGCGGGAGCGTTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATCGCCATCAACCAGAGATCGCca < 2:133524/141‑1 (MQ=255) agctctATCAGTGGCCGGTGATCAACGCCACAAGCGGCGTGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATCGCCATCAACCAGAGATCGCcaca < 2:82021/141‑1 (MQ=255) aCCTCAATCAGTGGCCGGTGATCAACGCCACAAGCGGCGTGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATCGCCATCAACCAGAGATCGCcaca < 2:364344/143‑1 (MQ=255) ccGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATCGCCATCAACCAGAGATCGCCACACACCTCGGTGCTGa < 2:34364/143‑1 (MQ=255) ggTCATCAGCGCCACCAGCGGCGTGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGCTAGCCTGCAACCGTATGGCGTCTTTACTCATCGCCATCAACCAGAGATCGCCACACACCTCGGTGCTGACg < 2:196452/143‑1 (MQ=255) | GGAGTGGTGCGGTAATAAATTAAAAGAAGCGAATTTGATTGCGGTGCCGGGCTGTTATCCGACGGCGGCACAGCTGGCGCTGAAACCGTTGATTGATGCCGATCTTCTTGACCTCAATCAGTGGCCGGTGATCAACGCCACCAGCGGCGTGAGCGGTGCAGGGCGTAAAGCGGCCATTTCAAACAGCTTTTGTGAAGTTAGCCTGCAACCGTATGGCGTCTTTACTCATCGCCATCAACCAGAGATCGCCACACACCTCGGTGCTGACG > NC_000913/4155408‑4155676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |