| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 256,454 | C→G | 16.2% | intergenic (+19/‑73) | gpt → / → frsA | xanthine‑guanine phsophoribosyltransferase/fermentation‑respiration switch protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 256,454 | 0 | C | G | 16.2% | 65.5 / 10.5 | 31 | intergenic (+19/‑73) | gpt/frsA | xanthine‑guanine phsophoribosyltransferase/fermentation‑respiration switch protein |
| Reads supporting (aligned to +/- strand): ref base C (16/10); new base G (3/2); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-02 | |||||||||||
CGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTA > NC_000913/256315‑256595 | cGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGc > 1:208893/1‑143 (MQ=255) gCAAAACCGGCAGGTCGTCCGCTGGTAGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGcc < 1:83388/143‑1 (MQ=255) ccGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGACGCTAATCTTTTCAACGCCTGGCACTGCCGTGCgc > 2:173743/1‑142 (MQ=255) tcgtcgtcCGCTGGATGATGACTATGGTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttgaaa < 1:33936/141‑4 (MQ=255) ccgtcCGCTGTTTGATGCCTACGTCGTTGATATCCCGCAAGATGTTTGTCTCTAACACCCGTGGGTCATACTCGCCTTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttt < 2:159400/142‑1 (MQ=255) tcCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAACCTTTTCAACGCCTGGCACTCCCGGGCGTTGCTCTTTTTa > 2:173822/1‑143 (MQ=255) atCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCag < 1:78948/143‑1 (MQ=255) cccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagta > 1:177198/1‑143 (MQ=255) aTACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTATCAACGCCTGGCACTGCAGGGCGATGTTGTTTTTAACTTCAGGCGGGTAACAATAGTTTCAAGCAAGTAGTCt > 2:174591/1‑143 (MQ=255) ttGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCAGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAAGCGGGTTACAATAGTTTCCAGTCAACATTCTTGAGGCTGc > 2:305967/1‑143 (MQ=255) ttGAACAGCCGTGGGATATGGACGTCGTATTAGTCCCGCCAATCTCCGGTCGGTAATCTTAAAAACGCCTGGCACTTCCACACGTTGTTCATTTTAACTTCAGACGAGTTCAAACACTTTCCAGCAAGTATTATAGAGACTGc > 2:274063/1‑143 (MQ=255) aaCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATATACGGTCGCTACTCTTTTCACCGACTAGCACAGACGGGCGGTGATCTTTTTAACTACGGGAGGGTTACAATAGATTCCAGTAAGTCGTCTGGAGGCTGcgtg > 2:269056/1‑140 (MQ=255) cAGTCGTGGGATATGTGCGGCGTAGTCGTCCGGCCAATCTCCGGTCGCTAAGCTGTTGAACGGCGGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCa < 1:58016/143‑1 (MQ=255) gggATATGGGCGTCGTATTCGTCAGACGAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACGTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGAcaca < 1:138076/143‑1 (MQ=255) tcggtgtcgtATTCGTCTTGCTAATCTCCGGTCGCTAATCATTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCaaa < 1:4124/138‑1 (MQ=255) cGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCCTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAAcc < 2:177198/143‑1 (MQ=255) cccGCCAATCTCCGGACGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACTGGCAAACCTGAGCGACAcccag > 2:307524/1‑141 (MQ=255) ccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGt > 2:106004/1‑143 (MQ=255) ccGCCAAGCGCCGGTCGCTAATCTGTTCAACGCGTGGCACTGCCGGGCGTTGTTCTTTGTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGt < 1:96226/143‑1 (MQ=255) gcGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCg < 2:190923/142‑1 (MQ=255) ccGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGGATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGGAACCCTGTTCAAACCCCg > 2:55834/1‑143 (MQ=255) ggTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGACAAACCTGAGCGAAACCCTGTTCAACCCCCACt > 2:334306/1‑143 (MQ=255) ttttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCGAACCTGAGCGAACCCCTGTTCAAACCCCGCTTTAAACATCCt > 1:252068/1‑143 (MQ=255) cAACGCCTGGCCCCGCCGGGCGTTGTTCCTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGaaa < 1:270914/143‑1 (MQ=255) aaCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTAAAATAGTTTCCAGTAAGTATACTGAAGGCTACAACCATGACACAGGCAAACCAGAGCGAAACCCTGTTCAAACCACGCGTTAAACAACCTGAAAc > 2:304210/1‑143 (MQ=255) aaCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGt < 2:137205/115‑1 (MQ=255) aaCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGt > 1:137205/1‑115 (MQ=255) aaCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAAc > 2:113806/1‑143 (MQ=255) aCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGCGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGCAAcc > 1:1607/1‑143 (MQ=255) cGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCt > 2:24385/1‑143 (MQ=255) ggCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACg > 2:182532/1‑143 (MQ=255) tcTGCCGGGCGTTGTGCTTTTTGACTTCGGGCGCGTTACAATAGTTGCCAGTAAGTATTCTGGAGGCTGCACCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTa < 2:282969/142‑1 (MQ=255) | CGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTA > NC_000913/256315‑256595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |