| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 319,152 | C→T | 27.4% | A284T (GCA→ACA) | rclA ← | putative pyridine nucleotide‑disulfide oxidoreductase RclA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 319,152 | 0 | C | T | 27.4% | 19.1 / 5.8 | 18 | A284T (GCA→ACA) | rclA | putative pyridine nucleotide‑disulfide oxidoreductase RclA |
| Reads supporting (aligned to +/- strand): ref base C (6/7); new base T (2/3); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01 | |||||||||||
TTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTG > NC_000913/319022‑319290 | tttttCCGATCATCAGTACTACGTTTACCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCGAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAATATCTCCCATCGCCCAAATCTTGTCTGCGGTGGTATGTaa > 2:347010/1‑143 (MQ=255) ttttCCGACCATCAGTACTACGATAGCGTTCACCCGGCAATTAATCACATACAATGCGGTAATCATCCCGGGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAAt < 1:80173/143‑1 (MQ=255) ctatcatcAGTACCCCCTTCTCCATCACCTACTAATTCACCACTCACATTGCGCTAATCATCCAGTGATATTCACGTAAATTGCAGTACGCCTCCAACATCTCCCATCTCCCAAATATTGTCTGCGGTGGTATGTAATCGCtt < 2:8803/141‑1 (MQ=255) aGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTATAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGa < 1:177064/143‑1 (MQ=255) aCGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACAGCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCgg < 1:147153/143‑1 (MQ=255) aaTGCGGTAATCATCCAGCGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAACTATTGTCTGCGGTGGTATGTAATCACTTGTGAACGACAATAGCCACGCGATCAGATACTGCGATAACGACagatt > 1:65683/1‑139 (MQ=255) tGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAAAATTGTCTGCGGTGGTAGGTAATCGCATGGCAACGACAATTACCACGCGCTCGTGTGCTACGATACCGGCATTTTCTGGATAGAACGGAGCgg > 2:109679/1‑143 (MQ=255) tGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCACAAATATTGTCTGCGGTGGTATGGAGTCGCTTGTCAAAGACAATTGCCCCGTGCTCGTGTACTCCGATACAGGCAATAACTGGATATAAAGaccagg > 2:80980/1‑138 (MQ=255) gtcggtacATTGCGGCCCGCCGGTAACATCTCCCATCGCCCGAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTGTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCg < 2:68252/139‑1 (MQ=255) ttcAGCCCGCCGGTAACATCCCCCACCGCCCAAATATTGTCTGCGGTGGTATGTACTCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGAcc < 1:68892/141‑1 (MQ=255) gagctcCCATCGCCCAAAAATTCTCTGTGGTGGTAGGAAAGCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTAGTGCGATACCGGAATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGt < 2:254669/140‑1 (MQ=255) cATCTCCCATCGCCCAAATATTGTCTGTGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGc > 1:173595/1‑115 (MQ=255) cATCTCCCATCGCCCAAATATTGTCTGTGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGc < 2:171788/115‑1 (MQ=255) cATCTCCCATCGCCCAAATATTGTCTGTGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGc < 2:173595/115‑1 (MQ=255) cATCTCCCATCGCCCAAATATTGTCTGTGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGc > 1:171788/1‑115 (MQ=255) ctcCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCCTTTTCTGGATGTACCGTAGGGCCACCCGCTTGGTGACCGGGAGCTAGTAACAGTGCa > 2:255105/1‑143 (MQ=255) cccAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCAACCACCGcc > 2:199935/1‑143 (MQ=255) gccgCGGTGGTATGTAATCGCTTGCCCACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTg < 1:128316/140‑1 (MQ=255) | TTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTG > NC_000913/319022‑319290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |