| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 754,446 | A→G | 100% | L8P (CTC→CCC) | gltA ← | citrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 754,446 | 0 | A | G | 100.0% | 35.6 / ‑6.7 | 17 | L8P (CTC→CCC) | gltA | citrate synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (6/10); minor base T (0/1); total (6/11) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAA > NC_000913/754309‑754576 | cGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTTAACAGCAGTATCACCGTTGAGGGTGGGtttt > 1:100393/1‑143 (MQ=255) gATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAAACGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCGATCAGCACATACAGTCAAACAGCTGTATCCCCGACGAGAGAGGGATTTGCtaa > 2:300453/1‑141 (MQ=255) aTGCTGTTGAAGTGAAGCCTGGGTCATAGGTGAACACACCTTGCGAACCGATAGTACCGAGATCAATAACAGCGTGACCCAGCGTGCCCTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt < 2:314322/143‑1 (MQ=255) aTGCGGTTGAAGTGAAGCCTGGGTCAAATGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCGTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt < 1:355242/143‑1 (MQ=255) agttaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCa < 2:288285/143‑1 (MQ=255) tGAACACACCTTTTGAACAGAGAGTACGGATATCAATAACATCTTGACCTAGCGTGCCTTTTAGCACATACAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGcc < 1:41585/143‑1 (MQ=255) acacCCTTTGCACCGAGAGCACGTATATCCATATCATCTTGCCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt < 2:269040/143‑1 (MQ=255) agTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAAGGTGGGTTATGCATTTGAATCAGCCATTTAAGGTCACCTTAGCGCCTTATTGCGTTAGACTGCCGGaa > 1:337131/1‑143 (MQ=255) gATATCAATAACATCTGGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaaa < 2:242509/143‑1 (MQ=255) tctaaTAACATCTTGCACCAGCGTGCCATGCAGCACACCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt < 1:122990/140‑1 (MQ=255) cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGcc < 1:7627/104‑1 (MQ=255) cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGcc > 2:7627/1‑104 (MQ=255) gTTCATCTGCCGTATCCCCTTTGACGTTGTGTTTTGTGTTTGTATCAGCCATTTACGGTCTCCTTAGCGCCTTTTTGCGTAAGTCTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTgg < 2:10526/143‑1 (MQ=255) aCAGCTGTATCCCCGTTGAGGGTGGGTTTTGGTTTTGTATCAGCCATTTAAGTTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgc < 2:236094/143‑1 (MQ=255) aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTGCCCGTTTTTTCTTTGGCTctgct > 1:107146/1‑139 (MQ=255) atgcccGTTGAGGGTGGGTTTTGCGTTTGTATCAGCCACTTAAGGCCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTTCCCGTTTTTTATTTGGCTCGCCGCTCtgt < 2:158931/140‑1 (MQ=255) ccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaaa > 2:88838/1‑143 (MQ=255) | CGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAA > NC_000913/754309‑754576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |