| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | 100% | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 98.6% | 31.3 / ‑6.9 | 15 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (7/7); minor base A (1/0); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCA > NC_000913/124102‑124374 | tggtggTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAgg > 1:104256/1‑143 (MQ=255) ggtgggCACGAGCCGAGGAAAATCTACGCGGCATACAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTGGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGt < 2:152116/143‑1 (MQ=255) cGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAAc > 1:181301/1‑143 (MQ=255) aagaaAATCTACGCTGCATTCAAGATAGCGCACGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt < 2:84723/143‑1 (MQ=255) ttCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCatat > 1:177537/1‑143 (MQ=255) ttCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGCCatac > 1:73799/1‑142 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCATTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGAAAAAACATCGCGCACAAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg > 2:44386/1‑143 (MQ=255) gaGGCAAAGGGACAGTAATCCTTGCTCATACCATTAAAGGTGACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg < 2:54072/142‑1 (MQ=255) acacTAGTCCTTGTTCATATCATTAAAGGTTATGGCTTAAACTCCTCGACTAAAGATAAAAACATCGCGCACTAGGTAAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGc < 1:114167/143‑1 (MQ=255) tACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATCTCCGCGACCGTTTCAATGTGCCGGTGTGTGATGCGGATATCGAAAAACTGc > 1:105828/1‑143 (MQ=255) ttACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACTGTTTCGATGTTCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCCCCt > 1:17661/1‑143 (MQ=255) gCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGAGTCGGAGCCTCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAGCTGGCGGACATCAGGTGCCGGGAAGGt > 1:76013/1‑143 (MQ=255) gtGAAGGTAAAAACATCGCGCACTAGGTTAGGAAAATGAACATGTACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAagag < 2:201483/142‑1 (MQ=255) aactgcgcgCACTAGGGTAAGAAAATGAACATGGCCGGGGTGCGTCATATCCGCGACCGTTTCTGTGTGCCCGTTCCCCATGCCGCTATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCt < 2:177537/138‑1 (MQ=255) cgcgcgCACTAGGTTAAGAAAATGAACATGGGCGGTGTGCGACATAGCCAGTACGATTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCa < 1:37446/141‑1 (MQ=255) | TGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCA > NC_000913/124102‑124374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |