| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,473,614 | C→T | 100% | W156* (TGG→TGA) | rpsG ← | 30S ribosomal subunit protein S7 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,473,614 | 0 | C | T | 90.9% | 21.6 / ‑3.8 | 12 | W156* (TGG→TGA) | rpsG | 30S ribosomal subunit protein S7 |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (6/4); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCA > NC_000913/3473478‑3473701 | tGTTACGGTAGCGTGCGATGGGTGTTGTCCGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGGTCAATTTACGTAGCCCAAAGCGGGCTGCTTCCTGGAGGCGCCCGCCTGGTGCCTAAACCTCCGACGGGATAATCACCgc > 1:186641/1‑142 (MQ=255) aaTTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATATAAGTAGCCCAAAGCGGGATGCTTACTGGAAGCACCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGAGCGCGAACGCCTTGATGGATTCGGCCAtca > 2:63255/2‑141 (MQ=255) gttCCTCGTTTATCTTTTCGGCGTTCAATTTAAGGAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGaa < 1:176196/142‑1 (MQ=255) ttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAAGCAACGGTAGTGTGCGAACGACTTGATGTCTGCGACCATACGGTGAAc > 1:136636/1‑143 (MQ=255) ttCCTCGTTTATCTTTTAGGCATTCAATTTATGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAACACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTCGTTGGCTTCGGCCATACGGTGAAc > 2:123574/1‑143 (MQ=255) aTCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGt > 1:133900/1‑143 (MQ=255) aGGCGTTTAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTaa < 2:116595/143‑1 (MQ=255) tttaaTTTAAGTAGCCTAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCTGAAGTGTTAATCAACGGTAGTGTGCGAACGCCTGGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCa < 1:164719/140‑1 (MQ=255) gtCAATGTAAGTAGCCCAAAGCGGGCTGCGTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTGGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCa < 1:38137/142‑1 (MQ=255) aTTTAAGTAGTCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAggtc > 1:164729/1‑140 (MQ=255) aGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGCACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTg > 1:101969/1‑143 (MQ=255) aaGCGGGCTGCTTACTGGAAGCGCCTTCCGGGTGACTAAAACTCCGAAGGGATAATCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgca < 2:87552/143‑1 (MQ=255) | TGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCA > NC_000913/3473478‑3473701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |