| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 461,007 | T→G | 100% | I707S (ATC→AGC) | lon → | Lon protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 461,007 | 0 | T | G | 85.1% | 29.7 / ‑2.6 | 16 | I707S (ATC→AGC) | lon | Lon protease |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base G (6/7); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGAT‑CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAA > NC_000913/460872‑461141 | gTGACACCCACGTCCACGTCCCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGAG‑CACTCTg < 1:129586/143‑1 (MQ=255) acgtccacgtACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCCCCGCGCTGGTTTCTTGCCTGACCGGTAACCCGCTTCGTGCCGAAGTGGCAATGACCGGTGAGAG‑CACTCTGCGTGGTCAg < 1:128476/143‑1 (MQ=255) acgtccacgtACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGAG‑CACTCTGCGTGGTCAg < 2:186807/143‑1 (MQ=255) gtccacgtACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCCATGACCGGTGCGAG‑CTCTCTGCGTGGTCAGGt > 1:184187/1‑143 (MQ=255) gacgtACCGGAGGGTGGGACGGCGAGTGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTGCTTGCCTGACCGGTAACGCGGTTCGTGCCGATGTGGCAATGACAGGTGAGAG‑CACTCTGCGTGGTGAGGTACt < 2:43629/142‑1 (MQ=255) cccGGAAGGGGCGACGCCGAGCGATGGTCCGAGTGCCGGTATTGCTATGTGCCCCGCGCTGGTTTCTTGCCTGACCGGTACCCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGAG‑CACTCTGCGTGGTCAGGTACTGCCGa < 2:149730/142‑1 (MQ=255) ggTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACGGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGAG‑CCCTCTGCGTGGTCAGGTTCTGCCGATCggtgg > 1:139582/1‑143 (MQ=255) gTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGCTGTGGCAATGACCGGTGAGAG‑CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGGTTGAAAGAAAAACTGCTGGCAGCGCATcgc > 2:74624/1‑143 (MQ=255) gCACCGCGCCGGCTTCGTGCCTCACCGCGAACCCGGTGCGTGCCGATGTGGCAATGACCGGTGAGAG‑CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTaaa < 1:60677/143‑1 (MQ=255) cgCCGGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGAG‑CACTCTGCGTGGTCAGGTACTGCCTATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGtt < 1:194711/142‑1 (MQ=255) aCCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGAT‑CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAACCAGTGCTAATTCCGTTCGaaaa > 1:128907/1‑143 (MQ=255) aCCCGTTGCGTGCCGATGTCGCAATGACCGGTGAGAT‑CACTCTGCGTGGTCAGCTACTGCCGATCGGTGGTTTGAACGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACTGTGCTAATTCCGTTCGAAAATAAAcgc < 2:16302/143‑1 (MQ=255) cGGTTCGTGCCGATGTGGCAATGACCGGTGAGAG‑CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGTGCATCGCGGCGGGCGTAAAACAGGGCTAATTCCGCTCGACAATAAACGCGac > 2:190919/1‑142 (MQ=255) gaggcGAGGTGTCAATGACTCGTG‑GATGCACACTGCGTGGTCAGCTACTGCCGATCGGTGGTTAGAAAGAAAAACGCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGaa < 2:8695/139‑1 (MQ=255) cGGTGAGAG‑CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGGTCCGGAAGAGATTCCTGACAACgaca > 2:30442/1‑140 (MQ=255) cGGTGAGAG‑CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGAACTGGAAGAGATTCCTGCAAACGTaa > 2:19513/1‑143 (MQ=255) | GTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGAT‑CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAA > NC_000913/460872‑461141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |