Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,032	IS1 (–) +8 bp	intergenic (‑110/‑488)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	37 (0.890)	33/284	0.2	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 1293039	0 (0.000)					intergenic (‑117/‑488)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
*	?	NC_000913	1293032 =	0 (0.000)	44 (1.060)	39/284	0.1	100%	intergenic (‑110/‑495)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

CGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/258048‑257908 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTG  <  NC_000913/1293039‑1292899                                                                                                                                                                                                                                                                                            CGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCG                                                                                                                                             >  1:445794/1‑143            gtATCTGAGTCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAA                                                                                                                                  <  1:235357/141‑1             AATCTGAGGCAGCACTGGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAATTTGTTTTAGTTTTATGACAAAGTCATCGGGCATTATGTGAACATAAAACACTATCAAGAAGTTGGAGTCATTACCCGGCACAAAATAAA                                                                                                                                 <  1:281936/143‑1               TCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTCTCTGAACATAAAACACGATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGA                                                                                                                               >  2:519734/1‑143                       AGCACCTGGCACGGCTGGGACGGAAGTCGCTGGCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTT                                                                                                                       <  2:727785/143‑1                          ACCTGGCACGGCTGTGACGGCAGGCGCTGTCGTCCTCAAAACCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATCAAACACTATCAATAAGGTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAA                                                                                                                    <  1:89771/143‑1                          ACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAA                                                                                                                    <  2:383893/143‑1                               GCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAAAACAACTTTGAATTCCT                                                                                                               >  1:665861/1‑143                                 ACGGCTGGGACGGGAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGTATTATCTTAACATAAAACACTATCAATGAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTA                                                                                                             <  2:275928/143‑1                                     cttGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATCTTGCATACCTTACATc                                                                                                         >  2:353320/4‑142                                      TGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGACATTACCCGGCACAAAATAAAGAACAATTTTGAATTCGTTACATTC                                                                                                        >  2:325809/1‑143                                         GACGCAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTG                                                                                                     <  2:455481/143‑1                                                   GCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCAC                                                                                           >  1:447577/1‑143                                                                AAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAATACACTTTTATTTTTTTGGTGTCTTTTCCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAG                                                                              <  1:449517/143‑1                                                                         GAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACCTTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTA                                                                     >  2:499799/1‑143                                                                           GCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCTATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATT                                                                   <  1:390434/143‑1                                                                              GCATGACAAAGTCATCGGGCATTATCTGAACAAAAAACACTATACATAAGTTGGAGTCATTACCCGGCACCAAATAAAGAACAATTTTGACTTCCTTACATTCCTGGCTATTACACAACTAAATTTAAGCCTCTATTATTACa                                                                >  1:648562/1‑142                                                                                  GACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAATGCTCTATTATTACCTCta                                                            >  1:126246/1‑141                                                                                             CGGGCTTTATCTGACCGTAAAACGCTATCAATAAGTTGGAGTCATTACCCGGCACACAATAAAGAACAATATTGATTTCCTCACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCGCAACAAACCACCCC                                                 <  2:10399/143‑1                                                                                                GCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGAACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCACAACAAACCACCCCAAT                                              >  2:47959/1‑143                                                                                                 CATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATA                                             >  1:316020/1‑143                                                                                                        TGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTT                                      <  2:451348/143‑1                                                                                                             attagtCACCATCAATAAGTTGGAGTCAGTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGAT                                 <  1:664344/137‑1                                                                                                             ATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAAGCTCTATTATTACCTCAACAACCCACACCAATATAAGTTTGAGAT                                 >  1:121134/1‑143                                                                                                              TAAAACACTATTAACAAGTTGGCGTTATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTTCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATT                                <  2:730354/143‑1                                                                                                               gAAACACTATCATTAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTA                               <  2:22244/142‑1                                                                                                                AAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTAAAATTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTAC                              >  1:211623/1‑143                                                                                                                    ACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAATTTTGAGATTACTACA                          >  1:425372/1‑143                                                                                                                      TATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGCACAATTTTGAATTACTTACTTTGCTGGGTTTTGCACAGGTGAATTTAAGGCTCTATTATTACTTCAACAAACCACCCCAATATAAGTATGAGATTACTACAAT                        >  1:385837/1‑143                                                                                                                              AGTTGGAGTCATTACCCGGCACACTTTAAAGATCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCAGCCCAATAGAAGTTTGAGATTACTACAATGAGCAAAG                >  2:482800/1‑143                                                                                                                                     GTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAA         >  1:500239/1‑143                                                                                                                                      ttATTACCCGGCACAAAATAAGGAACAATTTTGAATTTCTTATATTCCTGGCTATTGCACAACTGAATTTAAAGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAAATTACTACAATGAGCGAAGCACTTAAc        >  2:34865/3‑142                                                                                                                                      TCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCCACAAACCACCCCCATATAAGTTTGAGAATACTAAAATAAGAGAATTACTTAAA        >  1:381805/1‑143                                                                                                                                      TCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATAGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAACATAAGTTTGACATTACTACCATCCCCGAAGCACTCAAA        >  2:143371/1‑143                                                                                                                                      TCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCGATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAA        <  1:192096/143‑1                                                                                                                                           ACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCT   <  2:445794/143‑1                                                                                                                                            CCCGTCACAAAATAAACATCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTG  <  2:431980/143‑1                                                                                                                                                                                                                                                                                            CGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/258048‑257908 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTG  <  NC_000913/1293039‑1292899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.