| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 97.2% | 85.1 / NA | 36 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (16/19); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTAT > NC_000913/124102‑124369 | tgggggTCACGATCCGAAGAAAATCTACGCTGAAATAACGCGATCTCTGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAgg < 2:344754/143‑1 (MQ=255) gATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGATAAGAAAAt > 1:693486/1‑143 (MQ=255) tCCGAAGCAAATCTACGCTGCATTCAAGAAAGCGAAGGAAACGAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCCGAAGGTAAAAAAATAGCGCACTAGGGCGAGAAAATGa > 2:677739/1‑143 (MQ=255) ccGAAGAGAATCTACTCGGCATTCAAGATAGCGCAGGATACCAAAGGCAGAGCGTGAGTACCCGTTGCTCGTACCATTAAAGGTTACGGCATGGGTGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGaa < 2:7662/143‑1 (MQ=255) ccGAAGAAAATCTACGCTGCATTTAAGATATCGTAGTTTTTCTAGGGCAAAGGGACAGTAAGCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGaa < 1:540052/143‑1 (MQ=255) ccGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGCCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGCGCGGCGCGGCCGCAGGTAAAAACATCGCGCACTAGGCTAAGAAAATGaa > 2:146829/1‑143 (MQ=255) gaagaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAGGGCAAAGCGACAGTAATCCTTGCCCATAGCAATAAAGGTTACGGCATGGGCGACGAGGCTGAAGGCAACAACATCCCGAACCAGGCTAAGAAAAGAAACa > 2:39717/1‑143 (MQ=255) aagaaAATCTACTCTGCATTCAAGAAAGCGCATTAAACTAATGGCTGTTGGACAGTTTTTCTTTCTCATATCATTAAATGTTACGGCATGGGCGTCGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt < 1:106265/143‑1 (MQ=255) aacagaaTCCACGCCGCATTCAAGAAAGCGTAAGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGTCATGGGCTACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt < 2:754711/138‑1 (MQ=255) cTGCGTTGAAGAAGGAGCAGGAAACCAGAGGCAAAGCGACAGTAATCCTTGCTCATACCCTTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAGACGGCGCGCACTAAGTTAAGAAAATGAACATGGACGGTGTGCGt < 2:385943/143‑1 (MQ=255) aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgc > 1:374813/1‑143 (MQ=255) aGAAAGAGCAGAAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGCTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgc < 1:392385/143‑1 (MQ=255) gAAAGCGCAGGAAACCAAAGGCACAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAATATCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgcg < 2:524386/143‑1 (MQ=255) taaGCGCAGGAATCCAACGGCATAGCGACAGTAACCCTTGCTCATAAGATTATAGTCTAAGGCATGGGCGACGCGGTTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGa < 2:563834/142‑1 (MQ=255) cAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATAGCATAAAAGGTTACGGCATGGGCGCCGCGGCTGACGGTAAAAACAACGCGCACTGGGTTAAGAAAGTGAACATGGACGGTGTGCGGAATAGCTGGGACCGgtt > 2:628751/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGCAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATCGACGGTGTGCGTCATATCCGCGACAGATTCaa > 1:435357/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCCTCTCCGCGGCCGTTTcca > 1:316869/1‑141 (MQ=255) aaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATTGACGACACAACAAAAAGTAAAAACATCACGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg > 1:590231/1‑143 (MQ=255) gCAAAGCGACAGTAATCCTTGCTCATACCCTTAAAGGTTACGGCATGGGCGACGCGGTTGAAGGTACACACATAGCGCACTAGATTAAGAAAATAAACATGGACGGAGTGCGAAATATCCGAGACCGTTTCAATGTGCCGgtg > 1:95159/1‑143 (MQ=255) gCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAAGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGgtg > 1:449298/1‑143 (MQ=255) aCAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGGGTCTGATGc > 2:769468/1‑143 (MQ=255) tAATCCTTGCTACTACGATGAAATGTTACGGCATGGTCGACGCGGCTGAATGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGat < 2:8500/143‑1 (MQ=255) gaTCCTTGCTTATACCTTTAATGTTTATGGCATGGGCGACGCGGCGGAAGGTAAAAACATAGCGCAGTAAGTTAAGAAAAAGAACAAGGACGGTGTGCGTCATATCCGCGACCGTTGCAATGTGCCGGTGTCTGATGCAGata < 1:22240/142‑1 (MQ=255) ccTTGCGCATACCACTAAAGGTGACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCg < 1:424846/143‑1 (MQ=255) ttAATGGTTACGGCATAGGCGACGCGGCTGAAGATAAAAACATCGCGCACTACGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGCCCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTAc < 2:480368/143‑1 (MQ=255) gCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGATAAGAAAATGAACATGGACGGCGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCg > 2:438868/1‑143 (MQ=255) gCATAGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCg < 1:666162/143‑1 (MQ=255) ggCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATCTCGAAAAACTGCCGTACATCACCTTCCCGGAAgg > 1:605298/1‑143 (MQ=255) cGACGCGGCTGAAGGTAAAAACATCTCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGtt < 1:754066/143‑1 (MQ=255) cgcgGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATACCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAACCACTGCCGTACACCACCCTCCCCGCTGGTTCTg > 1:170434/1‑143 (MQ=255) gcggAAGTTAAAAACTTCGCGCACTAGTTTAATAAAATGAACATGGACTGTGGGCGTCATATCCGCGTCCGTCTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAaga < 2:656690/140‑1 (MQ=255) gCTGAAGGAAAAAACAACGCGCACTAGGTTAAGAAAATGGACATGGACGGTGTGCGTCAGATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAaga < 1:15171/143‑1 (MQ=255) aaacaaaaaCATCGCGCACTAGGGTAAGAAAAAGAACATGGACGGTGTGCGTCAGATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCTCCTTCCCGGAAGGTTCTGAAGAGCATa < 1:773805/139‑1 (MQ=255) aGGTAAAAACTTCGCGCACTAGGTTAAGAAAATGAACGTGGACGGTGTGAGGCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATa < 1:733312/143‑1 (MQ=255) aaaaaCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACAGACCACCATGACGTTCCCGGAAGGTTCTGAAGAGCATAccaa > 1:325859/1‑141 (MQ=255) aaaaCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCAGTACATCACATTCCAGGAAGGTTCTGAAGAGCATACatat > 1:34047/1‑143 (MQ=255) | TGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTAT > NC_000913/124102‑124369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |