Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,368,101	T→C	A21A (GCT→GCC)	arnA →	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,368,101	0	T	C	93.8%	36.7 / NA	16	A21A (GCT→GCC)	arnA	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (10/5);  minor base A (1/0);  total (11/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

GATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATC  >  NC_000913/2367961‑2368231                                                                                                                                             |                                                                                                                                   gctCTACACCGAGGTCCGTGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGc                                                                                                                                  <  2:457940/141‑1 (MQ=255)    cTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTAGTCTTTGCCTACCACGATATGGGATGCCTCGGCATTGAAGCGCTGCTGgccgccag                                                                                                                               >  2:428198/1‑141 (MQ=255)                            ccGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGGTACGAAATTAGCGCCATTTTTAc                                                                                                       >  2:397590/1‑143 (MQ=255)                                            cAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATccc                                                                                       >  1:380596/1‑143 (MQ=255)                                               gTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGt                                                                                    >  2:788445/1‑143 (MQ=255)                                               gTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTAGTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGt                                                                                    <  2:380596/143‑1 (MQ=255)                                                         catccaGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTAGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTTAGAATCCCGGTGAAGAAAcca                                                                          >  2:1583/1‑142 (MQ=255)                                                           tccaGCAAGGAAAATGAATAATGAAACCCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCTTGTTGGCCGCTGGTTACGAAATTAGCGACATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCttt                                                                        <  1:379581/143‑1 (MQ=255)                                                                   ggAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGTTGCCTCTGTATTTAAGCCCTGCTGTCAAACGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTc                                                                >  1:245724/1‑143 (MQ=255)                                                                   ggAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTGCTGGTTc                                                                >  2:678917/1‑143 (MQ=255)                                                                     aaaaTGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAACTTAGCGCCATTTTTACCCATACTAATAATCACGGTGAAAAAGCCTTTTATGGTTCgg                                                              >  1:164203/1‑143 (MQ=255)                                                                      aaaTGAATAATGATAACCGTCGTTTTTGCCTACCACGGTATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCTTGCTGCTAATCCCGGTGAAAAAGCCTTTTATGGTTCGGt                                                             <  2:435817/143‑1 (MQ=255)                                                                                                    tACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTcc                               >  1:582676/1‑143 (MQ=255)                                                                                                        aCGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAACGCCTTTTCTGGTTCGGTGGCTCGTCTGGCCGCGGAAAGAGGCATTCAggat                           >  1:116787/1‑141 (MQ=255)                                                                                                                ggATGCTTCGGTATTGATGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGTTAATCTCGTTGATAAATCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGcc                   <  1:711223/143‑1 (MQ=255)                                                                                                                      cTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAAGCATTCCGAGTTATGCGTCGGaaaa             >  1:684444/1‑143 (MQ=255)                                                                                                                                 aGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGCCATTCCGGTCTATGCGCCGGACAACCTTAatcatc  >  2:646475/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                   GATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATC  >  NC_000913/2367961‑2368231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑