Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,821	C→G	C104S (TGC→TCC)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,821	0	C	G	100.0%	112.3 / NA	36	C104S (TGC→TCC)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (20/16);  total (20/16)

GGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGT  >  NC_000913/2661681‑2661958                                                                                                                                             |                                                                                                                                          gggggggTGGGGCGTCGTGAGAATGCTGACGACCAGAAACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGAt                                                                                                                                         <  1:704101/137‑1 (MQ=255) gggTGTGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGAt                                                                                                                                         <  1:774633/143‑1 (MQ=255)   gTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGGGAGAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGAttt                                                                                                                                       <  2:794013/143‑1 (MQ=255)      cGTGGCGCGTCGTGAGTATGCTGACGAACAGACACATCCAGCACTTCCTGGTTATTAACCAATTCGCCTAAGGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCCACAAACACGCCACAGCGCGTGGGTCAGGGATTTATc                                                                                                                                    >  2:132941/1‑143 (MQ=255)           cgcgTCGTGAGTACGCTGACGACCAGACACAGCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAGCGTACGGTGGTTGAGGGAGCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcgccgc                                                                                                                               <  2:92579/143‑1 (MQ=255)               ccgggggTATGCCGACGACCGGACACATCCAGTACTTACTGGTCATTAACCAGTTCGTCTAAAGTAATGTTGTTGAGAACACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTg                                                                                                                           <  2:735416/137‑1 (MQ=255)                          cTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCtt                                                                                                                <  1:771302/143‑1 (MQ=255)                            gacgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCttaa                                                                                                              >  1:479388/1‑141 (MQ=255)                            gacgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCAATGGCAGCCGCCTTTa                                                                                                              >  1:610357/1‑143 (MQ=255)                               gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCCCAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAccc                                                                                                           >  2:768134/1‑143 (MQ=255)                                  cAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCCAGCAGCCGCCTTTACCCTGa                                                                                                        >  2:503171/1‑143 (MQ=255)                                          tCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTACTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTCTCCCTGACCACGGGt                                                                                                >  1:331990/1‑143 (MQ=255)                                           ccAGCACGTCCCGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg                                                                                               <  1:347365/143‑1 (MQ=255)                                            cAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAGTCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTGCCCTGACGATGGGTgg                                                                                              >  1:620503/1‑143 (MQ=255)                                             aGCACTTCCTTGTTATTAACCAGTTCGCCTAAAGTAATGTGGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGc                                                                                             <  1:656841/143‑1 (MQ=255)                                               cgcgtCCTGGTTATTACCAAGGTCGCCTAAAGGAGTGTTGTTGAGGAAACCGGGGAGACGGTCGCTCAAATCGCGCCACAGCGCGTGGGTCAGGGATTTATGGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCAt                                                                                           <  1:792062/139‑1 (MQ=255)                                                 cTTCATGGTTATTAACTAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCt                                                                                         <  1:750225/143‑1 (MQ=255)                                                   tCCTGGTTATTTTCCAGTTCGCCTAAAGTAATGTTGTTGAGAAGACCGGTGAGACGGTCGCTCGGGTCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTAc                                                                                       <  1:402820/143‑1 (MQ=255)                                                     cTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAg                                                                                     >  1:471131/1‑143 (MQ=255)                                                                          tAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGCCTATCGGTGGCGTCTACAGCTTCGTCAACGCCGCTaactc                                                                >  2:559864/1‑140 (MQ=255)                                                                          tAAAGTAATGTTGTTGAGAAAACCGGTGAAACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCGGGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGCTTCGTGAACGACGCtgctca                                                                >  2:193469/1‑138 (MQ=255)                                                                              gTAATGTTGTTTAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATCTAGCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTc                                                            <  1:236320/143‑1 (MQ=255)                                                                              gTAATGTTGTTGAGAAAAACGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTGCCCCTGACAACGGGTGGCATCGACAGATTCGTGAGCGGCCCTAATTACtgc                                                            >  2:533580/1‑141 (MQ=255)                                                                                            aaaaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAAAGGCGAtgc                                              >  1:762058/1‑143 (MQ=255)                                                                                                           gTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc                               <  2:713714/143‑1 (MQ=255)                                                                                                               cTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGAAAAAAAACTACAAATTCGTCAACGGCGCTAATAACTTCGCCAAGGGCGATGCTGCTGGCATCTTTGCCTaa                           >  1:313644/1‑143 (MQ=255)                                                                                                                      cACGCCACATCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGTCTTTACCTTGACAACGGGTGGTATCTACAGATTCGTCAACGGCGATAATGACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATaa                    <  1:54738/143‑1 (MQ=255)                                                                                                                      cACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGACATCTTTGCCTAACAGATaa                    >  1:634716/1‑143 (MQ=255)                                                                                                                      cACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGACGATGCTGCTGGCATAATAGCCTAACAGATaa                    >  1:481386/1‑143 (MQ=255)                                                                                                                       aCGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGCTTCGTCACCGGCGCTAATTACTTCGCCCCCGGCGATGCTGCTGGCATCTTTGCTCAACAGATAac                   >  1:186122/1‑143 (MQ=255)                                                                                                                            acaGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGCTGCTTCTGGCCTCCTTGCCTAACAAATAaccaaca              >  1:6944/1‑140 (MQ=255)                                                                                                                            acaGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCg              >  1:561466/1‑143 (MQ=255)                                                                                                                              aGCGCGTGCGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCACCTACAGATTCGTCAACGGCGATAATTACTTCGCCAACGGCGATGCTGCTGGAAACTGTGCCTAACAGATAaccccagaa            >  2:75411/1‑137 (MQ=255)                                                                                                                                        tCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTCCAGATTCGTCAACGGCGCTAATTACTTCGCCACCGGCGATCCTGCTCGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGt  >  1:276660/1‑143 (MQ=255)                                                                                                                                        tCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAATGGTGATGTTGTTGGCATCTTTGCCTAACAGAGAACCACCGCCTGGTCCACGt  <  1:47749/143‑1 (MQ=255)                                                                                                                                        gcAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGt  <  2:120152/142‑1 (MQ=255)                                                                                                                                             |                                                                                                                                          GGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGT  >  NC_000913/2661681‑2661958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑