Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,634	A→C	T108P (ACC→CCC)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,634	0	A	C	100.0%	137.0 / NA	48	T108P (ACC→CCC)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/24);  total (24/24)

CATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041496‑3041767                                                                                                                                           |                                                                                                                                      cATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGCACTGCAAAAATTTGCGGTATTCTCTAAAGTGCCCAt                                                                                                                                   >  1:349043/1‑143 (MQ=255)    tGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATcgc                                                                                                                                <  1:684221/143‑1 (MQ=255)      cGATGCCAATGGTAAAATTTGGAGCATTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCgcgc                                                                                                                              <  1:18718/143‑1 (MQ=255)           ctaaaGGTAATATGGGGACCAAGTAACGTCTGTTCCGCGACGGCGATGGCGATGCATGGATTGAACGGCGCAGCGGGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgac                                                                                                                         <  1:213340/141‑1 (MQ=255)             aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacga                                                                                                                       >  2:68117/1‑143 (MQ=255)             aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacga                                                                                                                       >  2:67695/1‑143 (MQ=255)                ggTAAAATGTGGAGCAATTTATGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGAcgacga                                                                                                                    <  2:219101/143‑1 (MQ=255)                attAAAATGTGAAGCAATATACGGCCGAGCCGCGACGGCGATCGCGCACAACTGCTACAACTGCGCAGCGGGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGAcgacga                                                                                                                    <  1:177109/141‑1 (MQ=255)                 gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGTGCCCATCGCGCCAGAccacgac                                                                                                                   >  1:136334/1‑142 (MQ=255)                    aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGGGAACCGCAGCTGACTGAACTGAAAAAAGAGGCGGTATCCACCACAGGGCACATATGGCCAGAcgacgatggc                                                                                                                >  2:557027/1‑139 (MQ=255)                       tgtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGtgtg                                                                                                             <  1:412286/143‑1 (MQ=255)                        gtgAAGCCATTTACCTCTGTTCCGCGCCGGCGATAGGCATCCATGGATTTAACCGCGCAGCGTGCGTGAACCGCAGCTGTCTGAACTCAAAAATTATGCGGTATTCTCTAAAGTGCCCATCGCGTCAGACGACGAGCGTgcgc                                                                                                            <  2:47571/143‑1 (MQ=255)                              cattttACGTCTGTTCCGCGACGGCGATGGCTGTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                      <  1:5283/140‑1 (MQ=255)                              cAATTTACGTCTGTTCCGCGACGGCGCTGGCTTTGCATGGATTGAAAGGCGCACCGTGCGTGAACCGCAGCTGACTGACCTGACAAAATATGCGGTCTTCCCTAAAGTGCCCATCGCGCCAGCCGACGAGCGTGTGCTACTTg                                                                                                      >  2:387219/1‑143 (MQ=255)                              cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGATGCTTg                                                                                                      >  1:509228/1‑143 (MQ=255)                                    aCGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGAATACTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTggcattg                                                                                                >  2:666926/1‑138 (MQ=255)                                     cGTCTGTTCCGCGAGGGCGATGGCGTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                                                               <  1:550070/143‑1 (MQ=255)                                       tCTGTTCCGCGACGGCGATGGCGTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                             <  1:453307/143‑1 (MQ=255)                                         tGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGGATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt                                                                                           <  1:604836/143‑1 (MQ=255)                                          gTTCCGCGACGGCGATGGCTTTGCATGGCTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAACAAATATGCGGTATTCTCTAAAGTGCCCATCGCGACAGATGACGGGCGTGTGCTTTTTGTTGTTGCCGGtt                                                                                          <  2:569653/143‑1 (MQ=255)                                          gTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt                                                                                          <  1:96584/143‑1 (MQ=255)                                           ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAACTATGCGGTATTCTCTCAAGTGCCCACCGCGCCCGACGTCGAGCGTGCGCTGCGTGGAGTTGACggaga                                                                                         >  1:261980/1‑140 (MQ=255)                                                 gACGGCGATGGCATGGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgc                                                                                   <  1:759430/143‑1 (MQ=255)                                                      cGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATCTGCGGGATTCGCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcg                                                                              <  2:276135/143‑1 (MQ=255)                                                            cTTTGCATGGATTGCCCGGCGCGGCCGGCGTGAACCGCAGCTGACTGAACTGATAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcgc                                                                        <  2:373059/143‑1 (MQ=255)                                                             tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGAAGGTTTTCAGGCGAGCGCCGCGCt                                                                       >  1:311877/1‑143 (MQ=255)                                                             tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTAAAAGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATAGCGCCAGACGACGAGCAAGTGCTGCTTGGTGTTGACGGTTTACAGCCGagcgccgcgcg                                                                       >  2:53889/1‑142 (MQ=255)                                                                  aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAGTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGGCGGCGGGCGTGTGCTGCTGGGTGTTGGCGGTTTTCAGGGGCGCGCCGCGCGGGcgg                                                                  >  1:159741/1‑141 (MQ=255)                                                                  aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGAGTTGCCGTGTGTCAGGCGCGCGCCGCGCTGGCaa                                                                  >  2:489071/1‑143 (MQ=255)                                                                    cggttGAACGGCGCAGCGTGCGGGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                                                                <  2:465157/140‑1 (MQ=255)                                                                      aTTGAATGGCTCATCGTGCGTGAACCGCAGCTGACTGAACTGCAAGAACAAGCGGGATTCTCTCAATGTCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                              <  1:106305/143‑1 (MQ=255)                                                                        ggAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                            <  2:422002/142‑1 (MQ=255)                                                                           acgggtcAGCTTGCGTCAATCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGTGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCTCCGCGCTGGCAAATCTCTTTa                                                         <  2:660525/137‑1 (MQ=255)                                                                             ggCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTCGc                                                       >  2:102542/1‑143 (MQ=255)                                                                               cgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCCCTGGCAACTCTCtttctcga                                                     >  1:751454/1‑138 (MQ=255)                                                                                      tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGAGCCCGCCTGGCAAATCTCTTTAACGAAATGCCt                                              >  1:658359/1‑143 (MQ=255)                                                                                      tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGAAGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCACGCCGCGCTGGCAAATCTCTGTAGCGAACTgaca                                              >  2:127508/1‑140 (MQ=255)                                                                                         gtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGTGCCGCGATGGCAAATCTATTTAGCGAACTgagttag                                           >  1:123139/1‑137 (MQ=255)                                                                                          tgATCCGCACCTGGCTGAACTGAGACAATGTGCGGCAGTTTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTTCTTGGTGTTGCCTGTTTTCAGGCGCGCGTCGCGCTGGCAAATCTCTTTTGCGAACTGCCTTCga                                          <  2:703809/143‑1 (MQ=255)                                                                                          tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCGTCga                                          >  1:554639/1‑143 (MQ=255)                                                                                                        ctgcacggaaAAAAGATGCGGTATCCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                            <  1:110967/143‑1 (MQ=255)                                                                                                              tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTCCGAAAGCAATACAGGTAGTCaca                      >  1:459034/1‑141 (MQ=255)                                                                                                              tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGATGTTGCAGTTTTTCAGGCGCGCGCCGCGCTGGCAAAGCTCTTCAGCGGACTGCCTCCGAACGAACACCAGGTAGtacac                      >  2:127907/1‑139 (MQ=255)                                                                                                                      tatGCGGTATTCTCTAAAGGGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCTGGTTTTCAGGCGCGCGCCCCTCGGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgcgc              <  1:800475/143‑1 (MQ=255)                                                                                                                        tGCGGTATTCTCTAAAGTGCCCATCGCGCCAGAAGATGTGAGTGAGCGCCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAGCTGCCCTCGAACGAAACACAGGCAGTCAAAGAAGACGCGa            >  2:351048/1‑143 (MQ=255)                                                                                                                        ggCGGTATTCGGAAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGAGCTGGAAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa            <  1:168334/142‑1 (MQ=255)                                                                                                                                  tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTAGCt  >  1:377001/1‑143 (MQ=255)                                                                                                                                  tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGATTTAAGTCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCATCGAAAGAAAAACAGGCAGTCAACGAAGGCGCGACCACTTTgat  >  2:523481/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                      CATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCT  >  NC_000913/3041496‑3041767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑