Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	760,485	G→A	G594S (GGC→AGC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	760,485	0	G	A	96.5%	64.5 / NA	29	G594S (GGC→AGC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/14);  minor base C (1/0);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACT  >  NC_000913/760354‑760619                                                                                                                                    |                                                                                                                                       agaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGCTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaa                                                                                                                             >  1:396235/1‑143 (MQ=255)  gaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATAGCGATCGCCAGGCGATGACTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaa                                                                                                                            >  1:10937/1‑143 (MQ=255)     gAAGCGACTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAcc                                                                                                                         <  1:384677/143‑1 (MQ=255)       ggcgcTTGCAGTGGCGGGCGACACGCAGCGGTGCGGGGCCGGAAGCAGTTGAAATGCAGTGTCGTGTTGCCAAGCATTATGGCTATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc                                                                                                                       <  2:195344/142‑1 (MQ=255)        gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGCGAGAAACTGTTCGACTGCAGCGGTGCGGCAAAccccc                                                                                                                      >  2:319175/1‑140 (MQ=255)                   aGCTGGCGAAACGCATCAGCAAGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGAGTTATGGCGATCGCCAGGCGATGGCTGCGGGCGAGAAACTGAACGACTGGAGCGGTGAGCGAAACCTCGCATACGCCagc                                                                                                           >  2:320685/1‑141 (MQ=255)                       ggCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACACTgg                                                                                                       >  1:721342/1‑143 (MQ=255)                                   cAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCa                                                                                           >  1:789208/1‑143 (MQ=255)                                     gCACGGTGCCGGAATCAGTTGAAATGCAGTCTCGCTTTGCCAAGTTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCAtt                                                                                         <  1:256437/143‑1 (MQ=255)                                         agTGCCGGAAGCAGTTGAAGTGCAGTCTGTCGTTGCCAGGTTTTATGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAGGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCgg                                                                                     <  1:796158/142‑1 (MQ=255)                                          gTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGt                                                                                    >  2:388499/1‑143 (MQ=255)                                               ggAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGACCGCGCCTACGCCACGCTGGTTGAGGAAGGCATTCCGGTCCGcc                                                                               >  2:563562/1‑143 (MQ=255)                                                     aGTTCAAGTGCAGTCTCGCGTAGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg                                                                         <  2:133287/143‑1 (MQ=255)                                                          aaaTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCAACAACCGATGGCAGCAGATGAGAAACTGTTCGACTGAAGCGATGCGGAAAAACAGGCTAACGCCACACTGGATAATGAAGGCATGCAGGTTCGGCTGGCggtttaa                                                                    >  2:54391/1‑138 (MQ=255)                                                             tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTACGGAAAACATCGCTTACGCAACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGAc                                                                 >  1:648135/1‑143 (MQ=255)                                                                      gcgTTGCCACGATTTATGGCGATCGCGAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgc                                                        <  2:583010/143‑1 (MQ=255)                                                                          tGCCAAGCTTCCTGGTAATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTa                                                    <  1:490845/143‑1 (MQ=255)                                                                          tGCCAAGATTTATTGCTATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTa                                                    <  1:215704/143‑1 (MQ=255)                                                                              aaGATTTATGGCGATCGCCAAGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGATTCGCCAGTCGGGTGAAGACTCCGGTCGCGGTACctt                                                >  2:501853/1‑143 (MQ=255)                                                                               aGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCAGTCGCGGTACcttc                                               >  1:645888/1‑143 (MQ=255)                                                                                gATTTATGGCTATCGCGAGGCGATGGCTGGCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACcttct                                              <  2:105649/143‑1 (MQ=255)                                                                                           atcccgaGGCGATGGATGCCGGTGAGACCGTGTTCGTCTGGAGCGCTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAc                                   <  2:460790/137‑1 (MQ=255)                                                                                           aTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAc                                   <  2:434550/143‑1 (MQ=255)                                                                                             cGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGAGGAAGGCATTCCGGTTCGCGTGTGGGGGGGAGACGCCGGTCGCGGTCCCTCCTTCCACCGCCgcgc                                 >  1:326179/1‑143 (MQ=255)                                                                                                 aGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTg                             >  1:779560/1‑143 (MQ=255)                                                                                                    ctgttgCTGTCGGTGATAAACTGTTCGACTGGAGAGGTGCGGAAACCCTCGCTTACGCCACGCTGGTTGCTGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATc                          <  1:498274/138‑1 (MQ=255)                                                                                                             ccGGTGAGAAAGTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAg                 <  1:600943/143‑1 (MQ=255)                                                                                                                       aCTGTTCGACTGGAGCGGGGGGGAAGTGCGCGGTTACGGCTCGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGACGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGtt       <  2:147775/143‑1 (MQ=255)                                                                                                                            tCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGGGGTGCTCCACAGCCCGAATGACAGTTCCACt  >  2:731460/1‑143 (MQ=255)                                                                                                                                    |                                                                                                                                       AGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACT  >  NC_000913/760354‑760619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑