| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,440,789 | C→A | 24.9% | intergenic (‑5/‑269) | ydbK ← / → ydbJ | putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,440,789 | 0 | C | A | 24.9% | 61.6 / 17.3 | 41 | intergenic (‑5/‑269) | ydbK/ydbJ | putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
| Reads supporting (aligned to +/- strand): ref base C (14/16); new base A (6/4); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
CCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAAC > NC_000913/1440657‑1440927 | ccGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAACAGTCATCATATGACACCCTCACAt > 2:448080/1‑143 (MQ=255) gcttCCGGCCCAGGCATCAGCCTGTTCTGTCATCGTGGAACTGGGGCTCCAAGTGGAGATCGAGATCTGTCTACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTg < 2:409372/141‑1 (MQ=255) tttCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCCATAGTAATCATATGACACCCTTACATTgc > 2:568278/1‑143 (MQ=255) tCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTCCATTccgc > 1:212488/1‑143 (MQ=255) ccAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGa > 1:190748/1‑143 (MQ=255) agccATCAGCCTGTTCTGCCATTGTGGAACTGGGGGTAATCGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAgg < 1:378057/140‑1 (MQ=255) aGCCTGTTCTGCCAGTGGGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCAc < 2:320051/143‑1 (MQ=255) ccTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGa < 1:347285/143‑1 (MQ=255) gTTCTGCCATCGTGGAACTGGGGGTAAAAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAAt < 1:334204/143‑1 (MQ=255) ttCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAAtt < 2:573266/143‑1 (MQ=255) tGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATtgct > 1:575052/1‑143 (MQ=255) gtCGTGGACCGGGGGGTGTTGGGGGGGATGTGGGTACCGTCACTGGTGCGAGATGCGACCGAAGCAACCGTGCCTTTACCGTCAATAGTGATCATATGACGTCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTgcgc < 2:489534/142‑1 (MQ=255) aCTGGTGGTAATCGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCagta < 1:80720/143‑1 (MQ=255) cTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCagtag > 1:213139/1‑143 (MQ=255) gggTAAGAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAAt < 2:520858/143‑1 (MQ=255) tAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCa > 2:59110/1‑143 (MQ=255) ggTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAGAGGCGGGGCGCACGAAATTGCTGCGCGCGCAGTGGTGATCTTTCAAtt > 1:35601/1‑143 (MQ=255) aGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa < 2:198521/143‑1 (MQ=255) aGATGGCGATAACTCCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa < 2:450613/143‑1 (MQ=255) gCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCaaa < 1:59110/143‑1 (MQ=255) ttCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCCGTAGTAATCTTTCAATTTTAGCAAATGGCTttc > 2:532497/1‑143 (MQ=255) ttCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCCTTCAATTTTAGCAAATGGCTttc > 1:234652/1‑143 (MQ=255) cgggTGCGATATGCGACCGAAGCCACCGCGCTACTCCCGTCAATAGGAGTCAGATGACACCCTTACATTCCGCCAATGAGGGGCGCACCAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATCTTAGCAAATGGCTTtcttct < 2:64779/141‑1 (MQ=255) tGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTg < 1:133677/143‑1 (MQ=255) ggTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGc < 1:401469/143‑1 (MQ=255) aaaTGGCACCGCAGCAACCACGCCCTTACCGTCAATAGTAATCATATCACCCCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTc < 2:450505/143‑1 (MQ=255) aaTGTGTCCGAAGCAACCGCGCCATTACCGTCAATAGGAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTGCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCg < 2:196369/143‑1 (MQ=255) aCCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCGGCATTTTCGCTTTtg > 1:384232/1‑143 (MQ=255) ccGAAGCAACCGCGCGATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGCGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTCCAATTTTAGCAAATGGCTATCTTCTGCATTTTCGCTTTtgt > 2:486382/1‑143 (MQ=255) cAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCAAGAAATTGCTGCGCGCCCAGTAGTAATCTTTCTATTTTAGCAAATGACTTTCTTCTGCTTTTTGGCATTAGTTTcccc > 1:168899/1‑143 (MQ=255) gcgcCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTCCATTTTCGCTTTTGTGTCCCCCACAt > 2:415134/1‑143 (MQ=255) ccATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAg > 1:524495/1‑143 (MQ=255) cAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACTAAATTGCTGTGCGCTCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaatgaat < 1:393585/143‑1 (MQ=255) cAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaatgaat < 1:69514/143‑1 (MQ=255) tAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGtt > 2:434516/1‑143 (MQ=255) gTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGtttt > 2:183098/1‑143 (MQ=255) ctactCATATGATGCCCTTACACTGCGTCAATGAGGGCCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGtttt < 1:170410/139‑1 (MQ=255) tCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCACCGTAATGATTGTTTTGCTc > 2:367828/1‑143 (MQ=255) cATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCATCCAATTTTAGCAAATGGCTTTGCACTGCATGTACGCTTCTGTGTCCCCCACATCAGCGCAGTCAACGTTTTGACCa > 2:488756/1‑143 (MQ=255) tatGAAGCCCTTACATTGCGCAAATGAGGCGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTCTACCAAATGGCTTTTTTCTTCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCaaa < 2:257624/143‑1 (MQ=255) atGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGCAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATatttggtccacccgaaa > 1:219584‑M2/1‑126 (MQ=255) | CCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAAC > NC_000913/1440657‑1440927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |