| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,852,271 | C→T | 100% | R129C (CGG→TGC) | pncA → | nicotinamidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,852,271 | 0 | C | T | 91.6% | 58.4 / ‑1.2 | 24 | R129C (CGG→TGC) | pncA | nicotinamidase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (12/10); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCACTGTGTGCAGAACAGTGAAGGCGCACAATTACATCCGTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTCGGCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACCGTGCTGGACGCGTTACAGTTAGGTTATAAGGTAAACGTGAT > NC_000913/1852141‑1852407 | tCACCGTGTCAAGAACGGTGCAGGCGCACAATTACATCCTCTACTGCACCAAAAAGCGATCGCAGCGGTGCTCGATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTCGGCAGAAAACct < 1:65424/143‑1 (MQ=255) cACTGTGTGCAGAACAGTGAAGGCGCACAATTACATCCGTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACctc > 1:23580/1‑143 (MQ=255) tgtgtgCAGAACAGTGAAGGCGCACAATTACATCCGTTACTGCACCAAAAAGCGAGCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGTCCTTTGGGAGACAACctcgct > 2:263462/1‑143 (MQ=255) gtgtgCAGAACAGTGAAGGCGCACAATTACATCCGTTAGTGCACCAAAAAGCGATCGCAGCGGTGTTTCATAAAGTCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCtctctc < 2:537428/143‑1 (MQ=255) cgAAGTCGCACAATTACTTCCGTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTAc < 2:479734/142‑1 (MQ=255) gtaGGCGCACAATTACATCCGTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTAcg < 2:41805/141‑1 (MQ=255) ttACATCCGTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAAt < 1:566051/143‑1 (MQ=255) gTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCGTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGCCTGGTTACGCGATCATGAAATCGATGAAt > 1:283510/1‑143 (MQ=255) tGCCCCGAAAAGCGCTCGCAGCGGTGTGCCATAACGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGTTAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATc < 2:435263/143‑1 (MQ=255) tGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCATTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGCATTGATc > 1:171712/1‑143 (MQ=255) cAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTAt > 1:332436/1‑143 (MQ=255) aaaaGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATgg < 1:400883/143‑1 (MQ=255) tCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGTTGACTGGTTACGCGATCATGAAATCGCTGCATTGCGCGTTATGGGACTGGCt > 2:310790/1‑143 (MQ=255) gCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTCCGTGAACttt > 1:530612/1‑143 (MQ=255) caTCATTTAGTTGACAGTCACAGATCCTTCTTTGCTAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCGACTGACTATTGCGTGAAGTTTACCGt < 1:222612/142‑1 (MQ=255) agtCCTTTAGTTGACAGTGACAGTGCCTTTTTTGATACCGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACCGt < 1:182972/141‑1 (MQ=255) gTTACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACCGTGCTGGACGCGTTACAg > 1:261289/1‑143 (MQ=255) ttACAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGCTCGTTATGGGCCTGGCTACTGACTATTGCGTGACGTTTACCGTGCTGGACGCGTTACAGt > 1:90195/1‑143 (MQ=255) cAGTGCCTTTTTTGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACCGGGCTGGACGCGGTACAGTGAg > 2:318315/1‑143 (MQ=255) ttttttGATAACGGCCGTCGGCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACCGTGCTGGACGCGTTACAGTTAGGTTATaa > 1:274639/1‑143 (MQ=255) tttGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGGTGCTGACGAGACCTTGAAGTTTACCGTGCTGGACGCGTTACAGTTAGGTTATAAGGt > 1:266254/1‑143 (MQ=255) tttGATAACGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTACTGCGTGAAGTTTACCGTGCTGGACGCGTTACAGTTAGGTTATAAGGt > 1:503878/1‑143 (MQ=255) cGGCCGTTGCCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACCGTGCTGGACGCGTTACAGTTAGGTTATAAGGTAAACGTGa < 2:332436/143‑1 (MQ=255) ggCCGTTGCCAGCCAACCTCTCTCGATGACTGGGTACGCGATCACGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACCGTGCTGGACGCGTTACAGTTAGGTTATAAGGTAAACGTGAt < 1:79873/143‑1 (MQ=255) | TCACTGTGTGCAGAACAGTGAAGGCGCACAATTACATCCGTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTCGGCAGAAAACCTCTCTCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACCGTGCTGGACGCGTTACAGTTAGGTTATAAGGTAAACGTGAT > NC_000913/1852141‑1852407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |