| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,934,844 | C→A | 100% | E491* (GAG→TAG) | zwf ← | NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,934,844 | 0 | C | A | 92.0% | 55.2 / ‑4.2 | 23 | E491* (GAG→TAG) | zwf | NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (9/12); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTT > NC_000913/1934722‑1934976 | gtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGAc < 1:222885/143‑1 (MQ=255) taataaAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGAcc < 2:506029/143‑1 (MQ=255) aaaaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTCCTACAATCTGCGCCAGCCCATGTTACCGGTAAAATAACCACAAAGGGTAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCa > 2:449499/1‑143 (MQ=255) aaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACg < 1:434940/143‑1 (MQ=255) acacggcTCGTGGGTGAATCCGCACGATGCATGATAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACgg < 2:19318/137‑1 (MQ=255) aaatGCCTCGTGGGTCAACCCGCACGAGGCCTGTAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATCTTACCGGTAAAATAACCACAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACgg < 2:52562/143‑1 (MQ=255) gTGGGTGAATCCGCACGAGGCCTGAAAGTATAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATCTTACTAAAACTCATTCCAGGAACGACCATCACGGGTAaacata > 1:530583/1‑142 (MQ=255) tgcaTCCGCACGAGGCCTGAAGGTGTAAAAATTGTTCTACAATCTGCGCAAGCCCATGTTACCGGTCAAATAACCATAGAGGATAAGCCAAGATATTACTCCAACTCATTCCAGGAACGCCCACCCCGCGCAATCCACGccgc > 2:465980/4‑141 (MQ=255) gAATCCGCACGAGGCCTGAAAGTGTAAACATTGTAATACAATCTGCGCAAGATCCTGTTACCGGTAAAATAACCATAAAGGATAACCGCAGATATTACTAAAAGTCATTAACGGAACGAACATCACGGATActcatgcccaac > 2:399988/1‑136 (MQ=255) tCCGCACGAGGCCTGAAAGGGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAg < 2:325652/143‑1 (MQ=255) gCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCa > 1:382356/1‑143 (MQ=255) cgaCAATCGGCGCAAGATCATGTCACCGGTAAAAGAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATGCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACgg < 1:150433/141‑1 (MQ=255) tACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAAACATCGCCACCGAGGCAACGGGTCCCCAGGCTCCGGCCTGATACGGt > 1:513971/1‑143 (MQ=255) tCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg > 1:174148/1‑143 (MQ=255) tGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGACCGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg < 1:246907/143‑1 (MQ=255) gcgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcgc > 1:63685/1‑143 (MQ=255) aGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGcatcat < 1:23881/143‑1 (MQ=255) ttACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTACCCAGGTTACGGCCTGATACGGTTTCGGCGCATCATTGTCCATc > 1:424745/1‑143 (MQ=255) aCCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCCGCGCATCCTTGTCCATCGc > 1:41301/1‑143 (MQ=255) gtcaacaTAGCCATAAGGGACACGCCCAGATATTACTAAACCTCATTCCAGGAAGGACCATCACGGGTAATCATCGCCACCGAGGCATCGGGTCCCCAGGTCCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCa < 2:398895/137‑1 (MQ=255) cATAAAGGATAAGCGCAGATATTACTAAACCTCATTCCACGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTaa < 1:391902/143‑1 (MQ=255) aaGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTAc < 2:382356/143‑1 (MQ=255) aGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCAttt < 1:374023/143‑1 (MQ=255) | GTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTT > NC_000913/1934722‑1934976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |