| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | 100% | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 99.0% | 57.2 / ‑6.9 | 23 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (7/15); minor base G (1/0); total (8/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTA > NC_000913/124112‑124368 | gATCCGGACAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAt < 1:548577/143‑1 (MQ=255) aagaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt > 1:250496/1‑143 (MQ=255) tACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAGGCGACAGTAAGCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCTCGCACTAGGTAACGAAAAGTAACATGGACGgtgt < 2:233695/143‑1 (MQ=255) cTGCAGTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTGATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGt > 1:556465/1‑143 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg < 2:129924/143‑1 (MQ=255) aagccAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAt < 2:94059/140‑1 (MQ=255) ccAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTATGAAAAGGAACATGGACGGTGTGCGTCATATCAGCGACCGTTTCAAtgtg > 1:572880/1‑143 (MQ=255) aaaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGcc > 1:536017/1‑143 (MQ=255) aGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTACGTCATATCAGCGACCGTATCAATGTGccag > 2:152744/1‑141 (MQ=255) gCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGGTACGGCATGGGCGACGCGGCTGAAGGTATAAACATCGCGCACGAGGTTAAGAAAATGAACATGAACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGgtg > 2:99556/1‑143 (MQ=255) aaggCGACAGGAATCCTTGCTCATACCGTTAAAGGTTGCGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTc < 2:82319/140‑1 (MQ=255) aagccTTGCTCATACCATTAAAGGTTCCGGCGTGGGCGACGCGGCTGAAGGTAAAAACATCGCGGACTAGGTTAAGAAAATGAACATGGCCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGata < 1:271277/140‑1 (MQ=255) ttcTCATACCATTAAAGGTTACGGCATGTTTGACGCGGCTTAAGGTAAAAACATCGCGCACTAGTTTAAGAAAATGAACATGGAGGGTATGTGGCACAGCCGCAACTGTTTCAATGTTCCGGTTTCTGATGCAGATATCGaaa < 1:134801/141‑1 (MQ=255) tGCTGAGACCAGTAAAGGTAACGGGATGGGCGACGCGGATGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaa < 2:227839/143‑1 (MQ=255) tcccACTAAAGGTACCGGCAGGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGc < 1:165007/141‑1 (MQ=255) aCCATTGAGCGTGACGGCCTGGGCGTCGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGATGCTGCGCACGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGcc < 2:468999/143‑1 (MQ=255) tAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACa > 1:429078/1‑143 (MQ=255) ggCATGGGCGACGCGGCCGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTccc < 1:367560/143‑1 (MQ=255) aTGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGa < 1:529104/143‑1 (MQ=255) tGGGCGTCGCGGCTGAAGGTAAAAACATCGCTCACTAGGTTAAGACAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGaa < 2:97092/143‑1 (MQ=255) tGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGaa < 1:338396/143‑1 (MQ=255) gCGACGCGGCTGAAGGTGAGACCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGt < 2:287029/143‑1 (MQ=255) aaaaaCATCGCGCAGTAGGGTAAGAAGATGAACATGGACGGTGTGCGTCATATCCGCGAGCGTTTGAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCACGGCAGGTTCTGAAGAGCATACCTa > 2:170803/1‑143 (MQ=255) | GATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTA > NC_000913/124112‑124368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |