| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,368,101 | T→C | 100% | A21A (GCT→GCC) | arnA → | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,368,101 | 0 | T | C | 95.2% | 43.5 / ‑5.6 | 21 | A21A (GCT→GCC) | arnA | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (14/5); total (15/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGG > NC_000913/2367964‑2368239 | cTACACCGATGCCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGGTTTGAAGAACAGGGGATGACTCGGTATTGAAGCCCTGCTGGCCGCCgg > 1:418765/1‑143 (MQ=255) tACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACAACGATATGGGATGCGTCGGTATTGAAGCGCTGCTGGCTGCCGGt > 2:490549/1‑143 (MQ=255) cccgcccCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCGCGGTACTGAAGCCCCGCTGGCCGCCGGTTACGAAGTTAGCGCCa > 2:388242/1‑143 (MQ=255) cgcccCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGTCCGCCGGTTACGAAACTAGCACCAtt > 2:50434/1‑143 (MQ=255) tGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGCTGCCTCGGTATTGAAGCCCCGCTGTCCGCCGGTTGCGACTTTAGCGCCATTTTAAACCATACTGa > 1:79312/1‑143 (MQ=255) aGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCTGGTTTCGACATTATCGCAATTTTTACACATaataataatt > 1:239431/1‑142 (MQ=255) aGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACAACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGCCATTAGCGCCATTTTTACCCATACTTCTAATc > 2:292159/1‑143 (MQ=255) gCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGACGCCCTGCTGGCCGCCGGTCACGAAATTAGCGCCATTGTTACCCATCCTGATAATcc > 1:345812/1‑143 (MQ=255) catccaGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGGCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTCCGGATTTAGCGGCATTATTAACCAAAAAGATAATACCGGTAAAAAAgaca > 1:151977/1‑140 (MQ=255) ggCAAGGAAAATCAATAATGAAAACCGTCGTTTTTTAAAACCACTATAGGTGATGCCTCTGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATGAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTAt < 2:119760/142‑1 (MQ=255) gAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCCTTTTTACACATACTGATAATCCCGGTGAAAAAGCCTCTTATGGTTCg > 2:569690/1‑143 (MQ=255) aTGAATAATAAAAACAATCGTTTTTGTTTACCGCGAGGTGGGATGCCGCGGAATCGAAGCCCTGCTGGCCGCCGATTACGAAATTAGCGCCATTTCTACCCATACTGAAAAAAAAAAAAAAAAAGCCTTTTATGGTTCGGTgg < 2:418765/143‑1 (MQ=255) tttttGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGTAaga > 1:410717/1‑143 (MQ=255) tttttGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAagc > 1:408249/1‑142 (MQ=255) gtGCCCACCACGAGATGGGATGCCTCGGTATTGATGCCCTGCTGGCGGCCGGTTACGAGATTAGCGCCATTTTTACCCTTACTGATCATCCCGGTGAAAAAGCCTTTTATCGTTCTGTGTCTCGTCTGGCGGCGGAAAGAGGc < 2:98732/142‑1 (MQ=255) tGGGATGCCTCGGTATGTATGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATgcg < 2:79312/143‑1 (MQ=255) gggATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATAATGACAATCCCGGTGAACAAGCCTTTTATGGATCGCTGCCTCGTCTGGCGGCGGAACGAGGAAGTCCGGTTTATgcga > 2:88187/1‑142 (MQ=255) tCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCTTACTGTTAATCCCGGTGAAAAAGCCTTTTATGGTTAGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAAc < 1:209026/143‑1 (MQ=255) ttGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCTGTTAAAAAGTTTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAat < 1:190629/143‑1 (MQ=255) ttGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGCAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTaag > 1:59880/1‑142 (MQ=255) tgctgGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCtg > 2:493105/1‑143 (MQ=255) tggccgCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTTTGGTTTGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTgtgg < 1:312843/143‑1 (MQ=255) | CTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGG > NC_000913/2367964‑2368239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |