| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,525,908 | C→G | 20.1% | intergenic (+17/+22) | yfeN → / ← yfeR | conserved outer membrane protein YfeN/putative LysR‑type DNA‑binding transcriptional regulator YfeR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,525,908 | 0 | C | G | 20.1% | 41.1 / 3.5 | 23 | intergenic (+17/+22) | yfeN/yfeR | conserved outer membrane protein YfeN/putative LysR‑type DNA‑binding transcriptional regulator YfeR |
| Reads supporting (aligned to +/- strand): ref base C (9/9); new base G (3/2); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTC > NC_000913/2525768‑2526038 | tGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTc > 1:314528/1‑143 (MQ=255) gtggtgGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCa < 2:114717/143‑1 (MQ=255) gtgtggTATGCAACATGACACTTTACTCCGGGGATGCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTCCTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAg < 2:390848/141‑1 (MQ=255) tgtggAATCCATCATCACAGATTACTACGGGGATTCAGTATCGAGATGCGGATGACAAACTGGGCGAAGCTTTTTCCCATGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGt < 2:400517/142‑1 (MQ=255) gtggAATGGAACATCACACATTAGTAGGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑AGCCCCGGCAGTa < 2:489191/143‑1 (MQ=255) tggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGAGGACAAACTGGGCGAAGATTCTTACCAGGATGCGATAACCTACTACATCACGTTTAACGTCTGATTGATTACTGCCGGC‑GCTCCCGGCAGTAt > 2:336758/1‑143 (MQ=255) aaCATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCAc < 1:319180/143‑1 (MQ=255) ttACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGCATGACGTCACGCTATATCTGa > 2:561149/1‑143 (MQ=255) actacGGGGATTCAGTATCGAGATGCGGATTGCAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATa < 2:310177/143‑1 (MQ=255) tacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATCTCTGATACa > 2:402468/1‑143 (MQ=255) aTTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATc < 2:570048/143‑1 (MQ=255) tCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GGTCGCGGCAGTTTGAATTCAGTCTATATCTGTGACAACGGATCCTCTTtcc > 1:124301/1‑143 (MQ=255) aggCGGATTACAAACTGGGCGAAGATTGTTACCAGGATGCTATAATCTACTCCATCAAATTTAACTTCTTATTGATTACTGCCGGT‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa < 2:103343/141‑1 (MQ=255) ctgATGACAAACTGGGCGAAGAGTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGcc < 2:127113/141‑1 (MQ=255) gAAGATTTTTACCGGGATGGGATAATCTTCGCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTGTATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg < 2:86615/143‑1 (MQ=255) tgctgctCTACTCCATCAAATGTTACTTCTGATTGGTTACTGCCGGG‑GGTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCAt < 2:243577/137‑1 (MQ=255) tCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCCCGTCCCATACAGCTCCAgc > 2:463400/1‑143 (MQ=255) aaTTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGCCTCGTGCCACGCCCCATAACGCTTCGGCGGCGGTg > 1:15345/1‑143 (MQ=255) attcatACTGCCGGG‑AGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAATTGAACGGtt > 1:99737/6‑143 (MQ=255) ttcatACTGCCGGG‑AGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCGCGTCCCCTAAAGCTTCAGCGGCGGTGGAAAGTGGACGGgtc > 1:261220/5‑143 (MQ=255) catACTGCCGGGAGC‑CCCGGCAGTATGAATGCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACATCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttctt > 2:505795/3‑143 (MQ=255) ttACTGCCGGG‑GCTCCCGGCAGTATTAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCCCACCGCCCTGGTCTCGTACCCCCCCCCACAAAGCTTCAGCGGCGGTGGAACGTGAACGGttcttc > 2:174367/1‑143 (MQ=255) ttACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc > 1:180898/1‑143 (MQ=255) | TGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGG‑GCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTC > NC_000913/2525768‑2526038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |