| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,667,575 | G→A | 17.5% | A91V (GCG→GTG) | hcaT ← | putative 3‑phenylpropionate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,667,575 | 0 | G | A | 17.5% | 34.8 / 2.2 | 23 | A91V (GCG→GTG) | hcaT | putative 3‑phenylpropionate transporter |
| Reads supporting (aligned to +/- strand): ref base G (8/11); new base A (2/2); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
CCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACTCCCGAGGAAACGGGCAACCAGACCTGCCCCCAA > NC_000913/2667442‑2667714 | gcgcaGGCGCAGTTTGCCGTAATGAAGCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGccc < 1:382765/140‑1 (MQ=255) attGCCGTCATCAAGCGGGAACTGCAATTACCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAACGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTCCCTCCGCCCCCGCCCACACGGCGCCAg > 2:528448/2‑143 (MQ=255) gTAATCAAGCGGGAACTGATATTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCAACGCAACGTGGGCCCCCGCGCAGAGGACGACACCaccga > 2:208006/1‑139 (MQ=255) tAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCATCTGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCACCCCCGCCCAGAAGGCGACAGCAAagag > 2:463325/1‑143 (MQ=255) tAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCACCCCCGCCCAGAAGGCGACAGCAAagag > 2:172121/1‑143 (MQ=255) aTCAAGCGGGAACTGCGTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTACCGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGaa < 2:533885/143‑1 (MQ=255) tGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCCGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAAACGCCGCCGGAACAGCGAAGAGCCAGCAGTGc > 2:343140/1‑143 (MQ=255) cACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCACAGAGAAGTGTCAGCAGTGCCAGCAcgcg > 2:415710/1‑143 (MQ=255) ttCGCCAGTGCATCGGTCAACGGTACCAGTAGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGc < 1:277980/143‑1 (MQ=255) tCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCg < 2:479559/143‑1 (MQ=255) cAGTGCACCCGTCCCCAGAACCAGTGGTGAGAAACAGCAGTTGTGGCTAACCATCACCCGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGaaa < 1:48295/143‑1 (MQ=255) aTCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGAGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGGGGCAGCCGGGCCAGCGCGGGCACGGCGGAAAGCAGGc > 2:440667/1‑143 (MQ=255) tCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGaa > 1:413329/1‑143 (MQ=255) aaCGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAgg > 1:229696/1‑143 (MQ=255) agaAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGGGTCAGCAGTGCCAGCACGCGCAAGACGGAAATCAGGCGGGAAGGCACGCCGCcgcgagac > 2:135830/1‑139 (MQ=255) gagGTGCAAGCCAATCGTCAGCAGCGTCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGGCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCCGGGCGCGATGa < 2:463493/143‑1 (MQ=255) ggTTAACGCCAATCATCACCAGTATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACCCGGGGCGCGATGAGc < 2:486567/143‑1 (MQ=255) tAACGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCaaa < 1:80126/143‑1 (MQ=255) gCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACTcc < 2:393157/143‑1 (MQ=255) gcagcaGCCCCGCCACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACTCCCGAGGAAACGGGCa < 1:60076/143‑1 (MQ=255) gCTACGTGCACCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACTCCCGAGGAAACGGGCAACCAGACCTGc < 1:463325/143‑1 (MQ=255) gCTACGTGCACCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACTCCCGAGGAAACGGGCAACCAGACCTGc < 1:172121/143‑1 (MQ=255) tGCGTCCCCGCCGAGAAGGCGAGAGCAACGATAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAATGATCGCTGACGCGGGGCGCGATGAGCAAACTCCCGAGGAAACGGGCAACCAGACCTGCCCCCaa < 2:189092/143‑1 (MQ=255) | CCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACTCCCGAGGAAACGGGCAACCAGACCTGCCCCCAA > NC_000913/2667442‑2667714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |