BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,393	A→T	11.2%	intergenic (‑119/+80)	argY ← / ← argV	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,393	0	A	T	11.2%	71.3 / 3.1	34	intergenic (‑119/+80)	argY/argV	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base A (17/13); new base T (2/2); total (19/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01

CTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATC  >  NC_000913/2818252‑2818531

ctATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                                                                           <  1:164811/143‑1 (MQ=255)

           aGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGTGCCGTTGCTAAAGCAACGTTAtc                                                                                                                                >  1:368074/1‑143 (MQ=255)

             cTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggcccgaa                                                                                                                              <  1:115133/143‑8 (MQ=18)

               aCGGATGCATCGGGAAACTTACTTTACCGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAtcctcc                                                                                                                            <  1:183130/143‑1 (MQ=255)

                    tGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGt                                                                                                                       >  2:189485/1‑143 (MQ=255)

                      cgtCGGGAAACTTACTGTACTGAAGATTTTTTGATACCGCTACGAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGc                                                                                                                     <  2:501729/141‑1 (MQ=255)

                      cATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAGGGTATCGCCCCTGGTgg                                                                                                                     >  2:495650/1‑142 (MQ=255)

                            gAAACTTCCTTTATTGCAGATTTTTTGATTCCGCTCCTACAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCgg                                                                                                               <  1:555970/143‑1 (MQ=18)

                                cttacttTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCGACGTTATCCTCCCTGGTGCCTGCGGttct                                                                                                           >  1:320856/1‑141 (MQ=255)

                                 ttactttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttatt                                                                                                          >  2:403202/1‑143 (MQ=255)

                                  tactttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttattc                                                                                                         >  1:79932/1‑142 (MQ=255)

                                      gtactGCAGATTTTTTGATACCGCTACTGAAGCCGTATCAAGTAAGAGATGGTGCGTCCGGGACGATTCTTCGCCTTGCGGCTCGCCCTTCGGGCCTTTGCTAAATCAACGTTATGCTCCCTGGTGCCTGCGGTTATTACCGc                                                                                                     <  1:122482/142‑1 (MQ=17)

                                        actGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCa                                                                                                   <  1:196585/143‑1 (MQ=255)

                                            cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTCTTACCGCCACctc                                                                                               >  2:482899/1‑143 (MQ=255)

                                            cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGCTATGCTCCCTGGCGCCTGCGGTAATTACCGCcccccc                                                                                               >  2:60098/1‑141 (MQ=17)

                                              gATGTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTTCCTGGTGCCTGCGGTTATTACCGCCACCTCtt                                                                                             <  2:302162/143‑1 (MQ=255)

                                                           cGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCCCTTCCATACCTTCATc                                                                                >  1:233201/1‑143 (MQ=255)

                                                                ctaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGATAAAGCGACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGa                                                                           >  1:470318/1‑143 (MQ=255)

                                                                    aGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGt                                                                       >  1:177785/1‑143 (MQ=255)

                                                                         tATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGGACGTa                                                                  >  1:156945/1‑143 (MQ=255)

                                                                                tAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATg                                                           >  1:189127/1‑143 (MQ=255)

                                                                                 aaGAGATGGGGCATCCGGGAGGAGTACGGGCCGTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATgg                                                          <  1:133763/143‑1 (MQ=18)

                                                                                  ggcgaTGGTGCCCCGGGGAGGATTCCTCGCCGTGCGGCTCGCGCTTCGGGCCGTTGCTATAGCGACGTTATCCTCCCTTGCGCCTGCGGTTATTACCGCCACCTCTTCCATTCCTTCATCCCTGAAAGTACGTAGATTATGGt                                                         <  2:248746/140‑1 (MQ=255)

                                                                                         gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACGCCCTGGATGCTTTTCTCGATGAGTGGAGGTAGAAGATGGTGAATCCg                                                  >  1:214620/1‑143 (MQ=14)

                                                                                            cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTCCGGGCCGTTGCTAACGCAACGTTAGCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGgga                                               <  2:3436/143‑1 (MQ=255)

                                                                                                    aggaTTACTCGCCTGGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGa                                       <  2:47344/143‑1 (MQ=255)

                                                                                                         tACTCGCCTTGCGGCTCGCTCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTc                                  <  2:180544/143‑1 (MQ=255)

                                                                                                         tACGCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTc                                  <  2:477913/143‑1 (MQ=255)

                                                                                                            tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGCTATCCTCCCTGGCGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAACGTACGTAGACTATGGTGCCCCCGGGCCACTTAGAACCTCCGa                               >  2:473796/1‑143 (MQ=18)

                                                                                                             cGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAc                              <  1:189485/143‑1 (MQ=255)

                                                                                                                            ccTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTCTTACCGCCACCTCTTCCATACCTTCATCGCTGCAAGTACGTAGAATCTGGTGCATCCGGGAGGCTCCGAACCTCCGACCGCGCGGCCCGTAGc               >  2:168862/1‑143 (MQ=18)

                                                                                                                                 ataacGTTGCTTTAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGCAAGTACGTAGAATATGGTGCCTCCGGGCGGATTCGCACCTCCGACCACTCGGCTCCTAGCCGCGt          >  2:304109/5‑143 (MQ=255)

                                                                                                                                   aacGTTGCTTTAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc        >  1:178322/3‑143 (MQ=255)

                                                                                                                                      gTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCTCCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAAGATGGTGCAGCCGGGAGGATTCGACCCTCCGACCGCTCGGTTCGTAGCCGAGTActct     <  1:415323/143‑1 (MQ=255)

                                                                                                                                         gCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc  >  1:197602/1‑143 (MQ=255)

CTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATC  >  NC_000913/2818252‑2818531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`