| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,101,655 | T→A | 11.8% | intergenic (‑32/+152) | yggN ← / ← yggL | putative EcfF/protein YggL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,101,655 | 0 | T | A | 11.8% | 82.8 / 5.9 | 34 | intergenic (‑32/+152) | yggN/yggL | putative EcfF/protein YggL |
| Reads supporting (aligned to +/- strand): ref base T (17/13); new base A (2/2); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01 | |||||||||||
CAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCG > NC_000913/3101524‑3101793 | cAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAAATGCTGGcctg < 1:468778/143‑3 (MQ=255) tCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAgc > 1:230918/1‑142 (MQ=255) tCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACGGAGAGTGGCGGCGCCATCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa > 1:575775/1‑143 (MQ=255) tCACACCGTCGCGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAAATGCTGGcctgatt < 1:558776/143‑6 (MQ=255) gtCGTCACGCGGCGTGGCGCTGCACTGGGAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCAACCAACAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGa < 2:48844/142‑1 (MQ=255) tCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGAt < 1:121899/143‑1 (MQ=255) aCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATg > 2:546127/1‑143 (MQ=255) ggTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg > 1:305183/1‑143 (MQ=255) ggTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg > 1:309007/1‑143 (MQ=255) gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGAGCAGCCGGCAGCAGGATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg > 1:397113/1‑143 (MQ=255) tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGCCCAGCATTTGCTGGCCCCAGAATGATACCAAAGTACGCGTTTTCCATGCCGGGTgc > 1:479004/1‑143 (MQ=255) ggCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcgg < 1:341690/143‑1 (MQ=255) ggCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcgg > 1:555638/1‑143 (MQ=255) gCGTGAGCGGTCGTTGCCGTCACTGAAAGTGCTGCCGCTAGCAGCATTTTGCGCATCATAGTCTTCCCTCATGAAAAAATCAGGCCAGCCTTCGCGGGCCCCAGATTGATAACAAAGTACGCGTTTCCCATGCCGGATgcggc < 2:565959/143‑1 (MQ=255) cGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTCGCTGGCCCCAGATTGATATCAAAGTACGCGGTTTCCATGCCGGATgcggcg < 2:302412/143‑1 (MQ=255) ggTCATGGCCGTGACGGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGGTTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAacac < 2:393231/143‑1 (MQ=255) tGCTGTCACTGAACGTGCTGGCGCTAGCAGCATCTTGCGCTTCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACACAGTACGCGTCATCCGGGGCTGATTCGGCGTAAACACCTTATc < 2:352007/143‑1 (MQ=255) ccGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATccc > 1:548606/1‑143 (MQ=255) tCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAACTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACACAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGcc > 2:96054/1‑143 (MQ=255) tCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTTCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGGTGCGGCGTAAACAACTTACCCCGcc > 2:145301/1‑143 (MQ=255) gAAAGTGCTGCGGTCAGCAGCCTTTGGCGCATCGTAGTCTAAAAAAAAAAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTc < 2:397577/143‑1 (MQ=37) atggctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCATATTGATAACCAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGAt < 2:567933/140‑1 (MQ=255) aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGAt < 1:546127/143‑1 (MQ=255) gctgcGACCAGCAGCATTTTGCGTATCATATTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGt < 2:179200/143‑1 (MQ=255) cagcagCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCttt > 1:326943/1‑143 (MQ=255) gcaTTTTGCGTATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAgg < 2:242988/143‑1 (MQ=255) tgcATCTTAGTCTACCCTTACGCAAAAAGCGGGTCGGCATTTGCTGGCCCCAGATTGATTACAAAGTCCGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATaa < 2:284131/142‑1 (MQ=37) catcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGc > 1:548397/1‑143 (MQ=255) catcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGAGAAGc > 1:61246/1‑143 (MQ=255) tcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGt > 1:527865/1‑143 (MQ=255) tCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTACACACCTTATCCCGGCTACGCGATCGTGCATCCTGCCAGGCCTGATAAGCGTAGCGCATCAGGCa > 2:278106/1‑143 (MQ=255) atctggggCCAGCAAATGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGAGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAAGCATGATAATCGTAGCGCATCAGAAAGTTTTGCa > 2:295351/7‑143 (MQ=255) gCCAGCAAATGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCACTTGTCa > 1:75016/1‑143 (MQ=255) aGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACCCCTTATCCCGCCTACTCGATCGTGCCTCTTTCCAGGCCTGCTAAGCGTAGCGCCGCAGGCAGTTTTGCATTTGTCATCg > 1:90710/1‑143 (MQ=39) | CAATCACATCGTCACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCG > NC_000913/3101524‑3101793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |