| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,148,446 | G→A | 13.1% | E184K (GAG→AAG) | gpr → | L‑glyceraldehyde 3‑phosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,148,446 | 0 | G | A | 13.1% | 55.4 / 3.3 | 31 | E184K (GAG→AAG) | gpr | L‑glyceraldehyde 3‑phosphate reductase |
| Reads supporting (aligned to +/- strand): ref base G (13/14); new base A (2/2); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01 | |||||||||||
TCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGAC > NC_000913/3148305‑3148588 | tctcATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTCCTCGCCAGGGCGGGCGCACAACCTGGTCGGGTTGCTGCGCGa > 1:225515/1‑143 (MQ=255) cATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAACAAATGGTCGAGTTGCTCCGCGAGTg > 2:264112/1‑143 (MQ=255) aTGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGCCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGCCGAAAATACCg > 2:552849/1‑143 (MQ=255) aaaaTACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGCGCGGACGCAAACAATGGTCGAGTTGCTCCGCGAGCGGAAAATTCCGCTg > 2:318054/1‑143 (MQ=255) tGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAGAATCCGTCGTTAATtcaccca > 2:491375/1‑139 (MQ=255) gaagaaACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCAAGTGGAAAATTCCGCTGTTAATTCATCAAcc > 1:172181/1‑143 (MQ=255) gaagaaACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCAAGTGGAAAATTCCGCTGTTAATTCATCAAcc > 1:314470/1‑143 (MQ=255) gaaACCGCCTCTGCGCCGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTc < 1:286200/143‑1 (MQ=255) aTGCGGTACCAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGGCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTgg < 1:117047/143‑1 (MQ=255) aTGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGATAGAGTTGCTGCGCGAGTGGAAACTTCCGCTGTTAATTCATCAACCTTCGTACAATGTACTGAACCGCTgg > 1:253553/1‑143 (MQ=255) cGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTg < 1:320722/143‑1 (MQ=255) aaaGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGCACAATTTACTGAACCGCGGGGTGGATaaa > 2:261295/1‑143 (MQ=255) ggTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCCGTTAATTCATCAAACTTCGTCCAATTTACTTAACCGCTGGGTGGATAAAAACgg > 2:438069/1‑143 (MQ=255) tAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGcc > 2:123496/1‑143 (MQ=255) ggCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCctgc < 1:287284/143‑1 (MQ=255) cTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAGATGGTCGAGTTGCTGACCGAGTGGAAAATTCCGCTGTTAATTCATCACCCTTCGTACAAGTTACTGCACCGGTGGGTGGCTAAAAGCGGCCTGCTGGa > 2:381737/1‑143 (MQ=255) taggTCGGGATCTACTCGTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATAc < 2:225515/140‑1 (MQ=255) gTCGGGATCTCCGCTAACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCTTCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCt < 1:80985/143‑1 (MQ=255) ctctTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCAAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACg < 2:314470/143‑1 (MQ=255) ctctTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCAAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACg < 2:172181/143‑1 (MQ=255) cgcccGCCAGAGCGGACGCAAACAATGGTCGAGTTGCTGCGCGAGTGGAACATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCGGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGt < 1:138149/139‑1 (MQ=255) gccacctcGGACGCAAATAATGGCCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCt < 1:220161/136‑1 (MQ=255) ccAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACTCTGCAAAATAACGGCGTGGGCTg > 1:84449/1‑143 (MQ=255) agagCCGACGCAAAAAATCGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGCACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTa < 1:464766/143‑1 (MQ=255) agCGGACGCCAAACCGGGTCGAGTTGCTGCGCGAGTGGAAAACTCCGCGGTTAATTCATCAACCTTCGTCCAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTAtt < 1:295904/143‑1 (MQ=255) gCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTg < 1:139446/143‑1 (MQ=255) gcaacgaaTGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTa < 2:426976/137‑1 (MQ=255) cAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGAGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATCGCGGCGTGGGCGGGCTTGCCTTTAc > 1:289838/1‑143 (MQ=255) aaTGGTCGAGTGGCTGCGCGAGTGGAACATTCCGCTGTTAACTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTActcctc < 1:200771/143‑1 (MQ=255) cGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTGTACTCCTCTGGCTc > 1:469863/1‑143 (MQ=255) tgcGCGAGTGGAAAATTCCGCTGTCACTTCATCCACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTg < 1:260985/143‑1 (MQ=255) cgAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGa < 2:73317/143‑1 (MQ=255) gAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATATGTGGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTGGCCCAGGGCTTGCTgcc > 2:500806/1‑141 (MQ=255) | TCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGCCAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGAC > NC_000913/3148305‑3148588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |