| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,034,547 | A→G | 100% | E468G (GAG→GGG) | trkH → | K(+) transporter TrkH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,034,547 | 0 | A | G | 88.0% | 72.9 / ‑1.6 | 33 | E468G (GAG→GGG) | trkH | K(+) transporter TrkH |
| Reads supporting (aligned to +/- strand): ref base A (0/4); new base G (19/9); total (19/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGAGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACAAGGGACGGACAAACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAG > NC_000913/4034408‑4034686 | gACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGt > 1:219549/1‑143 (MQ=255) ttttCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCGGTTTGGCCGTCTCCGGGTcgg > 2:340594/1‑141 (MQ=255) ttttCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGGTGCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCGAACATGCTGTTTGGTCGGCTCGGGGTGtt > 2:459848/1‑143 (MQ=255) cTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGACTGCCAACATGCGCTTTGGTCGTCTCGGGGtgtgcata > 2:509706/1‑136 (MQ=255) aGGGCTTGGCGTGGTTGCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGACAACATGCGGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGa < 1:517157/143‑1 (MQ=255) gCTTGGCGTGGTTGCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGTGTGAATGATGGAGTa > 1:73667/1‑143 (MQ=255) gCGTGGTTGCTGATAACCTTACCAGTATGATCCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACg < 1:325336/143‑1 (MQ=255) tGGTTGCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGCCTTTCTGGCGTGAATGATGGACTAGTActaga > 2:371440/1‑139 (MQ=255) ggTTGCTGTTGCCTTTATCAGTGTGTGCCCGGTGGCTAGGTTGAGCCTGATTGCCATCATGCTGTGTGGTCGTTTCGGGGTCTTTACGTTGCTGGTGCTCTTTTCCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGaa < 2:181157/143‑1 (MQ=255) gCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATCGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCGTTGCCCCGACTGTCTGGCGTCAATGCTGGAGCGATACGTgggaccg > 2:426660/1‑137 (MQ=255) gCTGATAACTTTACCAGGATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACa < 1:259166/143‑1 (MQ=255) cTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGGCTGTACATTGCTGGTGCTCTTAACACCGACTCTCTGGCGTGAATGATGGAGAAGTACGCAAAAACATAAAGTca > 2:355903/1‑142 (MQ=255) tttACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACCTTGCTGTTGCTCTTTACCCCGACTTTCTGACGTGAATGATGGAGTACTACGTGAAAACATTAATTCtt > 1:33450/1‑143 (MQ=255) tttACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGCCGCGAATGATGGTTGAGTACGTGCAAACATTAATTCtt > 1:18526/1‑143 (MQ=255) tACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAATATACTATTGGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGCCTTTCTGGCGTGAATGATGGAGTAATACGTGCAAGCATTAATTCtttt > 1:548987/1‑143 (MQ=255) tGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTGGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTcaacaa < 1:428358/143‑1 (MQ=255) gAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCGCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCCACGAg > 2:312332/1‑143 (MQ=255) ccGGTTGCTAAATGCATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGAGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACAAGggac < 1:160429/143‑1 (MQ=255) cGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACAAGggacg > 1:555633/1‑143 (MQ=255) cGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGCAGTAATAGGAGACACAATGAATATTTTTCTCAAGAAGggacg > 2:142775/1‑143 (MQ=255) ttGGATCCTGGTTGCCATCATGCTGTTTGGTCGTCTCGGGGTGTTTACATTGCTGGTGCGCTGTGCCCCGACTTTCTGGCGTGACTGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACCAGGGACGGACAAAcgcg < 2:286277/142‑1 (MQ=255) aTGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACCAGGGACGGACAAAcgcc > 2:130220/1‑142 (MQ=255) tCCTGATTGCCAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACAAGGGACGGACAAACGCGCgaga > 1:557517/1‑143 (MQ=255) cAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTTGTGCTCTTTACCCCGACTTTTTCGAGTCCAATAAGCAGAAATTCGTGGTCCCATTAACTCTTTTCTCAACAAGGGACGGACAAACGCGCGAGATTGCCTCCTa < 1:576628/143‑1 (MQ=255) cAACATGCTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATCCTCACCACCACAATGGACGGACAAACGCGCGAGATTGcctcctc > 2:406760/1‑142 (MQ=255) cATGCTTTTTGGTCGTCTCGCCGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACAAGGGACGGACAAACGCGCGAGATTGCCTCCTACCt < 2:106182/143‑1 (MQ=255) attcTGTTTGGGCGTCTCGGGCTGTGTCCATTGCTGGGGCTTTTTATACTAAGTATCTGGCGTGAATGATGGAGTGGTACGTGAGAACATGAATTCTTGCCTCAACTCGGGATGGACAAACGGGCGAGATTGCCTCCTACCTg < 2:229395/140‑1 (MQ=255) cTGTTTGGTCGTCTCGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAAAACGTGAAAACATTAATTCTTTTCTCAACAAGGGACGGACAAACGCGCGAGATTGCCTCCTACCTGGCt > 1:302826/1‑143 (MQ=255) tttGGTCGGCTCGAGGTCTTTACATTGCTGGTGCGCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACAAGGGACGGACAAACGCGCGAGATTGCCTCCTACCTGGCTTCg < 2:508125/143‑1 (MQ=255) ttcggTCGTCCCCGGGTCTTTACATTGCTGGTGCTCTTCACCACGACTGTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACAAGGGACGTACAAACGCGCGAGATTGCCTCCTACCTGGCTTCg < 1:157833/140‑1 (MQ=255) tttgtcgttTGGAGGTCTTTACATTGCTGTTGCTCTTTCCCCCGATTTTCTGGCGTGAATGATGTAGTAATACGTGAAAACATTAATTCTTTCCTCAACAAGTGTCGGATAAACGCTCGAGATTGACTCCTTCCTGGCTTCgn < 2:73667/140‑2 (MQ=255) tgtcGAGGTCGTTACCTTGCTGGTGCTCTTTACCCCGACTTGCTGGCGTGAATGATGGAGTAATACGTGGAAACATTAATTCTTTTCTCAACCAGGGTCGCACGAACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGaa < 2:316704/141‑1 (MQ=255) tcGGGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACAAGGGACGGACAAACGCGCGAGATTGCCTCCTACCTGGCTTCGGCACTGaaat > 1:2857/1‑142 (MQ=255) | GACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACCAGTATGAACCCGGTGGCTAAATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGAGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGGCGTGAATGATGGAGTAATACGTGAAAACATTAATTCTTTTCTCAACAAGGGACGGACAAACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAG > NC_000913/4034408‑4034686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |