| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,362,529 | T→C | 11.5% | intergenic (‑595/+22) | cadC ← / ← pheU | DNA‑binding transcriptional activator CadC/tRNA‑Phe |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,362,529 | 0 | T | C | 11.5% | 69.0 / 3.2 | 35 | intergenic (‑595/+22) | cadC/pheU | DNA‑binding transcriptional activator CadC/tRNA‑Phe |
| Reads supporting (aligned to +/- strand): ref base T (16/15); new base C (2/2); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01 | |||||||||||
TCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACC > NC_000913/4362391‑4362667 | tCAGGAGCAAGGTGTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAg < 2:401388/143‑1 (MQ=255)tCAGGAACAAATTTTCCCGAATTGGGACATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTGCGGTAGAATTGATTTAGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCACGGAg < 1:93493/143‑1 (MQ=255) aCAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCACGGAGGTCCGt < 1:324193/143‑1 (MQ=255) aaTTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTta < 1:472143/143‑1 (MQ=255) ttttCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTtata < 2:22718/143‑1 (MQ=255) gtttCACGAATGGGGATATGCCCGCAAATTGCTGGTTATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAAAACGCTTAACTCGTGGCGTCCTAAAACGAAAAACGGACCTCCGTGGAGGTCCGTTtata < 1:349716/142‑1 (MQ=255) ggATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTATATATGTATTTGGTGcc > 1:559957/1‑143 (MQ=255) cgcGCTAATGGCTGGTGATGTGGGGAGAATGTGGTTGAGTTCTGTAGAATTGATTTGGAGGCATAACGCATCACCCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTc < 2:280740/141‑1 (MQ=255) cAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGGCCGGACTCGGaa > 1:116880/1‑143 (MQ=255) aaTTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATc < 1:406090/143‑1 (MQ=255) ttGCTGGTGATGTGGGGAGAATCTGGTAGAGGTCGGTAGAATTGATTTGGCCGGAGTGATTTGAATGCCTGGGGTTCACCTACGAAAAACGGACCGCCGTGTATGTCCGTTTGTATGAATTTGGTGCCCGGACTCGGCATCgc > 2:257145/1‑142 (MQ=255) gTGATGTGGGGGCAATCTGGCTGAGTCCGGTAGAATTGCTTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAAGTTGGTGCCCGGACTCGGAATCGAACCAAg < 1:567445/143‑1 (MQ=255) gaATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGaat > 1:477383/1‑141 (MQ=255) gaATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTCTATATGAATTTGGTGCCCGGACTCGGCATCGAACGAAGGACACGGGGAtt > 2:426824/1‑143 (MQ=255) agcTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGAtttt < 1:412675/141‑1 (MQ=255) aTCTGGTTGAGTTCGGTAGAATTGATCTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGAtttt < 1:239711/143‑1 (MQ=255) tGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGCATCGAACCAAGGACACGGGGATTTTCaa > 1:213437/1‑143 (MQ=255) tGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATccc < 1:175684/143‑1 (MQ=255) gTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGGTTTTCAATCCCCTGCTCTa > 1:196699/1‑143 (MQ=255) aTTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGGGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCggc > 1:306202/1‑141 (MQ=255) ttGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCt > 2:177100/1‑143 (MQ=255) ttGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACAGACTAAGCt > 1:569172/1‑143 (MQ=255) cTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATAGCACCAGGGACACGGGGATTTTCAATCCCCTCCTCTACCGACTGAGCTATCCGGGCAGCggg > 1:187152/1‑143 (MQ=25) gtAAATCGTGGCGTCCTGAAATGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACgggg < 1:415334/142‑1 (MQ=255) aTCCTGGCGTCCTGACACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATCAATTTTGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCa < 1:49500/143‑1 (MQ=255) ccacaaaCGAAACACGGCCCGCCGTGGAGGTCCGTTTATATGAATTGGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTa < 2:116880/139‑1 (MQ=18) cTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACAAGCGGTTGTGCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTaa > 1:214990/1‑143 (MQ=21) gAAAAACGGACCTCCGTGGAGGTGCGTTTATATGAATTTGGTGCCCGGAGTCGGAGTCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGCCTGAGCCAGCCGGGCAACGGGGCGCGTTCGACCGTAATGTGgac > 1:556632/1‑143 (MQ=18) gAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTACACCGTGATCTGcac > 1:532725/1‑143 (MQ=255) aaCGGACCTCCACGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAtct > 1:270552/1‑143 (MQ=255) cGGACCTCCACGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGAGTTTCAATCCCCTGCTATACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACAGCACTCGGCACATCTCg > 2:426823/1‑143 (MQ=17) aCCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCa < 2:346883/143‑1 (MQ=255) cTCAGTGGAGGTCCGTTCATATGAATTTGGTGCCCGGAATCGGAATCGAACAAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc < 1:399180/143‑1 (MQ=255) tCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTCCCGGCTGAGCTATCCGGGCAACGGGGCGCCTTCAACCGTAATCCGCACATCTCgcccagc > 2:160685/1‑137 (MQ=18) tCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAGACCGTAATCTGCACATCTCATCAAcc > 2:445248/1‑143 (MQ=255) | TCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACC > NC_000913/4362391‑4362667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |