Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	460,772	T→G	100%	S629A (TCG→GCG)	lon →	Lon protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	460,772	0	T	G	100.0%	45.9 / NA	16	S629A (TCG→GCG)	lon	Lon protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/10);  total (6/10)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCC  >  NC_000913/460634‑460908                                                                                                                                           |                                                                                                                                         ggTTTCGTCTATGGTCGCGCGGGGAACGGAAAGCGTTTCGGTCAGGTAGCCGGTCTGGCGTGGTCGGATGTGGGCGGTGACTTGCTGACGATTGAAACCGCATGTCTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCt                                                                                                                                      <  2:447880/142‑1 (MQ=255)            tGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACCGACCGATACCGGTGCGCTCGGCGAAGCGa                                                                                                                           >  2:114617/1‑143 (MQ=255)                cgcgcgGATAACGAAGGCCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCa                                                                                                                       <  1:251214/143‑1 (MQ=255)                                 ccGTGGCGGTCAGGGAGCCGGTCTGGCGTGGCCGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTCCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAggcgg                                                                                                      <  1:200885/143‑1 (MQ=255)                                            aGGTAACCGCTCGGGCGTGGACGGGAGTGCGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGCGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACggtg                                                                                           <  1:462397/143‑1 (MQ=255)                                              gTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCGCGGCGAAGTGACGCAGGAGTCCATTCAGGCGGCGGTAACggtggt                                                                                         >  2:491103/1‑143 (MQ=255)                                                                    aaGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTg                                                                   <  1:310249/143‑1 (MQ=255)                                                                       tGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTAGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTgggg                                                                >  2:18507/1‑143 (MQ=255)                                                                          gCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATc                                                             >  1:569432/1‑143 (MQ=255)                                                                             gTGACTTGCTGACCATTGACACCGCCGTTCTTCCCGGTAAATCCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTACATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAAc                                                          <  1:160393/143‑1 (MQ=255)                                                                                             tGACACCGCATGTGTGCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGaaa                                          <  1:230332/143‑1 (MQ=255)                                                                                                      aTGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGCGATCAACCCTGATTTTTACGAAAAACGTGACa                                 >  2:521214/1‑143 (MQ=255)                                                                                                                      ggCAAACTGACCTATACCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAcc                 >  1:575234/1‑143 (MQ=255)                                                                                                                             tGACCTATACCGGTGCGCGCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGt          <  1:69767/143‑1 (MQ=255)                                                                                                                                 ctccaCCGGGGCGCGCCGCGACGCGCTGCAGGCGCCCTTCCAGGCGGCGTGAACGGTGGTTCGTGCGCGTGCGGACAAACTGGGGATCAACCCTGATTGTTACGAAAGACGTGACATCCACGTCCACGTACCGGAAGGTGCGa      <  2:171274/139‑1 (MQ=255)                                                                                                                                     aCCGGTGCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGcc  <  2:569432/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         CGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCC  >  NC_000913/460634‑460908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑