Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	111.7 / NA	38	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/18);  total (20/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAA  >  NC_000913/754311‑754585                                                                                                                                        |                                                                                                                                             cAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTCCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTCGAGGGTGGGCTTTGc                                                                                                                                       >  1:242429/1‑143 (MQ=255)  aagctGCGGTTGAAGCGAAGTCTGGGGCACAGGCGAACACACCCCACCCACCACTATCACGACTACCAAAAGCACCCTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCt                                                                                                                                      <  2:562598/139‑1 (MQ=255)      ttcGGTTGAAGTCAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTg                                                                                                                                  <  1:537949/141‑1 (MQ=255)        cGGTTTACGTGAAGCCTGAGTCAATGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTAGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTa                                                                                                                                <  1:58498/143‑1 (MQ=255)          gTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTCACGGCGGGTTTCGCTTTTGTagc                                                                                                                              >  2:486061/1‑141 (MQ=255)           ttGATGTGAAGTCTGTGTCAAAGATGAACACACCTTTTGAAACGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                             <  1:513112/143‑1 (MQ=255)              aagtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGATGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGcc                                                                                                                          >  1:184578/1‑143 (MQ=255)                    agCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGCAAGGGTAAAAATAACAGCTTGACCAAGATTGCCTTTCAGTATATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                                    <  1:243290/143‑1 (MQ=255)                     gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                                   <  1:170842/143‑1 (MQ=255)                        tGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCATGCCTTTCAGCACATCCAGTTCAACAGCTGTATACCCGTTGAGGGCGGGTTTTGCTTTCGCATCAGCCGTTTAAGGtc                                                                                                                >  2:65326/1‑143 (MQ=255)                                    gAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCt                                                                                                    <  1:462474/143‑1 (MQ=255)                                        acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                                <  2:139333/143‑1 (MQ=255)                                          acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTCAGGTCTCCTTAGCGCCCTATTGc                                                                                              >  1:542853/1‑143 (MQ=255)                                           ccTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTGTGCTTTTGTATCAGCCATTTAAGGTCTCCCTCGCGCCTTATTgag                                                                                             >  2:18911/1‑141 (MQ=255)                                            cTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGt                                                                                            >  1:267463/1‑143 (MQ=255)                                                tGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGa                                                                                        <  2:267463/143‑1 (MQ=255)                                                    ccGAGAGTACGGATATCACTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGc                                                                                    <  1:486061/143‑1 (MQ=255)                                                     cGAGAGAACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATACCCGTTGAGGGGGGGTTTTGCTTTTGTAGCAGCCAATTCAGGTCTCGTTAGCGCCTTGTTGAGTAAGACTGc‑c                                                                                  >  2:294752/1‑143 (MQ=255)                                                               gATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGGGTAAGACTGC‑CGGAGCTTaaa                                                                        >  1:154153/1‑143 (MQ=255)                                                                       tAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCtt                                                                <  1:386154/143‑1 (MQ=255)                                                                           aTCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAGGACTGC‑CGGAACTTAACTATGCCTACgac                                                            >  2:116803/1‑141 (MQ=255)                                                                             cTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGcaca                                                          <  1:459204/143‑1 (MQ=255)                                                                               tGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATc                                                        <  1:221049/143‑1 (MQ=255)                                                                                            cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGCATGCACTGCA‑TAAAATTTGCCTTCGCACATCCACCTGGCTTCAc                                           >  2:406663/1‑143 (MQ=255)                                                                                             tttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTGAATTTGCCTTCGCACATCAACCTGGCTTTAcg                                          >  1:23700/1‑142 (MQ=255)                                                                                              ttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAccc                                         >  1:466356/1‑143 (MQ=255)                                                                                                   cacaTCCAGTTCGACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttt                                    <  2:360896/143‑1 (MQ=255)                                                                                                       tCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGCAACTTAAATTTGCCTTTGCACATCAACCTGGCCTTACCCGTTTTTTAt                                >  1:207600/1‑143 (MQ=255)                                                                                                          aGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTg                             >  2:452826/1‑143 (MQ=255)                                                                                                                   gCTGTGTCCCCCGTGAGGGTTGGTTTTGACGTTTTTTGAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CTGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccgc                    <  2:5389/143‑1 (MQ=255)                                                                                                                    cTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGct                   >  2:470681/1‑143 (MQ=255)                                                                                                                        ttCCCCGTTGAGGGTGGGTTTTGCTTGTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCGACCTGGCTTTTCCTGTGTTTTATTTGGCTCGCCGCTCtgt               <  2:206548/142‑1 (MQ=255)                                                                                                                             cGTTGAGGGTGGGTTTTGCTTTTGTATAAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑AGGAACTTAAATTTGCCTTCGCACGTCAACCTGGCTTTCCACGTTTTATATTTTGCTAGCCGCTCTGTGAAag          >  1:172468/1‑143 (MQ=255)                                                                                                                              gTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAaga         <  2:516013/143‑1 (MQ=255)                                                                                                                              gTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATGTAAGGTCTCCTTAGCGCCTTAATGAGTAAAACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAaga         <  1:214811/143‑1 (MQ=255)                                                                                                                                 gAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg      >  1:344516/1‑143 (MQ=255)                                                                                                                                 gAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg      >  1:344948/1‑143 (MQ=255)                                                                                                                                     gTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATCTTGCTCGCCGCTCTTTGCAAGAGGGGcaa  >  2:206582/1‑143 (MQ=255)                                                                                                                                        |                                                                                                                                             CAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAA  >  NC_000913/754311‑754585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑