Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,815,859	Δ82 bp		[rph]	[rph]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3815859	3815940	82	29 [0]	[0] 29	[rph]	[rph]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3815858	0 (0.000)	27 (0.680)	23/262	0.7	100%	intergenic (‑90/+5)	pyrE/rph	orotate phosphoribosyltransferase/truncated RNase PH
	?	NC_000913	3815941 =	0 (0.000)					coding (609/687 nt)	rph	truncated RNase PH

AAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCC  >  NC_000913/3815807‑3816080                                                                                                                                       |                                                                                                                                            ttgctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctccagacaaaaaaaaggcgactcagcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTg                                                                                                                                     <  1:699784‑M2/9‑1 (MQ=255)   gctacgcgcaaatccaacaaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCa                                                                                                                                   <  1:100994‑M2/11‑1 (MQ=255)             aattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAatgat                                                                                                                         <  1:288781‑M2/21‑1 (MQ=255)               ttcaataatctggcgcttatatgttttcttgcttttgctcttcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATgc                                                                                                                       <  1:670744‑M2/23‑1 (MQ=255)                              ctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaataaggcgacgcatccgtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                                                                                        >  1:151365‑M2/106‑143 (MQ=255)                                      gtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCACCACTACg                                                                                                >  2:598957‑M2/98‑143 (MQ=255)                                           atgccttcgctcgtcatcgttcttgtctacatacaaaaaaaatgcgactcatcagtcgccttaaaattcattttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCAt                                                                                           <  2:79646‑M2/51‑1 (MQ=255)                                           aggccttcgcgcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCAt                                                                                           <  2:530424‑M2/51‑1 (MQ=255)                                             gcgttcgcgcctcctcttacttttctactgtccaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAAGGATGCGCCCGTCTTCGGTCATCACTACGTTCATGt                                                                                         <  2:157832‑M2/53‑1 (MQ=255)                                               cttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatccgtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCCTCACTACGTTCATGTCg                                                                                       >  1:136684‑M2/89‑143 (MQ=255)                                               cttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTACCCTGCACTTCAATGATGCGACAGTCTTCGGTCATCACTACGTTCAtgtag                                                                                       >  1:599677‑M2/89‑141 (MQ=255)                                                 tcgctcctcatcttacttttctacagacgaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                                                                                     <  1:314016‑M2/57‑1 (MQ=255)                                                             ttccttttctacagacaaaaaaaagtcgactcatcagtcgccttaaaaatcagtttgccagcgccgccctctgcCGTCCCCTTCACTCCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga                                                                         <  2:257456‑M2/69‑1 (MQ=255)                                                                   gtctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTc                                                                   <  2:705996‑M2/75‑1 (MQ=255)                                                                       acagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGCTGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTCGGGCAGAGTCCTCCACg                                                               >  2:447309‑M2/65‑143 (MQ=255)                                                                           acaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGGCTTCAACGTAtt                                                           >  1:714577‑M2/61‑143 (MQ=255)                                                                           acaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGCTCCTGCCCGTCTCTCCGGCCCAGTCTTCAACgtact                                                           >  2:745310‑M2/61‑141 (MQ=255)                                                                            caaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTc                                                          <  1:411577‑M2/84‑1 (MQ=255)                                                                                      gcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCaa                                                <  2:786047‑M2/94‑1 (MQ=255)                                                                                             atcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCGACGTATTCCAGATCGCAAACCGCtt                                         >  1:183767‑M2/43‑143 (MQ=255)                                                                                                  tcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCg                                    >  1:568378‑M2/38‑143 (MQ=255)                                                                                                       ttaaaaatcagtttgccagcgccgccttctgcCAAAACCGGCGAAACAATGGTGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTcac                               >  1:759349‑M2/33‑143 (MQ=255)                                                                                                            aatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCCccgttcacgtctc                          >  1:660740‑M2/28‑138 (MQ=255)                                                                                                               cggtgtgccagcgccgccttctgcCGTGCCCTGCACTTCAATGAGGCGCCCGTCTTCGGTCATCACTACGTTCTTGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCTCAAACCGCTTCGCCGTTCACAATTCCGa                       <  2:322155‑M2/119‑1 (MQ=255)                                                                                                                     gccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGCGTCTTCAACGTATTCCAGATCACAAACCGCTTCGCCGTTCGCAATTCCAACagaca                 >  2:341918‑M2/19‑141 (MQ=255)                                                                                                                      ccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACCATTCCGACAGAAAc                >  1:660981‑M2/18‑143 (MQ=255)                                                                                                                      ccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAAc                >  2:169379‑M2/18‑143 (MQ=255)                                                                                                                                  tctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCAt    <  1:346867‑M2/138‑1 (MQ=255)                                                                                                                                    tgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATcc  <  2:678560‑M2/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                            AAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCC  >  NC_000913/3815807‑3816080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑