| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 96.5% | 74.7 / NA | 29 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (11/17); minor base G (0/1); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACG > NC_000913/124106‑124376 | ggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTaa < 1:31810/143‑1 (MQ=255) aCGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaa > 1:583543/1‑143 (MQ=255) tCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCTCGCACTAGGTTAAGAAAATGa > 2:125894/1‑143 (MQ=255) tCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGa < 1:636751/143‑1 (MQ=255) gaagaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACa > 1:470468/1‑143 (MQ=255) gaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCAGGGGCGACGCGGCTGAAGGGAAAAACATCGCGCACTCGGCTAAGAAAATCAAGATgg > 1:713180/1‑143 (MQ=255) aaaTCTACGCTGCACTCAAGCAAGCGCAGGCAACCAAAGGCAAAGCGACAGTACTCCTCGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGAc < 1:601356/143‑1 (MQ=255) tCTACGCTGCATGAAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgt < 2:264033/143‑1 (MQ=255) aGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAACCATCGCGCACTAGGCTAACAAAATGAACATGGACGGTCTGCGTCATATCcgc > 1:77365/1‑143 (MQ=255) gAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCGTATCcgcg > 1:530310/1‑143 (MQ=255) cAGCAAACCAAAGACAAAGCGACAGTAATCCTTGCTCATACCATAAAAGGTTACGGCATGGGCTACGCGGCTGTAGGTAAAAACATCGCGCACTAGGATAAGAAAATGAACATGGACGATTTGCGTCAGACCCGCGCCCgcgt > 2:70814/1‑140 (MQ=255) ggAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCa > 1:785934/1‑143 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATGTCCGCGACCGtctcca > 2:383589/1‑141 (MQ=255) gAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCaa < 2:689993/143‑1 (MQ=255) ccAAAGGCAAAGCGACAGTAATCCTTGCTCATAGCATTAAAGGTTACGGCATGGGCGACGGGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCGTGTCCGCGACCGTTTCAAtgtg > 1:568890/1‑143 (MQ=255) ggCAAAGCGACAGTAAGCCATGCTCATACCATTAACGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCAGgt < 1:773708/143‑1 (MQ=255) gcgcCAGTAATCCGTGCTGATACCATTGACGGTTACGGAAAGTGCGAGGCGGCGGGAGGTAAAAACATCGCGCACTAGGTCAGGAAAATGAACATGGGCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGa < 2:88734/143‑1 (MQ=255) aGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCCTATCCCTGTCCGGGTGAGTGTGCCGGTGTCTGATGCAg > 1:144728/1‑143 (MQ=255) tCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATc < 1:381523/143‑1 (MQ=255) tGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaa < 1:116875/143‑1 (MQ=255) gCTCATATCATTAAATGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaaa < 1:423807/143‑1 (MQ=255) cTCATACCACGAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaaaa < 1:150598/143‑1 (MQ=255) aTACCATTCAAGGTTACGGCACGGTCGACGCGGCTGAAGGTAAAAACCTCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTg < 1:117954/143‑1 (MQ=255) tAAAGGTTACGGCATGGGCTACTCTGCTGAAGGTAAAAACACCGCGCAGTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACa < 1:214704/143‑1 (MQ=255) tAAAGGATACGACAGGTGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGTCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACa < 2:513359/143‑1 (MQ=255) cgGCTGAGGGTAATAACATCGCGCACTAGGTTAAGAAGATGAACATTGTCGGTGTGCGTCATATCCGCTCCCGTTTCAATGTGCCGGTGTCTGACGCAGATATCGAAAAGCTGCCGTACATCACCTTCCCGGAAGGTTCTGaa < 2:326059/143‑1 (MQ=255) cagAAGGTAAAAACATCGCGGACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAagag < 1:125894/141‑1 (MQ=255) aaGTTAAACACACCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTACGTCATATCCGAGACCGTTGCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCAt < 1:755923/143‑1 (MQ=255) ggcgcACGAGGTGAAGAAAAGGAGCATGGACGGTGGGCGCCATATCCGCGACCGTTGCAATGTGCCGGTGGCGGAGGCAGAGATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACg < 2:146775/142‑1 (MQ=255) | GGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACG > NC_000913/124106‑124376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |