Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,934,844	C→A	E491* (GAG→TAG)	zwf ←	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,934,844	0	C	A	100.0%	83.1 / NA	28	E491* (GAG→TAG)	zwf	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/15);  total (13/15)

AAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAG  >  NC_000913/1934706‑1934980                                                                                                                                           |                                                                                                                                         aaaCGTTGCAGTCAGTGGAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCGACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAAc                                                                                                                                      <  1:89230/143‑1 (MQ=255)  gaCGTTGCAGTCAGTGTAAGAAAAAGAGCCTCGTGGGGGACTCCACACGAGGCATGAAAGTGTAAAAATTGTTCTACACTCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACt                                                                                                                                     <  2:315742/142‑1 (MQ=255)    cGTTTCAGTCAGGGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCa                                                                                                                                   <  1:742019/143‑1 (MQ=255)     ggttCAGGCAGGGTATTAGAAAAAGCCTCGTTGGTGAATCCGCACGATGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCAt                                                                                                                                  <  2:310199/141‑1 (MQ=255)     gTTTCAGTCAGTGTAAGAAAAAAAGCCTCGTGGGTGGAGCCGCACGAGGCCTGAAAGTGTAAAAATTGGTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACGATAAAGGATAAGCGCAGATATTACTAAAACTCAt                                                                                                                                  <  2:785803/143‑1 (MQ=255)                tgtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGa                                                                                                                       >  1:677326/1‑143 (MQ=255)                      aaaaaagGCCTCGTGGGTGAATCCGCACGGGGCCTGAAAGTGTAACAATTGTTCTACAATCTGCGCCAGCTCATGTTACCGGTAAAATAACCATAAAGGACAAGCGCAGATATTACTAAAACCCCTTCAAGGAACGCCCATCa                                                                                                                 >  2:40227/1‑143 (MQ=255)                       aaaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCAc                                                                                                                >  1:461390/1‑143 (MQ=255)                                      gtcagtCCGCACGCGGCCTGAAATTGTAAAAATTGTTCTACAATCTGCGCAAAATCTTGTAACCGGTAAAATAACCATAACTGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCa                                                                                                 <  1:349019/138‑1 (MQ=255)                                          aTCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGATCGACCATCTCAGGTTATCACCGCCACCGa                                                                                             <  2:124416/143‑1 (MQ=255)                                           tCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAg                                                                                            <  1:553323/143‑1 (MQ=255)                                               cACGAGGCCTGAACGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATACTACTAAAACTCATTCCAGGAACGACCATCACGGGTCACCACCGCCCCCGAGGCaa                                                                                        >  2:702557/1‑143 (MQ=255)                                                   aGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCGACggg                                                                                    >  1:54806/1‑143 (MQ=255)                                                                    gaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCt                                                                   <  2:622843/142‑1 (MQ=255)                                                                            tACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGCCCCCAGGTCCCGGCCTgccccagc                                                           >  2:553328/1‑136 (MQ=255)                                                                                aTCTGGGCAAGATCATGTTACCGGTAAAATAACCATAAACGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCg                                                       <  2:451587/143‑1 (MQ=255)                                                                                   tGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTGTCGgcg                                                    >  1:621963/1‑143 (MQ=255)                                                                                    gcgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcgc                                                   <  1:672981/143‑1 (MQ=255)                                                                                       cAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGcatc                                                >  1:230499/1‑143 (MQ=255)                                                                                       cAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGCGTGCTGCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGcatc                                                >  1:792573/1‑143 (MQ=255)                                                                                           aTCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGAGATGACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTg                                            >  1:589404/1‑143 (MQ=255)                                                                                               tGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCAGCGCCACCGAGGCAACGGGACCCCCGGATCCGGGCTGATACGGTTCCGACGGATCATCGTCCa                                        >  2:686217/1‑143 (MQ=255)                                                                                                 ttACCGGTAAAATAACCATAAAGGATAAGCGCAGATATCACTAACACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATc                                      <  1:69977/143‑1 (MQ=255)                                                                                                         gaaaTAACCATAAGGGACGAGCTCAGCTATGACTAAAGCTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGc                              <  2:463178/142‑1 (MQ=255)                                                                                                                  aTAAAGGGTAAGCGCAGATAGTACGAACACGCAGTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTTATACGGTTGCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAt                     <  2:242843/143‑1 (MQ=255)                                                                                                                     aaGGATAAGCGCAGATATTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGa                  >  1:106983/1‑143 (MQ=255)                                                                                                                                   atatTACTAAAACTCATTCCAGGAACGACCATCACGGGTAATCATTGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGACGCATCATTGTCCATCGCCCACGCCTCAGCAATGGAGTCTACCCCTTTcc    >  1:229903/1‑143 (MQ=255)                                                                                                                                     atTACTAAACCTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAg  <  2:306388/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         AAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAG  >  NC_000913/1934706‑1934980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑