Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	112.4 / NA	38	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/18);  total (20/18)

GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG  >  NC_000913/2132654‑2132923                                                                                                                                      |                                                                                                                                         ggagagGGTAAACCGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                 <  2:37702/141‑1 (MQ=255)                   atatTTTTTGCTGGCGCGGTCGCATTTGTCTTTGTGCTTGCGGTCAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACg                                                                                                               >  2:250597/1‑143 (MQ=255)                             cTGGCGCGGACGAATTTGTCTTTGTGCTTGCGGTAAGAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGCGATTGATATGCAGGCTCTGCGTCGACTCTTCTGCCTTCAGCCGCTCGCCGTa                                                                                                     >  2:55932/1‑143 (MQ=255)                                gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                                  <  1:503534/143‑1 (MQ=255)                                  gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca                                                                                                >  2:57572/1‑143 (MQ=255)                                          aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGACGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACCTTCCTCGGTTCGCCGTGCTCCCCCACCCTc                                                                                        >  1:731092/1‑143 (MQ=255)                                           tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATcc                                                                                       >  1:226239/1‑143 (MQ=255)                                                   tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg                                                                               >  2:502884/1‑143 (MQ=255)                                                       cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACAGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGt                                                                           >  1:121424/1‑143 (MQ=255)                                                        ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCCCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGagctc                                                                          >  2:795270/1‑139 (MQ=255)                                                         tGCGGTAAAAGTGGAAGTACCAGGAAAATTTTTTCTGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACCCCACCACCCTCCGCAGGAAGCCGTCg                                                                         >  2:98612/1‑143 (MQ=255)                                                                aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGACGATGTCGTAATCCt                                                                  >  1:671313/1‑143 (MQ=255)                                                                   gTGGACGTACGCGGGGAATTTTTTCGGTGACTAGGTGATCTGCATTTCAAAGTGATAGAGATTCATGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                               <  2:91731/143‑1 (MQ=255)                                                                      gAAGTACCCGGAGAATTTGTTCGGTGACGAGGTGATCTGCATTTCACAGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACAATCCGCAGGAAGATGGCGTAATCCGGGGCCg                                                            >  2:702551/1‑143 (MQ=255)                                                                        aGTACCCGGAGAGGCTTTTCGGTCACGGCGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCt                                                          <  1:23467/143‑1 (MQ=255)                                                                          tACCCGGAGAGTGTGTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                        <  1:290853/143‑1 (MQ=255)                                                                             cggtagaATTTTCGCCGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                     <  2:18191/139‑1 (MQ=255)                                                                              cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTGTGCGTCGCCTCTTCTACTTTCCACGGTACACCGAACTCCACCACCATGCGCAGGATGCTGGCAGAATCCTGTGCCCCGTTTAGt                                                    >  2:45068/1‑143 (MQ=255)                                                                              cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTGTGCGTCGCCGCTTCTACTTTCCACGGTTCGCCGTACTACACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt                                                    >  1:84857/1‑143 (MQ=255)                                                                                 tgttttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCGCTGCGGCGCCTCTACTACTTACCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                 <  1:251960/139‑1 (MQ=255)                                                                                 gtatgtgttttGGTGTCGTGGTGATCTGCATTTCGCCGTCATTGATATGCAGGCTCTGCGTCGCCTCGTCTACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                 <  2:449644/136‑1 (MQ=255)                                                                                 agaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                 <  1:502884/143‑1 (MQ=255)                                                                                  gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                                <  1:570274/143‑1 (MQ=255)                                                                                            ggTGACGAGGTGATCTGCATTTCACCGTAATTGATATGCAGGCTCTGCGTCGCCTCTTCTACATTCCACGCTTCGCCGGCCTCCACCACCATCCGCCGGAACATGTCCTAATACCGTCCCGCTTCCAGCTCGGCATAGaacaa                                      >  2:398757/1‑143 (MQ=255)                                                                                             gTGACGAGGTGATCTGCATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                     <  1:457981/143‑1 (MQ=255)                                                                                               taCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                   <  1:52699/142‑1 (MQ=255)                                                                                               gACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                   >  1:420316/1‑143 (MQ=255)                                                                                               gACGAGGTGATCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                   <  1:740710/143‑1 (MQ=255)                                                                                                   aGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACCctct                               <  1:654533/143‑1 (MQ=255)                                                                                                    ggTGATCGGCATTTCACCGTGATTGAGATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                              <  2:412528/143‑1 (MQ=255)                                                                                                            gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                      >  1:693531/1‑143 (MQ=255)                                                                                                              aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCAGAATGCTGTGCCGCTTTCAGTCAGGTAGGGAACAACCACACTGTTAAACGCCa                    >  2:161579/1‑143 (MQ=255)                                                                                                                  cACCGTGATTGACAGGCAGGCTCTGCGTCGCCTCCTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGCCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcc                <  1:721452/143‑1 (MQ=255)                                                                                                                       tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGt           <  1:700798/143‑1 (MQ=255)                                                                                                                        gattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTa          >  1:682864/1‑143 (MQ=255)                                                                                                                          ttgatATGCAGGCTCTGCGACGCCGCTGCAACTCCCCACGGTTCGCCGTACGCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACTCTCTTTGAAACGCCATGCCCAGGTaaa        <  1:209269/143‑1 (MQ=255)                                                                                                                             atatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc     >  1:282833/1‑143 (MQ=255)                                                                                                                                tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCAGTACTCCACAACCATCCGCAGGAAGATGTCGCAACCATATGCTGGTTTGAGTTCGGTATCGAACAAACAACCTTGGAAACGCCATGACCAAGAAAAGACCTg  >  2:278542/1‑143 (MQ=255)                                                                                                                                      |                                                                                                                                         GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG  >  NC_000913/2132654‑2132923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑