Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,368,101	T→C	A21A (GCT→GCC)	arnA →	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,368,101	0	T	C	93.8%	36.2 / NA	16	A21A (GCT→GCC)	arnA	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (10/5);  minor base G (0/1);  total (10/6)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTG  >  NC_000913/2367965‑2368238                                                                                                                                         |                                                                                                                                          tACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCACGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCACGGTATTGAAGCCCCGCTcgccgcggat                                                                                                                                     >  2:677207/1‑139 (MQ=255)                  ccgcccCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTGTTGAAGCCCTGCTGGCCGCCGGTTACGAGATTTGCGccct                                                                                                                    >  1:477998/1‑141 (MQ=255)                                        cAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCATACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATaagagc                                                                                              >  2:334266/1‑139 (MQ=255)                                          aGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAATCCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCgg                                                                                            <  1:781279/143‑1 (MQ=255)                                                 cGTCCATCCAGCAAGGAAAATGAATGATGAAACCCGTCGTTTTTGCCTGCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGaaaaa                                                                                     <  2:327493/143‑1 (MQ=255)                                                  gTCCATCCAGCAAGGAAAATGGATAATGAAAAGTGTCGGTGGTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGCCGTTATCTCCATTTTTACACATACTGAAAAAACCGACGaaaaaa                                                                                    >  1:373312/1‑142 (MQ=255)                                                      atccaGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTCCGCACTTAGCGCCCTTTTTACCCATACTCATAATCCCGGTAAAAAAACCtt                                                                                >  1:747463/1‑143 (MQ=255)                                                        cgaGCAAGGAAATTGCATAATTAATACCGTCGTTTTTGCCTACCACTATATGGGATGCCTCGGTATTGAAGCCTTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCtttt                                                                              <  2:299501/141‑1 (MQ=255)                                                                      gAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTGTGGCTCGGTGGCt                                                                >  2:87013/1‑143 (MQ=255)                                                                                 ccggtcgtTTTTGCCTCCGTCAATTTGGGATGGGTCGGTATTGAATCCGTGGTGTCCGCTGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATTCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTggcggc                                                     <  2:369893/140‑1 (MQ=255)                                                                                     tcgtTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAGACCTTTTATGGTTCGGTGGCTCGTCTGGCGGAGGaa                                                 >  1:165592/1‑143 (MQ=255)                                                                                                 aCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGTGGCGGCAACAAGCATTCCg                                     >  1:105873/1‑143 (MQ=255)                                                                                                  ccgcGAGATGGGATGCCTCGGTATTGGAGCCCTGCTGGCCGCCGGTCACGAAATTAGCGCCTTTTTTACCCATTCTGATATTCCTGGTTAAAAAGCCTTTTAAGGTGCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCgg                                    <  1:13186/140‑1 (MQ=255)                                                                                                                             aGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAatcatc         >  2:627103/1‑143 (MQ=255)                                                                                                                             aGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAatcatc         >  2:136911/1‑143 (MQ=255)                                                                                                                               cccTGCTGGCGGCCGGTGACGAAATTAGGGCCATGTGTGCCGATACTGAGAAGCGCGGTGAAAAAGCCTTTTATGGTGCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTTATCATCCg       <  2:757751/143‑1 (MQ=255)                                                                                                                                  tgctgGCCGCCGGTCACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTGCGGTGGCTCGTCTGGCGGCGGGAAGAGGCATTCCGGTTTATGCGCCGGGTAACGTGAATCATCCGTtg    >  2:157946/1‑143 (MQ=255)                                                                                                                                    ctggccgCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTATTATGGTTCGGTGTCTCGTCTGGCCGCGGCAAGCGGCAGTCCGGCCTATGCGCCGGATAACATTAATCATCCGCggtg  >  1:545528/1‑143 (MQ=255)                                                                                                                                         |                                                                                                                                          TACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTG  >  NC_000913/2367965‑2368238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑