Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,183,275	C→G	N677K (AAC→AAG)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,183,275	0	C	G	100.0%	129.6 / NA	48	N677K (AAC→AAG)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/30);  total (18/30)

GTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCG  >  NC_000913/4183144‑4183414                                                                                                                                    |                                                                                                                                            gTAACTCGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGAt                                                                                                                                  <  2:494524/143‑1 (MQ=255)   cacgtGCCGGAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACGACATGGACGTATCCACCCAGCAGGGGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATgg                                                                                                                                <  1:141688/139‑1 (MQ=255)     cTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCCAGCGTGCACTGATggct                                                                                                                              >  1:306156/1‑141 (MQ=255)       tggcGTAGCAGAGGCGAATCCAGCTGGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCGCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGc                                                                                                                            <  2:701014/140‑1 (MQ=255)         ccGTAGCAAAGGCCAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGa                                                                                                                          <  1:307058/143‑1 (MQ=255)             aGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGAGCCCGGTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACAt                                                                                                                      <  1:718550/143‑1 (MQ=255)             aGCAAAGCCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTCCATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACAt                                                                                                                      <  1:674950/143‑1 (MQ=255)                aaaGGCGCATCCAGGTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCa                                                                                                                   <  2:124288/143‑1 (MQ=255)                aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCCGGTGGTATGCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGCTGACGGCAAGCGCGCCATGCTGGGTGCGAACATGcc                                                                                                                   >  2:468001/1‑142 (MQ=255)                aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGCTGACGCCAAGCGTGCATTGATGGGTGCGAACATGCa                                                                                                                   >  1:569734/1‑143 (MQ=255)                    gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATTGACGTATCCACCCAGCGGGCGGCATCCGTCGGTGCATCCCTGATCCGGTTCCTGGGACCGGATGCCGCCAAGCGGACATTCATGGCTGCGAACATGCAACGt                                                                                                               >  2:647434/1‑143 (MQ=255)                    gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCCACGt                                                                                                               >  1:28720/1‑143 (MQ=255)                        aTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCGACGTCTgg                                                                                                           >  1:709229/1‑143 (MQ=255)                           ccgCTTGTTCCGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGCTGGTACCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCg                                                                                                        <  1:463454/141‑1 (MQ=255)                            aGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCAGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGGGCGAACATGCAACGTCAGGCCgc                                                                                                       >  1:16345/1‑142 (MQ=255)                              cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTAGCCACCCAGCAGGGGGGATCCGTCGGTGCGTCCCGGATCCCGTCCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTc                                                                                                     <  1:308168/143‑1 (MQ=255)                                 gTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                                                                                  <  2:545073/143‑1 (MQ=255)                                     aGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActct                                                                                              >  1:70058/1‑143 (MQ=255)                                             ccATGTTGACTACATGGAGGTATCCATCCAGCTGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCGGGAACACTATGACGCCAAGCGTGCATTGATGGGTGCGAACATTCAACGTCAGGTCGTTCCGACTCTGCGCGCTg                                                                                      <  2:229045/143‑1 (MQ=255)                                             ccAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTg                                                                                      <  2:533855/143‑1 (MQ=255)                                                     aCTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                                                                              >  1:88052/1‑143 (MQ=255)                                                          aTGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAGGCGTGCATGGACGGGTGCGAACATGCAACGTCAGGCCGTTCCGACGCTGCGCGCTGATAAGCCGCaggt                                                                         >  2:553196/1‑143 (MQ=255)                                                              aCGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGGTCATAGAACACGAAGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggctggt                                                                     >  1:168690/1‑143 (MQ=255)                                                                   tCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGCTCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTgg                                                                <  1:739451/143‑1 (MQ=255)                                                                   tCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGCCTCTGCGAGGTGAGCAGCCGCTGGTTGCTACtcg                                                                >  1:142556/1‑141 (MQ=255)                                                                      aCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGACAAGCGAGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGtct                                                             >  1:242195/1‑141 (MQ=255)                                                                      aCCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCGGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTAt                                                             <  1:446540/143‑1 (MQ=255)                                                                               gtggtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGc                                                    >  1:648166/1‑143 (MQ=255)                                                                               gtggtATCCGTCGGTGCGTCCCGGATCCCGCTCCTAGAACACGATGACGCCAAGCGTGCATTGTCGAGCGAACACATGTAACGGCAGGCGGTGCCTACGCCTCGCGCTGATGAGCCGCTGATGCGTGCTGGTAAGGGACGTgg                                                    >  2:239811/1‑142 (MQ=255)                                                                               gtgggATCCGTCGGGGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGc                                                    <  2:242195/143‑1 (MQ=255)                                                                                   tATCCGTTGTTTCCTCCCTTATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGTTGCGAACATGCAACGTCAGTCCGTTCCGTCTCTGCGCGCTGATAAGCCGCTGGTTTTTACTGGTATGGAACGTGCTGtt                                                <  2:261784/143‑1 (MQ=255)                                                                                         tCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTTCTGTTGCCGtt                                          >  1:422092/1‑143 (MQ=255)                                                                                              gCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTc                                     <  1:744861/143‑1 (MQ=255)                                                                                                  cccTGATCCCGTTCCTGGAACACGATGCCGCCAAGCGTGCTTTGTTGGGTGTGTACATGCATCGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGgt                                 <  1:454515/143‑1 (MQ=255)                                                                                                  cccTGATCCCGTGCCTGGAACACGAGGACGCTGAGCGTGCATTGATGGGTGCGAACATGCAACGTTAGGCCGTTCCGACTCTGCGCGCTGATGAGCCGACCATATGGGCTGGTATGGAACGTGCTGTTGCCGTTGACTCCGgt                                 <  2:356535/143‑1 (MQ=255)                                                                                                    cTGATCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGgtgt                               <  1:425622/143‑1 (MQ=255)                                                                                                        tCCCGTTCCTGGAACACGATGACGCCAAGCGTGCATTGATGGCTGCGAACATGCAACGCCAGGCCGTTCCGACTCTGCGCGCTGATCAGCGACGGGTGGGGACTGGTATGGAACGTGCTGTTGCCGTTGACCCCGGTGTAACt                           >  2:734313/1‑143 (MQ=255)                                                                                                          ccGTCCCGGGAACACGATGACGCCAAGCGTGCATTGAGGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGc                         <  1:270745/143‑1 (MQ=255)                                                                                                           cGTTCCGGGAACACGATGACGCCAAGCGTGCAGTGAGGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCg                        <  1:554122/143‑1 (MQ=255)                                                                                                             ttCCTGTAGCACGTGGGCGCCATGCGTGCATTGATGGGTGCGAACATGCAACGTCGGGCCGTTCCGACTCTGCGCGCTGATTCGCCGCTGGCTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGt                      <  2:39467/143‑1 (MQ=255)                                                                                                                cTGGAACACGATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGc                   <  1:276365/143‑1 (MQ=255)                                                                                                                     acacGACGACGCCAAGCGGGCAGTGATGGGTGCAAAGATGCAACGTCGGGCGGTGCGGACTGTGCGCGCTGGTACGCTGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAAc              <  1:126063/143‑1 (MQ=255)                                                                                                                         gATGACGCCGAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg          <  1:173077/143‑1 (MQ=255)                                                                                                                         gATGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg          <  1:322202/143‑1 (MQ=255)                                                                                                                           tgccGCCTAGCGTGCATTGATGGGTGCGAACATGCAGCGTCAGGCCGTTCCGCCTCTGCGCGCTGATACGCCGTTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtggtg        <  2:216582/140‑1 (MQ=255)                                                                                                                           tGACGCCAAGCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTGCTGGCGTGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGATAAACgtggtg        >  2:423697/1‑143 (MQ=255)                                                                                                                             gcgtcAAGCGTGCATTGATGGGTGCGGACATGCAGCGTCAGGCCGTGCCGGCTCTGCGCGGTGATAAGCCGCGGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtggtggt      <  2:494996/139‑1 (MQ=255)                                                                                                                                 cAAGCGTGTATTGCTGGGTGCGAACATGCGACGTCAGGTCGTTCCGCGTCTGCGCGCTGAGAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCg  <  2:250901/143‑1 (MQ=255)                                                                                                                                    |                                                                                                                                            GTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCG  >  NC_000913/4183144‑4183414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑