| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 97.0% | 82.9 / NA | 34 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (13/19); minor base C (1/0); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760346‑760621 | cAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAGACTGTTCGACTGGAGCg > 1:544638/1‑143 (MQ=255) tggaGCGCCGGCAGTAGCGGGCGAAACGCATCAGCACGGTGCCGGCAGCAGTTGAAAGGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAc < 2:307432/140‑1 (MQ=255) tGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAGTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTACGGAAAAc > 2:455360/1‑143 (MQ=255) aGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCGCGCGTTGCCAAGCTCTATGGACATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc < 2:387978/143‑1 (MQ=255) ggAGCTGGCGCAACGCATCAGCACGGTGGGGGAAGCAGTTGACATGCAGTCTCGCGTTGCCAAGATGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCa < 2:669241/143‑1 (MQ=255) ggAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGCACCCCTCGCTTACCCCa > 1:344292/1‑143 (MQ=255) ggAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCa > 1:631906/1‑143 (MQ=255) cTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGACAGGCGATGGCTGCCGGTGAGAAACTGGTCGACTGGAGCGGTGCGGAAAACCTCGCTTAcaccacacc > 2:94116/1‑140 (MQ=255) gCGAAACGCATCCGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑Gt < 1:75935/143‑1 (MQ=255) cGAAGCGTATCAGCACGGTGCCGGAAGCAGTTGGAATGCAGTCTCGCGTTGCCAAGATTGATGGCGATCGCCAGGCGAGGGCTGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑Gtt < 2:97257/143‑1 (MQ=255) cgggggcGGAAGCAGTTGAGATGCAGGCGCGCGTTGCCAAGATCTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGCCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCg < 1:24506/137‑1 (MQ=255) gcgggAAGCGGTTGAAATGCGGTCGAGCGTTGCTAAGGTTTATGGCGATCGCCGGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTc < 2:36345/140‑1 (MQ=255) gTTGAAATTCAGTCTCGCGTGTCCACAATTTATGGCCATCGCCAGGCGATCGCTGCGGCTGAGAAACTCTTCGACTGTAGCGTTGCTGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTTTCggg < 2:549997/143‑1 (MQ=255) gTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATCAAGGCATTCCGGTTGGCCTGTCggg > 2:783423/1‑143 (MQ=255) tGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTg < 1:616690/143‑1 (MQ=255) gcgtGCGGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGaa < 2:160738/140‑1 (MQ=255) tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGTGAAACTCTTCGACTGGAGCGGTGCGGAACACCTAGCTTGTGGTA‑GCGGCGATGATGCCGGCATTCCGCGTCGCCTGTCGGGTGGAGAc > 2:315149/1‑143 (MQ=255) gTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCg < 1:562650/143‑1 (MQ=255) gTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCg < 2:448177/143‑1 (MQ=255) tcGCGTTGCCAAGATTTATGGCGATCGCCACGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCCGAACACCTCCATTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCAGTCGGGTGAAGACTCCggac > 2:16341/1‑141 (MQ=255) cgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgcg < 1:641379/143‑1 (MQ=255) cAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGACAACCTCGCTTACGCCACGCTC‑GTTGATGAAGGCACTCCGGTTCGCCTGCCGGGTGAAGACTCAGGTCGCGGTACct > 2:604225/1‑143 (MQ=255) aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGCTACctt > 2:144110/1‑143 (MQ=255) aTGGCGATCGCCAGTCGATGGCTGCCGCTGAGTCAGTGTTCGACTGGAGCGGTGCGGAAAACCGCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 1:752824/143‑1 (MQ=255) gCGATGGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGc < 2:506968/143‑1 (MQ=255) ggCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGa < 2:227090/143‑1 (MQ=255) aTGGGTGCCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCca < 2:648030/143‑1 (MQ=255) tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGCTCGCCTGTCGGGTGAAGAGTCCGGTGGCGGTCCCTTCTTCCACCCCCACGCGGTGAGCCACAGcc > 2:180573/1‑143 (MQ=255) gCCGTTGAGGAACTGGTCGGCTGGAGCGGTGCGGAAAGCCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCa < 2:291353/143‑1 (MQ=255) ccGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGAt > 2:80931/1‑133 (MQ=255) ccGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGAt < 1:80931/133‑1 (MQ=255) aaaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GGTGATGAAGGCATTCCGGGTCGCCTGTCGGGTGAAGACTCCGGGCGCGGTACCTTGTTCCACCGCCCCGCGGCGCTCCACAAGCAGGCGAACgg > 2:60098/1‑143 (MQ=255) aCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCGACCGCCACGCCGTGATCCCCGACCAGGCTGACgggt > 2:739984/1‑141 (MQ=255) gACTGGAGCGGTGCGGAAAACCTCGCTTACGCCTCGTTG‑GTTGATGAAGTCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTa < 1:156617/143‑1 (MQ=255) | CAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760346‑760621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |