| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,440,789 | C→A | intergenic (‑5/‑269) | ydbK ← / → ydbJ | putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,440,789 | 0 | C | A | 88.9% | 77.3 / 1.1 | 37 | intergenic (‑5/‑269) | ydbK/ydbJ | putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base A (17/15); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
CCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACA > NC_000913/1440657‑1440928 | ccGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACAt > 2:670811/1‑143 (MQ=255) tttCCGGCACAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTgc < 1:466012/143‑1 (MQ=255) ttCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTgcg < 2:262684/143‑1 (MQ=255) cAGGCATCAGCCTGTTCTGCCATCGTGGAAATGGGGGTGGGATTGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTTCCGTCACTAGTAATCTTCTGAAACCCTTACATTGAGCAAATGAg > 1:152906/1‑143 (MQ=255) cAGGCAGCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATCGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAAGCATATGAAACCCTTACATTGCGCAAATGAg < 2:211349/143‑1 (MQ=255) tgtgCTGCCATCGTGGAACTGGGGGTCATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGaaa < 1:135180/143‑1 (MQ=255) attcatGCCATCGTGGAACTGGTGCGAGTACGAAAGATTTCCTTACCTTCCCTGGTGGGAAATGCGACCGAAGCAACCGCGCTATTACCGTCAATAGTAATTATATGTACTTCTTTCATTGCGCTAATGAGGGGCGCACGaaa < 2:428030/138‑1 (MQ=255) gtatagTGGAAATGGGGGTAATAGGGTAGATGGCGATAACTTCACAGGAGCGAAAAGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATtgctgc < 1:320610/138‑1 (MQ=255) cATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATAGCTgcg > 1:13853/1‑143 (MQ=255) cTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGGGCCATGCGACGGAAGCAACCGCGCCATCACCGTCAACAGTAATCATATGAAACCCTTACATTGCGCAAACGAGGGGCGCACGACATTGCTGCGCGCCcagcag > 2:651455/1‑143 (MQ=255) ggTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTCCTCGGCGCCCAGAAGTaagc > 2:764923/1‑141 (MQ=255) tAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCtt > 1:640680/1‑143 (MQ=255) aGGGTAGATGGCGCTAACGTCACTGGTGCGAAATGCGACCGAGGCAACCGCGCCATTACCGTCAATAGTCATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCaa < 2:173999/143‑1 (MQ=255) gggTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGAGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGGACGAAATTGCTGGGTGGCTTGTAGTAATCGTTCAAt > 1:292221/1‑143 (MQ=255) ggTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGCCACCCTTACATTGCGCACATGAGGGGCGCACGAAATTCCTGCGCGTCCGCTCGTAATCTTTCAAtt > 2:311393/1‑143 (MQ=255) atttGGCGATACCGTCACTAGAGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa < 1:275036/140‑1 (MQ=255) ggCGATAACTTCACTGGTGCGAAATGCGACCGAAGCGAGCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAAGGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTCGC‑aa > 1:345795/1‑143 (MQ=255) ctccACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACCTTGCTCAAATGAGGGCCGCACGAAAATCCTCCCCGCCCGCTCGTAACAGTTCAATCGTAGC‑AAATCGcgct > 2:46922/4‑140 (MQ=255) cTTCACTGGTGCGAAGTGCGGACGAAGCCACCGCGGCATTAGCGTGAATGGTAAGGCGATGAGTCCCTTGCATTGCGGAAATGAGGGGCGCACGTTATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCttt < 2:748697/143‑1 (MQ=255) ttCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTttc > 2:559574/1‑143 (MQ=255) tGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTg > 1:156300/1‑143 (MQ=255) gAAATGCGACTGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTGTGGC‑AAATGGCTTTCTTCTGCGtttt > 1:431825/1‑143 (MQ=255) gAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGCAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAACCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCAtttt < 1:515023/143‑1 (MQ=255) aaaTGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTc < 2:345190/143‑1 (MQ=255) cGACCGAAGCAGCCGCGCCATTCCCGTCAATAGTAATCATATGAATCCCTTACATTGCGCAAATGAGGGTCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCttt < 2:151676/143‑1 (MQ=255) gACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCtttt < 1:386066/143‑1 (MQ=255) gAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGGAGTAAGCTTTCAATTTTACC‑AAATGGCTTCTTTCTGTTTTTTTGCTTTtgtga > 1:292589/1‑142 (MQ=255) gAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCACAAGAAAGTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCACTTTTgtgt > 1:297763/1‑143 (MQ=255) aaGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAAATGGCTTTCTTCTGCATTTTCGCTTTTgtgt > 1:460567/1‑143 (MQ=255) aaGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTc > 1:425424/1‑143 (MQ=255) caCCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTccccc < 1:604906/142‑1 (MQ=255) aCCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTcccccc > 1:193798/1‑142 (MQ=255) ccGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCATTTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaat < 2:396698/143‑1 (MQ=255) tAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTg < 1:141305/143‑1 (MQ=255) aaTCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGa < 1:283473/143‑1 (MQ=255) aTCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTGTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGAt < 1:335200/143‑1 (MQ=255) tGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATATTTCAATATTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAGTGTTTTGATCAAACa > 1:382640/1‑143 (MQ=255) | CCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACA > NC_000913/1440657‑1440928 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |