| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,371,456 | G→C | 22.2% | S414T (AGT→ACT) | ycjM → | glucosylglycerate phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,371,456 | 0 | G | C | 22.2% | 29.6 / 3.6 | 18 | S414T (AGT→ACT) | ycjM | glucosylglycerate phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (6/8); new base C (2/2); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
TATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGA > NC_000913/1371356‑1371593 | tATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCCATATACATTCAAAGTAGTATTga > 2:263083/1‑142 (MQ=255) tGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCt > 1:301320/1‑143 (MQ=255) aaataaatGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAACTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTcg > 2:6580/1‑143 (MQ=255) aaataaatGTGACCTATATGGATGCGTTAAGCCGACGAGAGAGTAGCGATGAAGAACGTTGCGCCAGGTGGATCCTTGCCCATGGGGTTTGGGTATCGGTCCCCGGTGTGCCAGCGATATATAGCCACAGTATTCTTGGCTcg > 2:156890/1‑143 (MQ=255) cTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACg > 1:23638/1‑143 (MQ=255) tataTGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGc > 1:125829/1‑143 (MQ=255) tGGGTGCGGTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGgt < 1:157264/143‑1 (MQ=255) ttAAGCCGCCGTGAGCGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTATCTTTCCCCGGTGTTCCAGCGATATATATTCAATGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGaaaa < 1:156890/143‑1 (MQ=255) ttAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAACTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGaaaa < 1:6580/143‑1 (MQ=255) cgTGAGAGTAGCGATGAAGAACGTTGTGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGGCAGCGATATATATTCAAAGTAGTCTGGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGata < 2:52908/143‑1 (MQ=255) gTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAAATCGGatat > 2:27140/1‑143 (MQ=255) tggctaTGGAGAGCGTCGCGCCAGGTTTATCCGTGGCCGTGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGGTGTATATGCAGAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTg < 2:264240/138‑1 (MQ=255) gtcgcgcCGCGTTTATCCTTCCCCGTGCGGTTTCGTTACGTTTCCCCGGCGTGCCAGCGATATCTATTCCAAGTATTCTTCGCTCGCGTAATGATCACCCAGGTGTCGAACACCCCGGATATAACCGTGCGATTAACCGCaaa < 2:194781/140‑1 (MQ=255) cTTGCGCATGCGATTTTGTTGAGGTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAaga < 1:296031/143‑1 (MQ=255) cTTCCCCATCCGATTTTGTTAAGCTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATTATTACGCACGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAaga < 2:74130/143‑1 (MQ=255) aTGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAAcc < 2:301320/143‑1 (MQ=255) tGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTGCGCAGGCGTCGAAAAACTCGGATATAACCGTGGGAATAACCGTCAAGAAAATCACAGTCAGGTGATAGccc > 2:93116/1‑143 (MQ=255) gTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGa < 2:125829/143‑1 (MQ=255) | TATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGA > NC_000913/1371356‑1371593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |