| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,196,204 | G→A | 53.8% | intergenic (‑105/+128) | thiC ← / ← rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,196,204 | 0 | G | A | 53.8% | ‑3.4 / 12.0 | 13 | intergenic (‑105/+128) | thiC/rsd | phosphomethylpyrimidine synthase/regulator of sigma D |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (4/3); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
GGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGC > NC_000913/4196078‑4196337 | gggTCTGTTTTGTTGCAGTCATCGCTCATTCCAAAAAGCTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGCTCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGAt < 2:909/143‑1 (MQ=255) tCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGCCGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCCGGTATTAGCCTGATCAg > 2:101994/1‑143 (MQ=255) ttgCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCGGGTATTAGCCTGAGCAGGTTCCGCgg > 2:301119/1‑143 (MQ=255) aTAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGaa < 1:291330/143‑1 (MQ=255) gCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATaa < 1:96097/143‑1 (MQ=255) aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGCCTCa > 1:115835/1‑143 (MQ=255) gtgcggggCTGGTCAGATGACGGATGGAGCAGGGTGCGGCAGCCCCACGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAg < 2:140699/137‑1 (MQ=255) tGTCAGACGACGGAGGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTGTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATGAACGGTCTCAGCCAGTGAAGGCGCTCCgct > 2:151061/1‑141 (MQ=255) acgGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTa < 1:287000/143‑1 (MQ=255) gAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCg > 1:65806/1‑143 (MQ=255) ccgcAGGGGCGATGCGTTCACAAAATAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAAt < 1:301119/143‑1 (MQ=255) aGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGTTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTaa < 1:202336/143‑1 (MQ=255) gTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGACAGTTACGGCAGTCCGACAAGAAATTAGCCCCGCAAATGGGGCCTTGAATGTAAATTACGCGTTACCAGCGCAGAAATCAAGc > 1:213070/1‑143 (MQ=255) | GGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGC > NC_000913/4196078‑4196337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |