| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,202,409 | C→T | 23.7% | H162Y (CAC→TAC) | zraS → | sensory histidine kinase ZraS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,202,409 | 0 | C | T | 23.7% | 18.5 / 3.9 | 17 | H162Y (CAC→TAC) | zraS | sensory histidine kinase ZraS |
| Reads supporting (aligned to +/- strand): ref base C (4/9); new base T (2/2); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01 | |||||||||||
CGGATGAAATGCAGAAATTAAAGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCC > NC_000913/4202278‑4202546 | cgcaTGAAATGCAGGAAGTAAGGCCAGGGGAAGACTCCCGCTGGCGGCTGCCTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAAGGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCcg < 2:213690/140‑1 (MQ=255) gAAATGCAGAAATTAAAGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTg < 2:277784/143‑1 (MQ=255) tGCAGAAATTAAAGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAAc < 1:189192/143‑1 (MQ=255) aaaTTAAAGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGTACAATATGCCGCGCTGTAACGGCaa > 1:149758/1‑143 (MQ=255) aaaTTAAAGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCaa < 1:20230/143‑1 (MQ=255) aaaTTAAAGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTAACGTTAGTGGCGGCGAATGGCAGCGCCCTGGCGGCATGGAATGTACAATATGCCGCGCTGTAACGGCaa > 1:318934/1‑143 (MQ=255) gAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGACGGCATGGACTGCACAATCTGCCGAGCAACAAGGACAAAGGGGTGCAACATACCGGCGTGCAACAGGCTATTTTTagca > 1:118565/1‑140 (MQ=255) cTACGCCTGCACTTGAGGTCTATCGCTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAAGGCACAATATGCCGCGCCGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGcc < 1:96679/143‑1 (MQ=255) aCGCCTGCACTTGAGGTCTATCGTTTGTTCCAGACAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCGGTAACGGCAAAGCGGGACCACAAGGAGAGGCAAAACAGGCTAGTAGTAGCGCCGTTGAAGCCAg > 2:154221/1‑143 (MQ=255) ccTGCACTTGAGGGCTATCGTTTGTTCCAGCGAATGTCAGCGCCCCGGCAGCATGGAAGGCACAATATGCCGCGATGTAACCGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGCCAGTGa < 2:4747/143‑1 (MQ=255) ggTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGCCAGTGATCTGGTTGCaa < 1:23207/143‑1 (MQ=255) ttGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTgg < 1:320893/143‑1 (MQ=255) ttGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTgg < 1:191136/143‑1 (MQ=255) aGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATAccc > 1:7940/1‑143 (MQ=255) ggcATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACTGGCTATTTTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCctc > 1:176186/1‑143 (MQ=255) gAATGTACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGcc < 2:318934/143‑1 (MQ=255) gAATGTACAATATGCCGCGCTGTAACGGCAAAGCTGGGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGcc < 2:149758/143‑1 (MQ=255) | CGGATGAAATGCAGAAATTAAAGCCAGAGGAAAACTCCCGCTGGCGGCTGCTTGGGAAAACGGAAACTACGCCTGCACTTGAGGTCTATCGTTTGTTCCAGCCAATGTCAGCGCCCTGGCGGCATGGAATGCACAATATGCCGCGCTGTAACGGCAAAGCTGTGCCACAAGTAGATGCACAACAGGCTATTTTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCC > NC_000913/4202278‑4202546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |