Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	47.0 / NA	19	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/7);  total (12/7)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754323‑754584                                                                                                                            |                                                                                                                                           gaagtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGc                                                                                                                         >  1:152715/1‑143 (MQ=255)         gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                                 <  1:156360/143‑1 (MQ=255)          ccTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaacg                                                                                                                >  1:127493/1‑141 (MQ=255)                  aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCGATAACATCTTGACCCAGCGTGCCTTTAAGTATATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTCGCTTTTGTATCAGCCATTTAAGGTCTCCTTa                                                                                                        >  2:37955/1‑143 (MQ=255)                    aGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTCTCAGCCATTTAATGTCCCCTTAgc                                                                                                      >  1:85168/1‑143 (MQ=255)                              acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                            >  2:191742/1‑143 (MQ=255)                                  tttGAACCGAGCGTATGGCTATCACTCACATATTGACCATGCGTGAATTTCCGCACTAAACGTTAAACTGAGGTATCCCCGTTGAGGGTGGGTTTTGATTTTGCTACCGCGATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                                                        <  2:243587/143‑1 (MQ=255)                                                    atatCTATAACATCTTGGCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCGCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCGTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAt                                                                      <  2:35901/143‑1 (MQ=255)                                                         aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTGAGCGGAACGCCGTATTGCGTAAGACTGCCGGAACTTAAATTTGcc                                                                 >  1:317366/1‑143 (MQ=255)                                                           tAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAACTTTGCCtt                                                               >  2:126224/1‑143 (MQ=255)                                                              cATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGCACGGAAATTTGCCTTCGc                                                            >  2:238519/1‑143 (MQ=255)                                                               aTCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGATGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGca                                                           >  1:121532/1‑143 (MQ=255)                                                                 cTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGcaca                                                         >  1:54647/1‑143 (MQ=255)                                                                           cGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGc                                               <  2:265128/143‑1 (MQ=255)                                                                             tGCCTTGCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCtt                                             <  1:201033/143‑1 (MQ=255)                                                                               ccTTTCAGCACATCCAGTTCAACATCTGTAGCCCCGTGGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTCAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTa                                           <  2:1239/143‑1 (MQ=255)                                                                                                   aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTGGCGGGTTGTTTCGTAAGACTGCCGGAACTTACATTTGCCTTCGCACATCAACCTGGCTTTACCCGGTTTTTATTTCGCTcg                       >  2:193932/1‑143 (MQ=255)                                                                                                               cccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGACCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTGATTTGTCTGGCCGCCCtgtcct           >  2:309241/1‑140 (MQ=255)                                                                                                                        ggTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaa  <  2:102779/143‑1 (MQ=255)                                                                                                                            |                                                                                                                                           GAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754323‑754584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑