Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	65.5 / NA	28	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/12);  total (16/12)

ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG  >  NC_000913/2132649‑2132923                                                                                                                                           |                                                                                                                                         aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg                                                                                                                                      >  1:306335/1‑143 (MQ=255)     ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTACGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                  >  2:153730/1‑143 (MQ=255)        agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc                                                                                                                               >  1:14608/1‑143 (MQ=255)         gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCct                                                                                                                              >  1:235458/1‑143 (MQ=255)           gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCccct                                                                                                                            >  2:865564/1‑143 (MQ=255)            ggTAAATACTTGAGATTTTTTGCTGGCGCGGTCGAATTTGGCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGACtctt                                                                                                                           <  1:232701/143‑1 (MQ=255)                    gCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                                   <  1:811795/143‑1 (MQ=255)                      tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTTCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCa                                                                                                                 >  2:145601/1‑143 (MQ=255)                      tGATATTTTTTGCTGGCGCGAACGAATTTGTCTTTGTGCTTGCGATAAAAGTGGAAGTACCAGGAGAATTATTTCGGTGACGAGGTGAACTGCATTTCACCGTGATTGATATACAGGCTCTGAGTCGCCTCATCTaacatcca                                                                                                                 >  2:236727/1‑136 (MQ=255)                                      ggcgGTCGAAGTTGTCTCGGTGCGTGCGGTAAAGGTGGAAGTCCCAGGAGAATTTTTGCGGTGGCGAGGTGATCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTTGTACTTTCCACGGTTCGCCGTACTcc                                                                                                 <  2:148924/142‑1 (MQ=255)                                          gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGCACtgacaca                                                                                             >  2:878906/1‑137 (MQ=255)                                           tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGGAGTACCCGGAGCATTTTTTCGGTGACGAGGGGGTCTGCATGTCACCGTGATTGATATGGAGGCTCTGCGTCGCCTGTACTATTATCCACGGTCGGCCGTAGCccacccc                                                                                            >  2:640153/1‑141 (MQ=255)                                            tggctttGTCTTGTTGCTGGCGGTATAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCTCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCAGTACTccaccacc                                                                                           <  2:827504/139‑1 (MQ=255)                                              aaTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAt                                                                                         >  1:54803/1‑143 (MQ=255)                                                    tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCGCCACCATCCGcct                                                                                   >  1:550309/1‑141 (MQ=255)                                                      tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                 >  1:47080/1‑143 (MQ=255)                                                        tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg                                                                               >  1:431177/1‑143 (MQ=255)                                                                                       gtagttgttCGGGGACGTGGGGAGCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGCGATCCTGTGCCGCTTTCAGTTCgg                                                <  2:327060/136‑1 (MQ=255)                                                                                            ttgtCGGTGACGGGATGATCTGCGTGTCGCCGTGGTTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                           <  2:492932/140‑1 (MQ=255)                                                                                              ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                         <  2:47080/143‑1 (MQ=255)                                                                                               tCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac                                        <  1:41276/143‑1 (MQ=255)                                                                                                 ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGACTCTTCTACTTTCCACGGTTCGCAGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGacaga                                      >  2:335067/1‑139 (MQ=255)                                                                                                       gAGGTGGGCGGCATGTCACCGTGACTGATATGCATGCTCTGTGTCCCCTCTTCTACTTGCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActc                                <  2:589174/143‑1 (MQ=255)                                                                                                              tCTGCGTGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                         <  2:438037/143‑1 (MQ=255)                                                                                                                   aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAACCACTCTTTGAAACGCCa                    >  1:315968/1‑143 (MQ=255)                                                                                                                               ttgatACGCAGGCTCTGCGTCGCCTCTTCTACTCTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa        <  1:344619/143‑1 (MQ=255)                                                                                                                                   tatGCAGGCTCTGCGTCGCATCTCCTACTTTCCACGGTTCGCCGAACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGCCGCTTTCAGCCCGGTATCGAACAAACACTCTTTGAAACTCCATGCCGAGGAAAAGAcc    >  2:324284/1‑143 (MQ=255)                                                                                                                                     ttcAGGCTCCGCGTCCCCTCTTCTGCTTGGCACGTTTCGCCGTCCTCCACCCCCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg  <  2:489321/141‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG  >  NC_000913/2132649‑2132923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑