Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,368,101	T→C	A21A (GCT→GCC)	arnA →	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,368,101	0	T	C	100.0%	111.3 / NA	42	A21A (GCT→GCC)	arnA	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/16);  total (26/16)

CACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGG  >  NC_000913/2367967‑2368239                                                                                                                                       |                                                                                                                                           cacCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGTAAAATGAATAATGTAAATCGTCTTTTTTGCCTACTACGAGATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTa                                                                                                                                    <  1:210197/143‑1 (MQ=255)      aTGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCGACCACGATATGGGATGCCTCGGTATTGATGCCCTGCTGGCCGCCGGTTCCgccc                                                                                                                               >  1:519661/1‑140 (MQ=255)        gTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGCAAATGAATAATGAAAACCGTCGTTTTTGCCTCCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCCGGCCGCCGGTTACGTAAtt                                                                                                                             >  1:664454/1‑143 (MQ=255)           cgcgCCCGCCTCCGCTATTTTGTTCAGCAAGGTATCCGTCCATCCAGTAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAgc                                                                                                                          <  2:429743/143‑1 (MQ=255)           cgcgCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCAACCAGCAAGGCAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCATCGGTATTGAAGCCCTGCTGACCGGCGGTTACGAAACTAgc                                                                                                                          >  2:342136/1‑143 (MQ=255)                     cccGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTGCGAAATTAGCGCCActttct                                                                                                                >  1:859946/1‑141 (MQ=255)                     cccGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGACATTCGCGCCATTTTTa                                                                                                                >  1:859941/1‑143 (MQ=255)                       cGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTAcc                                                                                                              >  2:861893/1‑143 (MQ=255)                                    aGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATc                                                                                                 >  2:140832/1‑143 (MQ=255)                                    aGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATAAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCAGGCCGCCGGTTAACAAATTAGCGACACTTTTATCCATCCTGATCAtg                                                                                                 >  2:790595/1‑142 (MQ=255)                                     gCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAAGTAGCGCCATTTTTACCCAGACTGATAATcc                                                                                                >  2:249697/1‑143 (MQ=255)                                       aaGTTATCCGTACATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCATTATTTTGTATGCTTTGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCg                                                                                              <  1:830987/143‑1 (MQ=255)                                        tgTTTTCCGTGCATCCAGCAAGGAAATTGAATAATGAAAACCGTCGTTTTGGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCgg                                                                                             <  2:262528/142‑1 (MQ=255)                                                gTCCATCCAGCAAGGATAATGAATAATGAAAACCGTCATTTTTGCCTACTACGATATGGGATGCCTCGGTATTGTTGCCCTGCTGCCCGACGGTTACGAACTCACAGCCATTTTTCCCCATCCCGACAATCCCGGTGCAACAg                                                                                     >  2:296234/1‑143 (MQ=255)                                                    atccaGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCtt                                                                                 >  2:27133/1‑143 (MQ=255)                                                    atccaGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTAGGTATAGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGACCTTTTTACCCATACTGATAATCCCGGTGAAAGACCCtg                                                                                 >  1:834560/1‑142 (MQ=255)                                                                aactaaataatTAAAACCGTCGTCTTTGCCTACCTCGATATGTGATGCCTCGGTATTGATGCCTTTCTGGCCGCAGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGt                                                                     <  1:655646/138‑1 (MQ=255)                                                                aaaTGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAAACCCGGTGAAAAAGCCTTTTATGGTTCGGt                                                                     <  1:695922/143‑1 (MQ=255)                                                                           gAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTgg                                                          <  1:27133/143‑1 (MQ=255)                                                                               gccGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGATATTATCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGACTTTTATGGTTCGGTGGCTCGTCTggcggc                                                      <  1:267293/142‑1 (MQ=255)                                                                                 cgtcgtTTGTGCCTCCCACGCGATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTATCGTTATTTTTACTTATAATGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGcggcgg                                                    <  1:534347/143‑1 (MQ=255)                                                                                       ttttGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCCGTGGATCGTCTAGGGGCGGAAagag                                              >  2:814985/1‑143 (MQ=255)                                                                                        ttggCCTCCCACGATATGGGAGGCATCGGTATTGAAGCCCTGCTGGCCGCCGGTTATGATATTTTCGTCATTTTTACCCATACTGATAATCCGGGTGAAAAAGCCTTGTATGGTTCGGTGGCTCGGCTGGCGGCGGAAAGAgg                                             <  1:322436/140‑1 (MQ=255)                                                                                            ccTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCAtt                                         >  1:499888/1‑143 (MQ=255)                                                                                               aCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTGTATGGTTCGGTGGCTCGTCTGGCGGCGGAACGAGGTATTCCg                                      >  1:690199/1‑143 (MQ=255)                                                                                                ccACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGa                                                                                                      <  1:154244/78‑1 (MQ=255)                                                                                                ccACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGa                                                                                                      >  2:154244/1‑78 (MQ=255)                                                                                                 cACGATATGGGATGCCTCGGTATTAAAGCCCTGCTGGCCGCCGGTTACGAAATGAGCGCCAGTTGTACCCATACTGATAATCCAGGGGAAAAAGCCTATTCTGGTTAGGGAGCTGGGCTGGCGGCGGAAAAGGGAAGTCCGGt                                    >  2:524796/1‑143 (MQ=255)                                                                                                            gtGCCTCTGTATGGACGCCCTGCTGGCCGCCTGTTACGTATTTAGCGCCATTTTTCCCCAGCCTGAGAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCgg                         <  2:877013/142‑1 (MQ=255)                                                                                                            aTGCCTCGGTATTGAAGCCCTGCGTGCCGCCGGTTACGAACTTCGCGCCATTTTTACCCATACTGATAATCCCGGTGACAAAGCCTGTGATGGTTCGGTTGCCCGGCTGGCGCCGGGAAGCGGCCGTTCGGGTTGTGcggcgg                         >  2:456667/1‑143 (MQ=255)                                                                                                              cccTCGGTATTTAAGCCCTGCTGCCCGCCGGTTACGAAATTAGCACCTTTTTTACCCATACTGATAATCCCGGTGAAATAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGAt                       <  1:790595/142‑1 (MQ=255)                                                                                                                 tCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAAc                    >  1:638242/1‑143 (MQ=255)                                                                                                                    gTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAACGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGCAAGCGGCATTCCGGTCTGTGCGCCGGGTAACGtt                 >  1:240252/1‑143 (MQ=255)                                                                                                                    cgactcaaGCCCTGAGCGCCGCTGGTCACGAAATTGGCGCACTTTTTACGTATCCTGGTAATTCGGGTCAAAAAGGCTTTTATGGTTCGGTGGCTCGTATGGCGGCGGAAGGTGGCATTCCGGTTTATGCGCCGGATAACGtt                 <  2:495898/137‑1 (MQ=255)                                                                                                                     tATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTa                >  1:487785/1‑143 (MQ=255)                                                                                                                      aTTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTGTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTaa               <  2:898384/143‑1 (MQ=255)                                                                                                                        tGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAatc             >  1:615528/1‑143 (MQ=255)                                                                                                                          tttcccTGCTGGCCGCTGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAATAGTAGTTTATGTTTCGGTGGTTAGTCTGGCGGCGGAAAGAGGGATGCCGGTTTATGCGCCGGATAACGTTAatcat           <  1:287390/140‑1 (MQ=255)                                                                                                                          aaGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGATCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATCCCGGCTTATGCGCCGGAGAACGTTAatcac           >  2:693108/1‑142 (MQ=255)                                                                                                                             cccTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCg        >  2:558812/1‑143 (MQ=255)                                                                                                                                tgctgGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATTACGTTAATCATCCGCtg     >  1:741916/1‑143 (MQ=255)                                                                                                                                tgctgGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAAAGTTGATCATCCACtg     >  2:822293/1‑143 (MQ=255)                                                                                                                                   tggccgCCGGTTACGAAATTAGCGCCATTGTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTgtgg  <  2:664454/143‑1 (MQ=255)                                                                                                                                       |                                                                                                                                           CACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGG  >  NC_000913/2367967‑2368239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑