Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,634	A→C	T108P (ACC→CCC)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,634	0	A	C	98.0%	141.8 / NA	51	T108P (ACC→CCC)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (30/20);  minor base G (1/0);  total (31/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041502‑3041770                                                                                                                                     |                                                                                                                                         gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGCACAGATCCCACATGCGTTATTCTCTAAAGTGCCCATCGCGcc                                                                                                                                >  1:166810/1‑143 (MQ=255) gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAGCTGAAACAATATGCGGTATGTTCTGGTGTGCCTATCGCGcc                                                                                                                                >  1:483348/1‑143 (MQ=255) gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGcc                                                                                                                                >  1:725019/1‑143 (MQ=255)   cGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAg                                                                                                                              <  2:172915/143‑1 (MQ=255)       aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACGGAGACAATATGCGGTGTTCGCTAAAGTGCCCATCGCGCCAgacga                                                                                                                          >  2:342209/1‑143 (MQ=255)       aaaGGTAAAATGTGGAGCAATTTACGTCAGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCCGCTGACGGAACTGAAAAAATATGCGCTATTCACTAAAGTACCCACCGCTCCAgacga                                                                                                                          >  2:468157/1‑143 (MQ=255)           gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGGGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGGTCTGCGCGCAAGACGAagag                                                                                                                      >  2:592512/1‑143 (MQ=255)            tAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATCTGCGGTATTCTCTAGAGTGCCCATCGCGCCAGACGACGAGc                                                                                                                     >  2:875469/1‑143 (MQ=255)            tAAAATGTGCAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTCACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGc                                                                                                                     <  1:251495/143‑1 (MQ=255)                      aGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAAATGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGtgctgcc                                                                                                           >  2:620107/1‑142 (MQ=255)                      aGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGCGAACCGCAGCTGACTGAACTGAAAAAATATGCGGGATCCTCTACAGTCCCCATCGCGCCCGACGACGCGCGTgcgccact                                                                                                           >  2:911646/1‑139 (MQ=255)                       gCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGggctgcct                                                                                                          >  2:821213/1‑141 (MQ=255)                        cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGCACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGGGCGTGTGCTGCTTg                                                                                                         >  2:493064/1‑143 (MQ=255)                           tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg                                                                                                      <  1:772167/143‑1 (MQ=255)                                       ccGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                                                          <  2:91542/143‑1 (MQ=255)                                         gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAACGTGTGCTGCTTGGGGTTGCCGGGTTTCAg                                                                                        >  2:549740/1‑143 (MQ=255)                                                  gtGGCTTGGCATGGATTTAAGGGGGCAGCGTGCGTGAACCGCGGCTGACTGAACTGAAAAAAGATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGATGCTTGGTGTTGCCGGTTTTCAGGCGcgcgcc                                                                               <  2:723083/142‑1 (MQ=255)                                                       tttGTATTGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt                                                                          <  1:459998/143‑1 (MQ=255)                                                         tGCATGGATTGAACGGCGCATCGTTCGTGAACCGCAGCTGACTGAACTGGAAAAATATGCGGTAGTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                                                                        <  1:332556/143‑1 (MQ=255)                                                           cATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCa                                                                      >  1:843072/1‑143 (MQ=255)                                                            aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa                                                                     >  1:375265/1‑143 (MQ=255)                                                            aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGCTTTCAGGCGCGCGCCGCGCTGGCaa                                                                     >  2:292651/1‑143 (MQ=255)                                                             tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAACGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaca                                                                    >  2:9774/1‑141 (MQ=255)                                                               gATTGGACGGCGCAGTGTGGGTGTGCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCACGCTGGCAAAtc                                                                  <  2:144282/143‑1 (MQ=255)                                                                aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATACGCCAAAGTGCCCGTCGCGCACGACCCCGAGCTGGTGCTCACTGGCGCCGCCGGGTCTCAGGGGCGCCCCGCACTGCCacacac                                                                 >  2:691936/1‑138 (MQ=255)                                                                 ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTCCAGGCCCCCGCCGCGCTGGCACCtctc                                                                >  2:872093/1‑143 (MQ=255)                                                                 gttaaCTGCGCATCGTGCGCGACCCGCAGCTCGCTGAACTGAAAATATATGCGTTATTCTCTAAAGTGCCCATCGCGCCACACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtctc                                                                <  2:882023/140‑1 (MQ=255)                                                                    aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGTCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                             <  2:166810/143‑1 (MQ=255)                                                                          gcAGCGTGCGTGACCCGCAGCTGACTGAACTGAAAAAATATGCGGTATGCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                       <  2:423734/143‑1 (MQ=255)                                                                          gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                       <  2:907754/143‑1 (MQ=255)                                                                             gcgtgcgtGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCGCTAAAGTGCCCATCGCACCAGACGACGAGCGTGGGCTGCTTGGCGTTGCCGGTCTTCCGGCGCGCGCCGCGGTCGCAACTCTCTTTCGCGAACTg                                                    >  2:520137/1‑143 (MQ=255)                                                                                tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAGATCTCTTTAGCGGACTCCCt                                                 >  2:525481/1‑143 (MQ=255)                                                                                 gcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt                                                <  1:554744/143‑1 (MQ=255)                                                                                  cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCACATCTCTGTAGCGACCTGCCTTc                                               >  2:711229/1‑143 (MQ=255)                                                                                   gtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTCTTCGGTGGCGCGACGAGCCGGCCAAGCTCCTTAGCGAACTTCCTTCg                                              >  1:905811/1‑143 (MQ=255)                                                                                     caaCCGCAGCTGACTGCACTGAAAAAATATGCGTTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGCTGTTGCCCGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa                                            <  2:84842/142‑1 (MQ=255)                                                                                          gCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaaa                                       <  2:725019/143‑1 (MQ=255)                                                                                            aGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCcaaagcacaa                                     >  2:606334/1‑138 (MQ=255)                                                                                                  ctgaaccgaaAAAATATGCGGTATTCTCTAACGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                               <  2:541625/143‑1 (MQ=255)                                                                                                   tgaactgaaAAAATATGCGGTATTCTCTAACGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAg                              >  2:232485/1‑143 (MQ=255)                                                                                                    gaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGt                             >  2:478180/1‑143 (MQ=255)                                                                                                     aactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGACAGAAAAACAGGTAGTc                            >  2:857122/1‑143 (MQ=255)                                                                                                            aaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGACAAACAGGTAGTCAaagacg                     >  2:88664/1‑141 (MQ=255)                                                                                                                tatGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTGGAGCGCGCGTCGCGACGGCAAACGACTTTGGTTGACTGCCTTCGAAAGAAAAACAGGGAGTCATAGAAGgcgc                 >  1:170912/1‑143 (MQ=255)                                                                                                                   gCGGTATCCGCTAAAGTGCCGAGCGCGCCAGGCGACGAGCGTGTGAGGCAGGGTTTTGGCGGTTTTAAGGGCCAAGCCGCGCTGGCAAAGCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGAc              <  2:59884/143‑1 (MQ=255)                                                                                                                      gTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCAc           <  1:150889/143‑1 (MQ=255)                                                                                                                      gTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCAGGCATTCCGGCGCGCGCCGCGCTGGCAACTCTCTTTAGCGAACTCCCTGCGCACGACACACAGGCAGGCACAGCAGGCGCGACCAc           >  2:843441/1‑143 (MQ=255)                                                                                                                        aTTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACtt         <  2:302101/143‑1 (MQ=255)                                                                                                                           ctctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTCCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTCGc      >  2:640937/1‑143 (MQ=255)                                                                                                                            tctAACGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGATTTCAGGCGCGCGCCGCGGTGGCGAATCTCTTTAGCGAGCTGCCTTCGAAAGAAAAACAGGTATGCAAAGAAGGCGCGACCACATTGcc     >  2:77217/1‑142 (MQ=255)                                                                                                                               ccaGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATg  <  1:290004/141‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041502‑3041770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑