Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,543	C→A	P1100Q (CCG→CAG)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,543	0	C	A	100.0%	155.1 / NA	56	P1100Q (CCG→CAG)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/26);  total (30/26)

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184406‑4184681                                                                                                                                          |                                                                                                                                           ggCGTTTAAACGCCGTATCACGCCTTGTGACACGATGGCAGTTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGATTAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                       <  1:367849/143‑1 (MQ=255)             ccGTATGCAGCTTGGTGACAAGATGGCAGGTCGTCGGGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                           <  2:142041/143‑1 (MQ=255)              cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACACGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGCACCACAGCGTACTGAACAAGCCGAGCATACCGTCTCg                                                                                                                          >  2:641934/1‑143 (MQ=255)              cGTATCCAGCCTGGTGACAAGATGGCAGGGCGTCACGGTAAGAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGACGGAGGACACCGTACTGAACCAGCGGGGCGTAGCGTcgcg                                                                                                                          >  2:773220/1‑143 (MQ=255)                tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                        >  1:222809/1‑143 (MQ=255)                     aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACACGTGTGGAAGTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAGACGGTACGCCGGTAGACATCGGACTTGACCAGCTGGGCGTGCCGTCTCGGATTAAc                                                                                                                   >  2:368296/1‑143 (MQ=255)                     aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGTCGTACCGTCTCGTATGAAc                                                                                                                   >  1:398358/1‑143 (MQ=255)                     aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATAGAAGGTGTGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAc                                                                                                                   <  1:714325/143‑1 (MQ=255)                       ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGCTCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAt                                                                                                                 <  1:468809/143‑1 (MQ=255)                       acTGGTGACAAGGGGGCGGGGCGTCAGGGTAACAAGGGTGTAATTTCTAAGAGCGAGCCGATCGAGGGTGTGGCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAt                                                                                                                 <  2:444280/142‑1 (MQ=255)                            agACAATATGGCAGTTCGTCACTGTAACAGTGGTGTAACTTCTAAGATCACCCCGATCGTAGATATGCCTTACGATGAAAACGGTACTCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                            <  2:79558/142‑1 (MQ=255)                             gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCa                                                                                                           <  1:432260/143‑1 (MQ=255)                                aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGAt                                                                                                        >  2:268224/1‑143 (MQ=255)                                    tGGCCGGTCGTCACGGCAACAAGGGCCTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                    <  1:715875/143‑1 (MQ=255)                                    tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGGTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                    <  1:646267/143‑1 (MQ=255)                                     ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCg                                                                                                   >  2:717745/1‑143 (MQ=255)                                       cAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGGACATCGGCCAGTTGCTCgcg                                                                                                 >  1:241302/1‑141 (MQ=255)                                            cgtcACGGTAACAAGGGTGTAATTTCTAAGATCAAAAAAAATAAATTTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCa                                                                                            <  1:205186/143‑1 (MQ=255)                                              tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACGGTCTCGTATGAACATCGGTCAGATCCTCGAAAccgccc                                                                                          >  1:286454/1‑139 (MQ=255)                                              tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGATCCTCGAAACCCGcc                                                                                          >  1:53429/1‑143 (MQ=255)                                              tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAcc                                                                                          >  1:481663/1‑143 (MQ=255)                                              tcACGGTAACAAGGGTCTAATTTCTAAGCTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCCTACTGAACCAGCTTTGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAcc                                                                                          <  1:126609/143‑1 (MQ=255)                                                aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCAACCTg                                                                                        >  1:784410/1‑143 (MQ=255)                                                aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCCTACAATCGAAACGGTATGCTGGTGGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCAccgg                                                                                        >  1:171101/1‑141 (MQ=255)                                                 gggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  1:871991/142‑1 (MQ=255)                                                 cGGTAACAAGGGTGTAAGTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGACTCCTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  1:39323/143‑1 (MQ=255)                                                    tAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgggga                                                                                    >  1:891353/1‑141 (MQ=255)                                                      aCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATg                                                                                  >  1:364774/1‑143 (MQ=255)                                                           ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                             >  1:533659/1‑143 (MQ=255)                                                              gtAACTTCTAAGTTCTACTCGATCTAAGTTATGTCTTACGATGAATATGGTACGCCGGTAGACACCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                          <  1:407924/143‑1 (MQ=255)                                                               tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         >  1:406919/1‑143 (MQ=255)                                                               tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         >  2:354159/1‑143 (MQ=255)                                                               tAATGTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAGCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  2:759171/143‑1 (MQ=255)                                                               tAACTTCGACGATCAAGCCGGTCGAAGATATGCCTTACGATGAAAAGGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  2:481523/143‑1 (MQ=255)                                                                aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGCTCCTCCAACCCCCCCTGGATATGGCAGCGACAg                                                                        >  1:407525/1‑143 (MQ=255)                                                                  cttCTAACATCGACCCGATCGAAGATATGCCCTACGATGAAAACGGTACGCCGGTAGATATCGTACTGAACCAGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGt                                                                      <  2:809559/142‑1 (MQ=255)                                                                      tacgATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                  <  1:191045/140‑1 (MQ=255)                                                                          aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGACCATCGGTCAGACACCAGCAACCCATCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                              >  1:862202/1‑143 (MQ=255)                                                                               ccccATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                         <  1:236753/143‑1 (MQ=255)                                                                                ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGCACCGCCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                        <  2:742157/143‑1 (MQ=255)                                                                                              gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATAGGTCAGATCCTCGAAACCCACAAGGGCATGGCTGCGAAGGGTATCGGCGACAAGATCACCGCCATGCTGa                                          >  1:167950/1‑143 (MQ=255)                                                                                                cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                        >  2:392859/1‑143 (MQ=255)                                                                                                        gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAgcagca                                >  1:405325/1‑143 (MQ=255)                                                                                                           aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTATCGGCGACCAGCTCAACGCCATGCCGACACAGCAGCcagg                             >  1:297269/1‑142 (MQ=255)                                                                                                            aCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                            <  1:354159/143‑1 (MQ=255)                                                                                                                   gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                     <  1:717745/143‑1 (MQ=255)                                                                                                                     cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                     <  2:93569/141‑1 (MQ=255)                                                                                                                     cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                     >  1:93569/1‑141 (MQ=255)                                                                                                                           aCATCGTTCTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc             <  2:92091/143‑1 (MQ=255)                                                                                                                           aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCTATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc             >  1:825045/1‑143 (MQ=255)                                                                                                                            ctgcGTACTCAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg            <  2:796791/140‑1 (MQ=255)                                                                                                                               cGTACTGAGCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCCAGAAGTCGCGAAACTGCGCGAAt         >  2:371249/1‑143 (MQ=255)                                                                                                                               cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGTTATGGCTGCGAAAGGTATAGGCGACAAGATCAACGCCATGCCGAAACAGCAGCCAGAAGTCGCCAAACTGCGCCaaa         >  2:84387/1‑142 (MQ=255)                                                                                                                                 tACTGAACCAGCGGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTc       <  2:169887/143‑1 (MQ=255)                                                                                                                                    tGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCCTGCTGAAACAGCAGCCAGAAGTCGCGAAACTGCGCGAATTCacc    >  2:636898/1‑141 (MQ=255)                                                                                                                                      aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACGGGGGTATGGCTGCGAAAGGTATCGGCGACAAGCACAACGCCGTGCGGACACCGCCGCAAGGAGTCGCGACGCTGCGCGAATTCGTcga  >  1:764881/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                           GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184406‑4184681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑