| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 89.2% | 83.9 / NA | 38 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (16/17); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC > NC_000913/760348‑760624 | aaGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGt > 1:438952/1‑143 (MQ=255) ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGGTGGCTGCCGGTGTGAAACTGTTCGACTGGAGCGGTGcc > 1:487256/1‑142 (MQ=255) ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCg < 1:715400/143‑1 (MQ=255) tGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCGCGGGGCCGGAAGCAGTTGAAATGCAGTCTCGCGTGGCCAGGATTTATGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCgg < 2:416045/143‑1 (MQ=255) gtggcgcgcGTGCAGGAGCTGGGGAAACGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGTGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaaa < 2:805180/138‑1 (MQ=255) aTGAAGCGCCGGCAGGGGCGGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGGGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaaa < 2:376261/143‑1 (MQ=255) gcCGGCAGGAGCTGGCGAAACGCATCGGCGCGGTGCTGGGAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCt < 2:65692/143‑1 (MQ=255) cAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGCCTGGAGCGGTGCGGAAACCCTCGCTTACGc > 1:814234/1‑143 (MQ=255) ggCGGAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTgg < 2:663200/143‑1 (MQ=255) aaaCGCATCTGCACGGTCCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtga < 2:804538/143‑1 (MQ=255) cATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAg < 1:79789/143‑1 (MQ=255) cATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCCCGCTTACGCCACGCTGGTTGATGAAg > 1:88880/1‑143 (MQ=255) cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGACAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGGGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGGTGAAGGCAttg > 1:374252/1‑142 (MQ=255) cGGTGCCGGAAGCAGTCGAAATGCAGGCTCGCGTTGCCAAGATTTCTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAACCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCg < 2:796416/143‑1 (MQ=255) ggTGCCGGAAGCAGTGGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCACGCGATTGCTGCCGGGGAGAAACTGTGCGACTGGAGCGGTGCGGAACACCTCGCTTACGCCACCCTGGTTGCTGAAGGCATTCCgg > 1:408451/1‑143 (MQ=255) ggTGCCGGAAGCAGGTGAAATGCAGGCGCGCGTTGACTGTAGTGATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCgg < 2:643807/143‑1 (MQ=255) ccGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGAGTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAGACCTCGCTTACGCCACGCTGGTTGGTGAGAGGATACCAGTTCg > 2:553581/1‑143 (MQ=255) gggagtcAGTTGAAATGGCGTCTGGCGCTGCGAAGAATTACGGCGCGCGCCGGGCGATGGCTGCCGGTGAGAACCTGTTCGGCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc < 2:324496/137‑1 (MQ=255) ggAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCAAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCACGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCgac > 2:347258/1‑141 (MQ=255) gTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCTACTGGAGCGGTGCGGAAAACCTCGCTTACGCGACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCggg > 1:638188/1‑143 (MQ=255) tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGAAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGAc > 1:438576/1‑143 (MQ=255) cgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGGGGTGGGGACAACCTCGCTCAAGCCACGCTGGTTGATGAAGGCATTCTGGTTCGGCTGTAGGGTGACGACGCGGGTcgcg > 2:364466/1‑143 (MQ=255) gCCAAGATTTATGGCGATCGCCAGGCGATGGATGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGGTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAc > 1:747244/1‑143 (MQ=255) aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGCCTGGAGCGGTTCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTGCctt > 1:771220/1‑143 (MQ=255) gtATGGCGCCCGCCGGGCGATGGCTGCCAGGACGAAACGGTCTGCCGGGAGCGGTGCGGAAAACCTCCCTCCGGTTATGAACGTTCTTGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGATTCCGGTCGCGGTACCTTCTTcc < 2:649630/142‑1 (MQ=255) gATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCCACTGCCGCGGTGCGGCAACCCTCGCCTACGCCACGCTGGCTGGACAAGGCACGCGGAGCCACCCGTCGGGTGAACACCCCGGGCGCGGTCCCCCCGTCCACCGCCa > 2:173510/1‑143 (MQ=255) cgaGGCGGTGGCAGCCGGTGTGGTGCGGTGCGTCTGGAGCGGGGCGGACTACGGTGCTTGCGCGAGGCTGGTTGATTATGGCATTCCGGTTCGCCTGGCGGGTGAAGACTCCGGTCGCCGTACTTTCTTCCACCGCCACGCgg < 2:226513/141‑1 (MQ=255) ccgggCGCTGGCTGCCGGTGAGAGACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCgg < 2:286091/140‑1 (MQ=255) ccAGGCGGTGGCTGCCGGGGGGAAGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCgg < 2:685273/143‑1 (MQ=255) aTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAc < 1:388354/116‑1 (MQ=255) aTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAc > 2:388354/1‑116 (MQ=255) tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCCCCGCCACGCGCTGATCCCCCAcc > 1:748981/1‑143 (MQ=255) gCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTGGCGGTCCCTTGTTCCACCGCCACGGGGTGCTCCGCACCCa > 2:626218/1‑143 (MQ=255) agaACCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCTGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAAc < 2:420962/143‑1 (MQ=255) gaAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACCACCAGTCTCACg > 1:218092/1‑143 (MQ=255) tCGGCTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACt < 2:88880/143‑1 (MQ=255) tGGGGCGGTGCGGAAAACCTCGCTTACGCCTTCCGGGTTGATGAAGGCATTCCCGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTacac < 2:438576/143‑1 (MQ=255) tGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTacac > 1:492390/1‑143 (MQ=255) | AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC > NC_000913/760348‑760624 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |