Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,293,032	IS1 (–) +8 bp	100%	intergenic (‑110/‑488)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	46 (0.850)	37/264	NT	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 1293039	0 (0.000)					intergenic (‑117/‑488)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
*	?	NC_000913	1293032 =	0 (0.000)	54 (0.990)	49/264	NT	100%	intergenic (‑110/‑495)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

CGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/258048‑257908 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGA  <  NC_000913/1293039‑1292898                                                                                                                                                                                                                                                                                              CGGCATAACCTGAAGCTGAGGGAGCACCTGGCACGGCCGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCG                                                                                                                                               <  1:1062497/143‑1    CATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCA                                                                                                                                            <  1:672583/143‑1        ACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAA                                                                                                                                        <  1:912022/143‑1          CTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCCAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAACCACTGTCAATAGGTTGGAGTCATTACCCGGCACAAAAT                                                                                                                                      >  1:527941/1‑143           TGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATA                                                                                                                                     >  2:964266/1‑143                  GAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTAGGAGTCATTACCCGGCACAAAATAAAGAACA                                                                                                                              >  2:865452/1‑143                   AGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACGAGGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAA                                                                                                                             >  1:874889/1‑143                   AGGCAGCACCTGGCACAGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAA                                                                                                                             >  1:804391/1‑143                      tAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGATAATGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGTACAAAATAAAGAACAATTT                                                                                                                          <  2:123539/142‑1                      CAGCACCCGGCGCGGCTGGGACGGAAGTCGCTGTCGTTCTCAACATCGGTGGAGCTTCATGACAAAGTCATCGTGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTT                                                                                                                          <  2:243753/143‑1                        GCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTG                                                                                                                        <  2:720068/143‑1                          ACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCAGCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAA                                                                                                                      <  1:753316/143‑1                            CTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATT                                                                                                                    >  1:542313/1‑143                             tggtgCGGCTGCGACGGAAGTCCCTGTCGTTCTCAGACTCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAGCATAAAACACTATCAATAAGTTCGAGTCATTACCCTGCACAAAATAAAGAACAATTTTGAATTC                                                                                                                   <  2:1030059/138‑1                             TGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTC                                                                                                                   >  1:363784/1‑143                             TGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTC                                                                                                                   <  1:441774/143‑1                                CACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTT                                                                                                                >  2:674012/1‑143                                 tCGGCTTGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGCGCTGGAGGACATAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTA                                                                                                               <  2:803841/142‑1                                 ACGGCTGGGACGGAAGCCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGGCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTA                                                                                                               <  2:868113/143‑1                                               AGTCGCTGTCGTTCTAAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATT                                                                                                <  1:119737/143‑1                                                       TCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTTAACATAAAACACTATCATTATGTTGGAGTCATTACCCGGCACAAAAGAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACT                                                                                        <  1:72112/143‑1                                                       TCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACT                                                                                        <  1:179677/143‑1                                                         GTTCTCAAAAGCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACACTTTTGAATTCCTTACATTCATGGTC‑AGTGCACAACTaa                                                                                      >  1:390762/1‑141                                                          tgCTCAAAACCGGGGCAGCTGCATGACAAAGGCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAACAACAACTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAA                                                                                     <  2:585265/141‑1                                                          TTCTCAAATTCGGTGGGGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCGATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAA                                                                                     <  2:864303/143‑1                                                              CAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTA                                                                                 >  2:599362/1‑143                                                              CAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATCGCACAACTGACTTTA                                                                                 >  2:460444/1‑143                                                                               CATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCT                                                                >  1:812405/1‑143                                                                                   ACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACCTGCC‑GGCTGGAAGTACAACTGAATTTAAAGCTCTATTCTTACCTCAAC                                                            >  2:754914/1‑143                                                                                     AAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTAACTCAACAA                                                          >  1:994154/1‑143                                                                                            TCGGGCATTATCTGAACATAAAACACGAGCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCC                                                   <  2:620467/143‑1                                                                                                GCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCACCACAAACCACCACAAT                                               >  2:114871/1‑143                                                                                                   TTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAGTTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTCTCACCCCAACAAACCACCCCAAcaaa                                            >  2:176381/1‑139                                                                                                   TTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAAaaga                                            >  2:535488/1‑139                                                                                                    tagCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAA                                           <  2:962747/140‑1                                                                                                       CTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTT                                        >  1:280361/1‑143                                                                                                        TGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAGTTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGACTTTAAGGCTCCCTTATTACCTCAACAAACCACCCGCCTATAAGTTT                                       >  2:672747/1‑143                                                                                                        TGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTCTTATTACCTCAACAAACCACCCCAATATAAGTTT                                       >  1:512503/1‑143                                                                                                         GAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTG                                      <  1:929470/143‑1                                                                                                           ACATAAAACACCATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAG                                    <  2:123010/143‑1                                                                                                            CATAAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAACATAAGTTTGAGA                                   >  1:101190/1‑143                                                                                                               AAAACACTATCAATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCGACATAAGATTGAGAGTA                                <  2:713381/143‑1                                                                                                                        TCAATAAGTTGGAGTCATTACCCAGCACAAAATAAAGAACAATTTTGAATTCCTTAGATTCCCCGCG‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGA                       >  1:730303/1‑143                                                                                                                          AATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGC                     >  2:961798/1‑143                                                                                                                           ATAAGTTGGAGTCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCG                    >  1:714102/1‑143                                                                                                                             gaggTGGAGTCATTACCCGGGGCAAAATAAAGAACAATTTGGAATTCGTGACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATAGAAGTTTTAGATTACTACAATGAGCGAA                  <  2:1008282/139‑1                                                                                                                                TTGGAGTCATTACCAGGCACAAAATAAAGAATAATTTTGAATTCCTTACATCCCTAGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCA               <  2:522613/143‑1                                                                                                                                      TCATTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACAGATTGTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCAATTAAA         >  2:426829/1‑143                                                                                                                                         TTACCCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTAAACAAATCACCACAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATT      >  1:184003/1‑143                                                                                                                                             CCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAGCAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGA  <  1:674012/143‑1                                                                                                                                             CCGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGA  >  1:254372/1‑143                                                                                                                                                                                                                                                                                              CGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/258048‑257908 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCACAAAATAAAGAACAATTTTGAATTCCTTACATTCCTGGCT‑ATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGA  <  NC_000913/1293039‑1292898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.